Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity
Our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. The objectives were to: 1) estimate acute toxicity of cryoprotectants over a range of concentrations; 2) evaluate the properties of vitrification solutions (VS);...
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ftpubmed:oai:pubmedcentral.nih.gov:4462170 2023-05-15T18:06:04+02:00 Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity Cuevas-Uribe, Rafael Chesney, Edward J. Daly, Jonathan Tiersch, Terrence R. 2013-12-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462170/ http://www.ncbi.nlm.nih.gov/pubmed/26074721 https://doi.org/10.1111/are.12337 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462170/ http://www.ncbi.nlm.nih.gov/pubmed/26074721 http://dx.doi.org/10.1111/are.12337 Article Text 2013 ftpubmed https://doi.org/10.1111/are.12337 2016-06-05T00:12:07Z Our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. The objectives were to: 1) estimate acute toxicity of cryoprotectants over a range of concentrations; 2) evaluate the properties of vitrification solutions (VS); 3) evaluate different thawing solutions, and 4) evaluate sperm quality after thawing by examination of motility and membrane integrity. Sperm were collected from red snapper (Lutjanus campechanus), spotted seatrout (Cynoscion nebulosus), and red drum (Sciaenops ocellatus). A total of 29 combinations of cryoprotectants were evaluated for toxicity and glass formation. Samples were loaded onto 10-µL polystyrene loops and plunged into liquid nitrogen. There was a significant difference (P < 0.05) in post-thaw motility among VS and among species when using the same VS. The sperm in VS of 15% DMSO + 15% ethylene glycol + 10% glycerol + 1% X-1000™ + 1% Z-1000™ had an average post-thaw motility of 58% and membrane integrity of 19% for spotted seatrout, 38% and 9% for red snapper, and 30% and 19% for red drum. Adaptations by marine fish to high osmotic pressures could explain the survival in the high cryoprotectant concentrations. Vitrification offers an alternative to conventional cryopreservation. Text Red drum Sciaenops ocellatus PubMed Central (PMC) Aquaculture Research 46 7 1770 1784 |
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Article Cuevas-Uribe, Rafael Chesney, Edward J. Daly, Jonathan Tiersch, Terrence R. Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity |
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description |
Our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. The objectives were to: 1) estimate acute toxicity of cryoprotectants over a range of concentrations; 2) evaluate the properties of vitrification solutions (VS); 3) evaluate different thawing solutions, and 4) evaluate sperm quality after thawing by examination of motility and membrane integrity. Sperm were collected from red snapper (Lutjanus campechanus), spotted seatrout (Cynoscion nebulosus), and red drum (Sciaenops ocellatus). A total of 29 combinations of cryoprotectants were evaluated for toxicity and glass formation. Samples were loaded onto 10-µL polystyrene loops and plunged into liquid nitrogen. There was a significant difference (P < 0.05) in post-thaw motility among VS and among species when using the same VS. The sperm in VS of 15% DMSO + 15% ethylene glycol + 10% glycerol + 1% X-1000™ + 1% Z-1000™ had an average post-thaw motility of 58% and membrane integrity of 19% for spotted seatrout, 38% and 9% for red snapper, and 30% and 19% for red drum. Adaptations by marine fish to high osmotic pressures could explain the survival in the high cryoprotectant concentrations. Vitrification offers an alternative to conventional cryopreservation. |
format |
Text |
author |
Cuevas-Uribe, Rafael Chesney, Edward J. Daly, Jonathan Tiersch, Terrence R. |
author_facet |
Cuevas-Uribe, Rafael Chesney, Edward J. Daly, Jonathan Tiersch, Terrence R. |
author_sort |
Cuevas-Uribe, Rafael |
title |
Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity |
title_short |
Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity |
title_full |
Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity |
title_fullStr |
Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity |
title_full_unstemmed |
Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity |
title_sort |
vitrification of sperm from marine fishes: effect on motility and membrane integrity |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462170/ http://www.ncbi.nlm.nih.gov/pubmed/26074721 https://doi.org/10.1111/are.12337 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462170/ http://www.ncbi.nlm.nih.gov/pubmed/26074721 http://dx.doi.org/10.1111/are.12337 |
op_doi |
https://doi.org/10.1111/are.12337 |
container_title |
Aquaculture Research |
container_volume |
46 |
container_issue |
7 |
container_start_page |
1770 |
op_container_end_page |
1784 |
_version_ |
1766177637256921088 |