Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast
Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induce...
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ftpubmed:oai:pubmedcentral.nih.gov:4449880 2023-05-15T16:33:26+02:00 Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast Imazaki, Pedro Henrique Brose, François Jauniaux, Thierry Das, Krishna Muller, Marc Scippo, Marie-Louise 2015 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449880/ http://www.ncbi.nlm.nih.gov/pubmed/26075240 https://doi.org/10.1155/2015/438295 en eng Hindawi Publishing Corporation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449880/ http://www.ncbi.nlm.nih.gov/pubmed/26075240 http://dx.doi.org/10.1155/2015/438295 Copyright © 2015 Pedro Henrique Imazaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1155/2015/438295 2015-07-05T00:03:58Z Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p′-DDT was the most potent xenoestrogen from the DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only β-HCH (at 400 and 2000 ng/mL) and δ-HCH (at 2000 ng/mL) displayed estrogenic activities. These 13 organochlorines were determined by GC-MS in 12 samples of North Sea harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 × 102~4.2 × 104 μg/kg), p,p′-DDE (5.1 × 102~8.6 × 103 μg/kg), and HCB (7.6 × 101~1.5 × 103 μg/kg) were the compounds found in highest concentrations. The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts. Text Harbour porpoise Phocoena phocoena PubMed Central (PMC) BioMed Research International 2015 1 13 |
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Research Article Imazaki, Pedro Henrique Brose, François Jauniaux, Thierry Das, Krishna Muller, Marc Scippo, Marie-Louise Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
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Research Article |
description |
Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p′-DDT was the most potent xenoestrogen from the DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only β-HCH (at 400 and 2000 ng/mL) and δ-HCH (at 2000 ng/mL) displayed estrogenic activities. These 13 organochlorines were determined by GC-MS in 12 samples of North Sea harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 × 102~4.2 × 104 μg/kg), p,p′-DDE (5.1 × 102~8.6 × 103 μg/kg), and HCB (7.6 × 101~1.5 × 103 μg/kg) were the compounds found in highest concentrations. The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts. |
format |
Text |
author |
Imazaki, Pedro Henrique Brose, François Jauniaux, Thierry Das, Krishna Muller, Marc Scippo, Marie-Louise |
author_facet |
Imazaki, Pedro Henrique Brose, François Jauniaux, Thierry Das, Krishna Muller, Marc Scippo, Marie-Louise |
author_sort |
Imazaki, Pedro Henrique |
title |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_short |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_full |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_fullStr |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_full_unstemmed |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_sort |
estrogenic evaluation and organochlorine identification in blubber of north sea harbour porpoise (phocoena phocoena) stranded on the north sea coast |
publisher |
Hindawi Publishing Corporation |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449880/ http://www.ncbi.nlm.nih.gov/pubmed/26075240 https://doi.org/10.1155/2015/438295 |
genre |
Harbour porpoise Phocoena phocoena |
genre_facet |
Harbour porpoise Phocoena phocoena |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449880/ http://www.ncbi.nlm.nih.gov/pubmed/26075240 http://dx.doi.org/10.1155/2015/438295 |
op_rights |
Copyright © 2015 Pedro Henrique Imazaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1155/2015/438295 |
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BioMed Research International |
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2015 |
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1 |
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13 |
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1766023126068494336 |