Diversity and Dynamics of a North Atlantic Coastal Vibrio Community
Vibrios are ubiquitous marine bacteria that have long served as models for heterotrophic processes and have received renewed attention because of the discovery of increasing numbers of facultatively pathogenic strains. Because the occurrence of specific vibrios has frequently been linked to the temp...
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ftpubmed:oai:pubmedcentral.nih.gov:444776 2023-05-15T17:34:21+02:00 Diversity and Dynamics of a North Atlantic Coastal Vibrio Community Thompson, Janelle R. Randa, Mark A. Marcelino, Luisa A. Tomita-Mitchell, Aoy Lim, Eelin Polz, Martin F. 2004-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC444776 http://www.ncbi.nlm.nih.gov/pubmed/15240289 https://doi.org/10.1128/AEM.70.7.4103-4110.2004 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC444776 http://www.ncbi.nlm.nih.gov/pubmed/15240289 http://dx.doi.org/10.1128/AEM.70.7.4103-4110.2004 Copyright © 2004, American Society for Microbiology Microbial Ecology Text 2004 ftpubmed https://doi.org/10.1128/AEM.70.7.4103-4110.2004 2013-08-30T00:43:48Z Vibrios are ubiquitous marine bacteria that have long served as models for heterotrophic processes and have received renewed attention because of the discovery of increasing numbers of facultatively pathogenic strains. Because the occurrence of specific vibrios has frequently been linked to the temperature, salinity, and nutrient status of water, we hypothesized that seasonal changes in coastal water bodies lead to distinct vibrio communities and sought to characterize their level of differentiation. A novel technique was used to quantify shifts in 16S rRNA gene abundance in samples from Barnegat Bay, N.J., collected over a 15-month period. Quantitative PCR (QPCR) with primers specific for the genus Vibrio was combined with separation and quantification of amplicons by constant denaturant capillary electrophoresis (CDCE). Vibrio populations identified by QPCR-CDCE varied between summer and winter samples, suggesting distinct warm-water and year-round populations. Identification of the CDCE populations by cloning and sequencing of 16S rRNA genes from two summer and two winter samples confirmed this distinction. It further showed that CDCE populations corresponded in most cases to ∼98% rRNA similarity groups and suggested that the abundance of these follows temperature trends. Phylogenetic comparison yielded closely related cultured and often pathogenic representatives for most sequences, and the temperature ranges of these isolates confirmed the trends seen in the environmental samples. Overall, this suggests that temperature is a good predictor of the occurrence of closely related vibrios but that considerable microdiversity of unknown significance coexists within this trend. Text North Atlantic PubMed Central (PMC) Applied and Environmental Microbiology 70 7 4103 4110 |
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language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Thompson, Janelle R. Randa, Mark A. Marcelino, Luisa A. Tomita-Mitchell, Aoy Lim, Eelin Polz, Martin F. Diversity and Dynamics of a North Atlantic Coastal Vibrio Community |
topic_facet |
Microbial Ecology |
description |
Vibrios are ubiquitous marine bacteria that have long served as models for heterotrophic processes and have received renewed attention because of the discovery of increasing numbers of facultatively pathogenic strains. Because the occurrence of specific vibrios has frequently been linked to the temperature, salinity, and nutrient status of water, we hypothesized that seasonal changes in coastal water bodies lead to distinct vibrio communities and sought to characterize their level of differentiation. A novel technique was used to quantify shifts in 16S rRNA gene abundance in samples from Barnegat Bay, N.J., collected over a 15-month period. Quantitative PCR (QPCR) with primers specific for the genus Vibrio was combined with separation and quantification of amplicons by constant denaturant capillary electrophoresis (CDCE). Vibrio populations identified by QPCR-CDCE varied between summer and winter samples, suggesting distinct warm-water and year-round populations. Identification of the CDCE populations by cloning and sequencing of 16S rRNA genes from two summer and two winter samples confirmed this distinction. It further showed that CDCE populations corresponded in most cases to ∼98% rRNA similarity groups and suggested that the abundance of these follows temperature trends. Phylogenetic comparison yielded closely related cultured and often pathogenic representatives for most sequences, and the temperature ranges of these isolates confirmed the trends seen in the environmental samples. Overall, this suggests that temperature is a good predictor of the occurrence of closely related vibrios but that considerable microdiversity of unknown significance coexists within this trend. |
format |
Text |
author |
Thompson, Janelle R. Randa, Mark A. Marcelino, Luisa A. Tomita-Mitchell, Aoy Lim, Eelin Polz, Martin F. |
author_facet |
Thompson, Janelle R. Randa, Mark A. Marcelino, Luisa A. Tomita-Mitchell, Aoy Lim, Eelin Polz, Martin F. |
author_sort |
Thompson, Janelle R. |
title |
Diversity and Dynamics of a North Atlantic Coastal Vibrio Community |
title_short |
Diversity and Dynamics of a North Atlantic Coastal Vibrio Community |
title_full |
Diversity and Dynamics of a North Atlantic Coastal Vibrio Community |
title_fullStr |
Diversity and Dynamics of a North Atlantic Coastal Vibrio Community |
title_full_unstemmed |
Diversity and Dynamics of a North Atlantic Coastal Vibrio Community |
title_sort |
diversity and dynamics of a north atlantic coastal vibrio community |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC444776 http://www.ncbi.nlm.nih.gov/pubmed/15240289 https://doi.org/10.1128/AEM.70.7.4103-4110.2004 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC444776 http://www.ncbi.nlm.nih.gov/pubmed/15240289 http://dx.doi.org/10.1128/AEM.70.7.4103-4110.2004 |
op_rights |
Copyright © 2004, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.70.7.4103-4110.2004 |
container_title |
Applied and Environmental Microbiology |
container_volume |
70 |
container_issue |
7 |
container_start_page |
4103 |
op_container_end_page |
4110 |
_version_ |
1766133147867545600 |