Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis
Short Sequence Repeat (SSR) typing of Mycobacterium avium subspecies paratuberculosis (Map) isolates is one of the most commonly used method for genotyping this pathogen. Currently used techniques have challenges in analyzing mononucleotide repeats >15 bp, which include some of the Map SSRs. Frag...
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ftpubmed:oai:pubmedcentral.nih.gov:4415927 2023-05-15T17:19:50+02:00 Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis Podder, Milka P. Banfield, Susan E. Keefe, Greg P. Whitney, Hugh G. Tahlan, Kapil 2015-04-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415927/ http://www.ncbi.nlm.nih.gov/pubmed/25927612 https://doi.org/10.1371/journal.pone.0126071 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415927/ http://www.ncbi.nlm.nih.gov/pubmed/25927612 http://dx.doi.org/10.1371/journal.pone.0126071 http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0126071 2015-05-10T00:09:35Z Short Sequence Repeat (SSR) typing of Mycobacterium avium subspecies paratuberculosis (Map) isolates is one of the most commonly used method for genotyping this pathogen. Currently used techniques have challenges in analyzing mononucleotide repeats >15 bp, which include some of the Map SSRs. Fragment analysis is a relatively simple technique, which can accurately measure the size of DNA fragments and can be used to calculate the repeat length of the target SSR loci. In the present study, fragment analysis was used to analyze 4 Map SSR loci known to provide sufficient discriminatory power to determine the relationship between Map isolates. Eighty-five Map isolates from 18 animals from the island of Newfoundland were successfully genotyped using fragment analysis. To the best of our knowledge, this is the first report on Map SSR diversity from Newfoundland dairy farms. Previously unreported Map SSR-types or combinations were also identified during the course of the described work. In addition, multiple Map SSR-types were isolated from a single animal in many cases, which is not a common finding. Text Newfoundland PubMed Central (PMC) PLOS ONE 10 4 e0126071 |
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Research Article Podder, Milka P. Banfield, Susan E. Keefe, Greg P. Whitney, Hugh G. Tahlan, Kapil Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis |
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Research Article |
description |
Short Sequence Repeat (SSR) typing of Mycobacterium avium subspecies paratuberculosis (Map) isolates is one of the most commonly used method for genotyping this pathogen. Currently used techniques have challenges in analyzing mononucleotide repeats >15 bp, which include some of the Map SSRs. Fragment analysis is a relatively simple technique, which can accurately measure the size of DNA fragments and can be used to calculate the repeat length of the target SSR loci. In the present study, fragment analysis was used to analyze 4 Map SSR loci known to provide sufficient discriminatory power to determine the relationship between Map isolates. Eighty-five Map isolates from 18 animals from the island of Newfoundland were successfully genotyped using fragment analysis. To the best of our knowledge, this is the first report on Map SSR diversity from Newfoundland dairy farms. Previously unreported Map SSR-types or combinations were also identified during the course of the described work. In addition, multiple Map SSR-types were isolated from a single animal in many cases, which is not a common finding. |
format |
Text |
author |
Podder, Milka P. Banfield, Susan E. Keefe, Greg P. Whitney, Hugh G. Tahlan, Kapil |
author_facet |
Podder, Milka P. Banfield, Susan E. Keefe, Greg P. Whitney, Hugh G. Tahlan, Kapil |
author_sort |
Podder, Milka P. |
title |
Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis |
title_short |
Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis |
title_full |
Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis |
title_fullStr |
Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis |
title_full_unstemmed |
Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis |
title_sort |
typing of mycobacterium avium subspecies paratuberculosis isolates from newfoundland using fragment analysis |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415927/ http://www.ncbi.nlm.nih.gov/pubmed/25927612 https://doi.org/10.1371/journal.pone.0126071 |
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Newfoundland |
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Newfoundland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415927/ http://www.ncbi.nlm.nih.gov/pubmed/25927612 http://dx.doi.org/10.1371/journal.pone.0126071 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0126071 |
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PLOS ONE |
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10 |
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4 |
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e0126071 |
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