Detection of Angiostrongylus cantonensis in the Blood and Peripheral Tissues of Wild Hawaiian Rats (Rattus rattus) by a Quantitative PCR (qPCR) Assay
The nematode Angiostrongylus cantonensis is a rat lungworm, a zoonotic pathogen that causes human eosinophilic meningitis and ocular angiostrongyliasis characteristic of rat lungworm (RLW) disease. Definitive diagnosis is made by finding and identifying A. cantonensis larvae in the cerebral spinal f...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409314/ http://www.ncbi.nlm.nih.gov/pubmed/25910229 https://doi.org/10.1371/journal.pone.0123064 |
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ftpubmed:oai:pubmedcentral.nih.gov:4409314 2023-05-15T18:05:38+02:00 Detection of Angiostrongylus cantonensis in the Blood and Peripheral Tissues of Wild Hawaiian Rats (Rattus rattus) by a Quantitative PCR (qPCR) Assay Jarvi, Susan I. Pitt, William C. Farias, Margaret E. Shiels, Laura Severino, Michael G. Howe, Kathleen M. Jacquier, Steven H. Shiels, Aaron B. Amano, Karis K. Luiz, Blaine C. Maher, Daisy E. Allison, Maureen L. Holtquist, Zachariah C. Scheibelhut, Neil T. 2015-04-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409314/ http://www.ncbi.nlm.nih.gov/pubmed/25910229 https://doi.org/10.1371/journal.pone.0123064 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409314/ http://www.ncbi.nlm.nih.gov/pubmed/25910229 http://dx.doi.org/10.1371/journal.pone.0123064 https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication CC0 PDM Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0123064 2015-05-17T00:00:57Z The nematode Angiostrongylus cantonensis is a rat lungworm, a zoonotic pathogen that causes human eosinophilic meningitis and ocular angiostrongyliasis characteristic of rat lungworm (RLW) disease. Definitive diagnosis is made by finding and identifying A. cantonensis larvae in the cerebral spinal fluid or by using a custom immunological or molecular test. This study was conducted to determine if genomic DNA from A. cantonensis is detectable by qPCR in the blood or tissues of experimentally infected rats. F1 offspring from wild rats were subjected to experimental infection with RLW larvae isolated from slugs, then blood or tissue samples were collected over multiple time points. Blood samples were collected from 21 rats throughout the course of two trials (15 rats in Trial I, and 6 rats in Trial II). In addition to a control group, each trial had two treatment groups: the rats in the low dose (LD) group were infected by approximately 10 larvae and the rats in the high dose (HD) group were infected with approximately 50 larvae. In Trial I, parasite DNA was detected in cardiac bleed samples from five of five LD rats and five of five HD rats at six weeks post-infection (PI), and three of five LD rats and five of five HD rats from tail tissue. In Trial II, parasite DNA was detected in peripheral blood samples from one of two HD rats at 53 minutes PI, one of two LD rats at 1.5 hours PI, one of two HD rats at 18 hours PI, one of two LD rats at five weeks PI and two of two at six weeks PI, and two of two HD rats at weeks five and six PI. These data demonstrate that parasite DNA can be detected in peripheral blood at various time points throughout RLW infection in rats. Text Rattus rattus PubMed Central (PMC) PLOS ONE 10 4 e0123064 |
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Research Article Jarvi, Susan I. Pitt, William C. Farias, Margaret E. Shiels, Laura Severino, Michael G. Howe, Kathleen M. Jacquier, Steven H. Shiels, Aaron B. Amano, Karis K. Luiz, Blaine C. Maher, Daisy E. Allison, Maureen L. Holtquist, Zachariah C. Scheibelhut, Neil T. Detection of Angiostrongylus cantonensis in the Blood and Peripheral Tissues of Wild Hawaiian Rats (Rattus rattus) by a Quantitative PCR (qPCR) Assay |
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Research Article |
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The nematode Angiostrongylus cantonensis is a rat lungworm, a zoonotic pathogen that causes human eosinophilic meningitis and ocular angiostrongyliasis characteristic of rat lungworm (RLW) disease. Definitive diagnosis is made by finding and identifying A. cantonensis larvae in the cerebral spinal fluid or by using a custom immunological or molecular test. This study was conducted to determine if genomic DNA from A. cantonensis is detectable by qPCR in the blood or tissues of experimentally infected rats. F1 offspring from wild rats were subjected to experimental infection with RLW larvae isolated from slugs, then blood or tissue samples were collected over multiple time points. Blood samples were collected from 21 rats throughout the course of two trials (15 rats in Trial I, and 6 rats in Trial II). In addition to a control group, each trial had two treatment groups: the rats in the low dose (LD) group were infected by approximately 10 larvae and the rats in the high dose (HD) group were infected with approximately 50 larvae. In Trial I, parasite DNA was detected in cardiac bleed samples from five of five LD rats and five of five HD rats at six weeks post-infection (PI), and three of five LD rats and five of five HD rats from tail tissue. In Trial II, parasite DNA was detected in peripheral blood samples from one of two HD rats at 53 minutes PI, one of two LD rats at 1.5 hours PI, one of two HD rats at 18 hours PI, one of two LD rats at five weeks PI and two of two at six weeks PI, and two of two HD rats at weeks five and six PI. These data demonstrate that parasite DNA can be detected in peripheral blood at various time points throughout RLW infection in rats. |
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Text |
author |
Jarvi, Susan I. Pitt, William C. Farias, Margaret E. Shiels, Laura Severino, Michael G. Howe, Kathleen M. Jacquier, Steven H. Shiels, Aaron B. Amano, Karis K. Luiz, Blaine C. Maher, Daisy E. Allison, Maureen L. Holtquist, Zachariah C. Scheibelhut, Neil T. |
author_facet |
Jarvi, Susan I. Pitt, William C. Farias, Margaret E. Shiels, Laura Severino, Michael G. Howe, Kathleen M. Jacquier, Steven H. Shiels, Aaron B. Amano, Karis K. Luiz, Blaine C. Maher, Daisy E. Allison, Maureen L. Holtquist, Zachariah C. Scheibelhut, Neil T. |
author_sort |
Jarvi, Susan I. |
title |
Detection of Angiostrongylus cantonensis in the Blood and Peripheral Tissues of Wild Hawaiian Rats (Rattus rattus) by a Quantitative PCR (qPCR) Assay |
title_short |
Detection of Angiostrongylus cantonensis in the Blood and Peripheral Tissues of Wild Hawaiian Rats (Rattus rattus) by a Quantitative PCR (qPCR) Assay |
title_full |
Detection of Angiostrongylus cantonensis in the Blood and Peripheral Tissues of Wild Hawaiian Rats (Rattus rattus) by a Quantitative PCR (qPCR) Assay |
title_fullStr |
Detection of Angiostrongylus cantonensis in the Blood and Peripheral Tissues of Wild Hawaiian Rats (Rattus rattus) by a Quantitative PCR (qPCR) Assay |
title_full_unstemmed |
Detection of Angiostrongylus cantonensis in the Blood and Peripheral Tissues of Wild Hawaiian Rats (Rattus rattus) by a Quantitative PCR (qPCR) Assay |
title_sort |
detection of angiostrongylus cantonensis in the blood and peripheral tissues of wild hawaiian rats (rattus rattus) by a quantitative pcr (qpcr) assay |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409314/ http://www.ncbi.nlm.nih.gov/pubmed/25910229 https://doi.org/10.1371/journal.pone.0123064 |
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Rattus rattus |
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Rattus rattus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409314/ http://www.ncbi.nlm.nih.gov/pubmed/25910229 http://dx.doi.org/10.1371/journal.pone.0123064 |
op_rights |
https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication |
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CC0 PDM |
op_doi |
https://doi.org/10.1371/journal.pone.0123064 |
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PLOS ONE |
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10 |
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4 |
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e0123064 |
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