A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining
The fungus Pseudozyma antarctica produces a lipase (CalB) with broad substrate specificity, stability, high regio- and enantio-selectivity. It is active in non-aqueous organic solvents and at elevated temperatures. Hence, CalB is a robust biocatalyst for chemical conversions on an industrial scale....
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ftpubmed:oai:pubmedcentral.nih.gov:4405274 2023-05-15T13:44:46+02:00 A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining Vaquero, Maria E. de Eugenio, Laura I. Martínez, Maria J. Barriuso, Jorge 2015-04-21 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405274/ http://www.ncbi.nlm.nih.gov/pubmed/25898146 https://doi.org/10.1371/journal.pone.0124882 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405274/ http://www.ncbi.nlm.nih.gov/pubmed/25898146 http://dx.doi.org/10.1371/journal.pone.0124882 http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0124882 2015-05-10T00:01:34Z The fungus Pseudozyma antarctica produces a lipase (CalB) with broad substrate specificity, stability, high regio- and enantio-selectivity. It is active in non-aqueous organic solvents and at elevated temperatures. Hence, CalB is a robust biocatalyst for chemical conversions on an industrial scale. Here we report the in silico mining of public metagenomes and fungal genomes to discover novel lipases with high homology to CalB. The candidates were selected taking into account homology and conserved motifs criteria, as well as, phylogeny and 3D model analyses. The most promising candidate (PlicB) presented interesting structural properties. PlicB was expressed in a heterologous host, purified and partially characterized. Further experiments will allow finding novel catalytic properties with biotechnological interest. Text Antarc* Antarctica PubMed Central (PMC) PLOS ONE 10 4 e0124882 |
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Research Article Vaquero, Maria E. de Eugenio, Laura I. Martínez, Maria J. Barriuso, Jorge A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining |
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description |
The fungus Pseudozyma antarctica produces a lipase (CalB) with broad substrate specificity, stability, high regio- and enantio-selectivity. It is active in non-aqueous organic solvents and at elevated temperatures. Hence, CalB is a robust biocatalyst for chemical conversions on an industrial scale. Here we report the in silico mining of public metagenomes and fungal genomes to discover novel lipases with high homology to CalB. The candidates were selected taking into account homology and conserved motifs criteria, as well as, phylogeny and 3D model analyses. The most promising candidate (PlicB) presented interesting structural properties. PlicB was expressed in a heterologous host, purified and partially characterized. Further experiments will allow finding novel catalytic properties with biotechnological interest. |
format |
Text |
author |
Vaquero, Maria E. de Eugenio, Laura I. Martínez, Maria J. Barriuso, Jorge |
author_facet |
Vaquero, Maria E. de Eugenio, Laura I. Martínez, Maria J. Barriuso, Jorge |
author_sort |
Vaquero, Maria E. |
title |
A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining |
title_short |
A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining |
title_full |
A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining |
title_fullStr |
A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining |
title_full_unstemmed |
A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining |
title_sort |
novel calb-type lipase discovered by fungal genomes mining |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405274/ http://www.ncbi.nlm.nih.gov/pubmed/25898146 https://doi.org/10.1371/journal.pone.0124882 |
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Antarc* Antarctica |
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Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405274/ http://www.ncbi.nlm.nih.gov/pubmed/25898146 http://dx.doi.org/10.1371/journal.pone.0124882 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0124882 |
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PLOS ONE |
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10 |
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4 |
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e0124882 |
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