Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae)
Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic mal...
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ftpubmed:oai:pubmedcentral.nih.gov:4387377 2023-05-15T13:49:18+02:00 Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) Cocca, Ennio Petraccioli, Agnese Morescalchi, Maria Alessandra Odierna, Gaetano Capriglione, Teresa 2015-02-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387377 https://doi.org/10.3897/CompCytogen.v9i1.8731 en eng Pensoft Publishers http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://dx.doi.org/10.3897/CompCytogen.v9i1.8731 Ennio Cocca, Agnese Petraccioli, Maria Alessandra Morescalchi, Gaetano Odierna, Teresa Capriglione http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Articles Text 2015 ftpubmed https://doi.org/10.3897/CompCytogen.v9i1.8731 2015-04-19T00:01:05Z Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic males showing an X1YX2 karyotype and females an X1X1X2X2 karyotype. Fluorescence in situ hybridization, performed with a painting probe made from microdissected Y chromosomes, allowed a deeper insight on the chromosomal rearrangement, which underpinned the fusion event that generated the Y. Then, we used a DNA library established by microdissection and microcloning of the whole Y chromosome of Chionodraco hamatus for searching sex-linked sequences. One clone provided preliminary information on the presence on the Y chromosome of the CHD1 gene homologue, which is sex-linked in birds but in no other vertebrates. Several clones from the Y-chromosome mini-library contained microsatellites and transposable elements, one of which mapped to the q arm putative fusion region of the Y chromosome. The findings confirm that interspersed repetitive sequences might have fostered chromosome rearrangements and the emergence of the Y chromosome in Chionodraco hamatus. Detection of the CHD1 gene in the Y sex-determining region could be a classical example of convergent evolution in action. Text Antarc* Antarctic Southern Ocean PubMed Central (PMC) Antarctic Southern Ocean The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) The Antarctic Comparative Cytogenetics 9 1 1 15 |
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Research Articles Cocca, Ennio Petraccioli, Agnese Morescalchi, Maria Alessandra Odierna, Gaetano Capriglione, Teresa Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
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Research Articles |
description |
Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic males showing an X1YX2 karyotype and females an X1X1X2X2 karyotype. Fluorescence in situ hybridization, performed with a painting probe made from microdissected Y chromosomes, allowed a deeper insight on the chromosomal rearrangement, which underpinned the fusion event that generated the Y. Then, we used a DNA library established by microdissection and microcloning of the whole Y chromosome of Chionodraco hamatus for searching sex-linked sequences. One clone provided preliminary information on the presence on the Y chromosome of the CHD1 gene homologue, which is sex-linked in birds but in no other vertebrates. Several clones from the Y-chromosome mini-library contained microsatellites and transposable elements, one of which mapped to the q arm putative fusion region of the Y chromosome. The findings confirm that interspersed repetitive sequences might have fostered chromosome rearrangements and the emergence of the Y chromosome in Chionodraco hamatus. Detection of the CHD1 gene in the Y sex-determining region could be a classical example of convergent evolution in action. |
format |
Text |
author |
Cocca, Ennio Petraccioli, Agnese Morescalchi, Maria Alessandra Odierna, Gaetano Capriglione, Teresa |
author_facet |
Cocca, Ennio Petraccioli, Agnese Morescalchi, Maria Alessandra Odierna, Gaetano Capriglione, Teresa |
author_sort |
Cocca, Ennio |
title |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_short |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_full |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_fullStr |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_full_unstemmed |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_sort |
laser microdissection-based analysis of the y sex chromosome of the antarctic fish chionodraco hamatus (notothenioidei, channichthyidae) |
publisher |
Pensoft Publishers |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387377 https://doi.org/10.3897/CompCytogen.v9i1.8731 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
Antarctic Southern Ocean The ''Y'' The Antarctic |
geographic_facet |
Antarctic Southern Ocean The ''Y'' The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://dx.doi.org/10.3897/CompCytogen.v9i1.8731 |
op_rights |
Ennio Cocca, Agnese Petraccioli, Maria Alessandra Morescalchi, Gaetano Odierna, Teresa Capriglione http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3897/CompCytogen.v9i1.8731 |
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Comparative Cytogenetics |
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9 |
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1 |
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15 |
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1766251145288744960 |