Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3
Pseudoalteromonas antarctica NF3 is an Antarctic psychrotolerant Gram-negative bacterium that accumulates large amounts of an extracellular polymeric substance (EPS) with high protein content. Transmission electron microscopy analysis after high-pressure freezing and freeze substitution (HPF-FS) sho...
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ftpubmed:oai:pubmedcentral.nih.gov:4379500 2023-05-15T14:00:49+02:00 Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3 Nevot, Maria Deroncelé, Víctor Messner, Paul Guinea, Jesús Mercadé, Elena 2006-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379500 http://www.ncbi.nlm.nih.gov/pubmed/16913913 https://doi.org/10.1111/j.1462-2920.2006.01043.x en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/16913913 http://dx.doi.org/10.1111/j.1462-2920.2006.01043.x © 2006 The Authors Journal compilation © 2006 Society for Applied Microbiology and Blackwell Publishing Ltd Article Text 2006 ftpubmed https://doi.org/10.1111/j.1462-2920.2006.01043.x 2015-04-05T00:14:23Z Pseudoalteromonas antarctica NF3 is an Antarctic psychrotolerant Gram-negative bacterium that accumulates large amounts of an extracellular polymeric substance (EPS) with high protein content. Transmission electron microscopy analysis after high-pressure freezing and freeze substitution (HPF-FS) shows that the EPS is composed of a capsular polymer and large numbers of outer membrane vesicles (OMVs). These vesicles are bilayered structures and predominantly spherical in shape, with an average diameter of 25–70 nm, which is similar to what has been observed in OMVs from other Gram-negative bacteria. Analyses of lipopolysaccharide (LPS), phospholipids and protein profiles of OMVs are consistent with the bacterial outer membrane origin of these vesicles. In an initial attempt to elucidate the functions of OMVs proteins, we conducted a proteomic analysis on 1D SDS-PAGE bands. Those proteins putatively identified match with outer membrane proteins and proteins related to nutrient processing and transport in Gram-negative bacteria. This approach suggests that OMVs present in the EPS from P. antarctica NF3, might function to deliver proteins to the external media, and therefore play an important role in the survival of the bacterium in the extreme Antarctic environment. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic Environmental Microbiology 8 9 1523 1533 |
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Article Nevot, Maria Deroncelé, Víctor Messner, Paul Guinea, Jesús Mercadé, Elena Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3 |
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Article |
description |
Pseudoalteromonas antarctica NF3 is an Antarctic psychrotolerant Gram-negative bacterium that accumulates large amounts of an extracellular polymeric substance (EPS) with high protein content. Transmission electron microscopy analysis after high-pressure freezing and freeze substitution (HPF-FS) shows that the EPS is composed of a capsular polymer and large numbers of outer membrane vesicles (OMVs). These vesicles are bilayered structures and predominantly spherical in shape, with an average diameter of 25–70 nm, which is similar to what has been observed in OMVs from other Gram-negative bacteria. Analyses of lipopolysaccharide (LPS), phospholipids and protein profiles of OMVs are consistent with the bacterial outer membrane origin of these vesicles. In an initial attempt to elucidate the functions of OMVs proteins, we conducted a proteomic analysis on 1D SDS-PAGE bands. Those proteins putatively identified match with outer membrane proteins and proteins related to nutrient processing and transport in Gram-negative bacteria. This approach suggests that OMVs present in the EPS from P. antarctica NF3, might function to deliver proteins to the external media, and therefore play an important role in the survival of the bacterium in the extreme Antarctic environment. |
format |
Text |
author |
Nevot, Maria Deroncelé, Víctor Messner, Paul Guinea, Jesús Mercadé, Elena |
author_facet |
Nevot, Maria Deroncelé, Víctor Messner, Paul Guinea, Jesús Mercadé, Elena |
author_sort |
Nevot, Maria |
title |
Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3 |
title_short |
Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3 |
title_full |
Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3 |
title_fullStr |
Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3 |
title_full_unstemmed |
Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3 |
title_sort |
characterization of outer membrane vesicles released by the psychrotolerant bacterium pseudoalteromonas antarctica nf3 |
publishDate |
2006 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379500 http://www.ncbi.nlm.nih.gov/pubmed/16913913 https://doi.org/10.1111/j.1462-2920.2006.01043.x |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/16913913 http://dx.doi.org/10.1111/j.1462-2920.2006.01043.x |
op_rights |
© 2006 The Authors Journal compilation © 2006 Society for Applied Microbiology and Blackwell Publishing Ltd |
op_doi |
https://doi.org/10.1111/j.1462-2920.2006.01043.x |
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Environmental Microbiology |
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8 |
container_issue |
9 |
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1523 |
op_container_end_page |
1533 |
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1766270152895102976 |