Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris)
There are several reports of Sarcocystis sarcocysts in muscles of dogs but these species have not been named. Additionally, there are 2 reports of Sarcocystis neurona in dogs. Here, we propose 2 new names, Sarcocystis caninum, and Sarcocystis svanai for sarcocysts associated with clinical muscular s...
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ftpubmed:oai:pubmedcentral.nih.gov:4372507 2023-05-15T15:14:35+02:00 Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris) Dubey, J. P. Sykes, J. E. Shelton, G. D. Sharp, N. Verma, S. K. Calero-Bernal, R. Viviano, J. Sundar, N. Khan, A. Grigg, M. E. 2014-10-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372507/ http://www.ncbi.nlm.nih.gov/pubmed/25256157 https://doi.org/10.1111/jeu.12182 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372507/ http://www.ncbi.nlm.nih.gov/pubmed/25256157 http://dx.doi.org/10.1111/jeu.12182 Article Text 2014 ftpubmed https://doi.org/10.1111/jeu.12182 2016-05-08T00:01:36Z There are several reports of Sarcocystis sarcocysts in muscles of dogs but these species have not been named. Additionally, there are 2 reports of Sarcocystis neurona in dogs. Here, we propose 2 new names, Sarcocystis caninum, and Sarcocystis svanai for sarcocysts associated with clinical muscular sarcocystosis in 4 domestic dogs (Canis familiaris), 1 each from Montana and Colorado in the USA, and 2 from British Columbia, Canada. Only the sarcocyst stage was identified. Most of the sarcocysts identified were S. caninum. Sarcocysts were studied using light microscopy, transmission electron microscopy, and PCR. Based on collective results 2 new species, Sarcocystis caninum and Sarcocystis svanai were designated. Sarcocystis caninum and Sarcocystis svanai were structurally distinct. Sarcocystis caninum sarcocysts were up to 1.2 mm long and up to 75 μm wide. By light microscopy, the sarcocyst wall was relatively thin and smooth. By transmission electron microscopy (TEM), the sarcocyst wall “type 9”, 1–2 μm thick, and contained villar protrusions that lacked microtubules. Bradyzoites in sections were 7–9 μm long. Sarcocysts of S. svanai were few and were identified by TEM. Sarcocystis svanai sarcocysts were “type 1”, thin walled (< 0.5 μm), and the wall lacked villar protrusions but had tiny blebs that did not invaginate. DNA was extracted either from infected frozen muscle biopsies or formalin-fixed paraffin-embedded sections. Dogs were either singly infected with S. caninum or multiply co-infected with S. caninum and S. svanai (the result of a mixed infection) based on multi-locus DNA sequencing and morphology. BLASTn analysis established that the sarcocysts identified in these dogs were similar to, but not identical to S. canis or S. arctosi, parasites found to infect polar bears (Ursus maritimus) or brown bears (Ursus arctosi), respectively. However, the S. caninum sequence showed 100% identify over the 18S rRNA region sequenced to that of S. arctica, a parasite known to infect Arctic foxes (Vulpes lagopus). Text Arctic Ursus maritimus Vulpes lagopus PubMed Central (PMC) Arctic British Columbia ENVELOPE(-125.003,-125.003,54.000,54.000) Canada Journal of Eukaryotic Microbiology 62 3 307 317 |
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Article Dubey, J. P. Sykes, J. E. Shelton, G. D. Sharp, N. Verma, S. K. Calero-Bernal, R. Viviano, J. Sundar, N. Khan, A. Grigg, M. E. Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris) |
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description |
There are several reports of Sarcocystis sarcocysts in muscles of dogs but these species have not been named. Additionally, there are 2 reports of Sarcocystis neurona in dogs. Here, we propose 2 new names, Sarcocystis caninum, and Sarcocystis svanai for sarcocysts associated with clinical muscular sarcocystosis in 4 domestic dogs (Canis familiaris), 1 each from Montana and Colorado in the USA, and 2 from British Columbia, Canada. Only the sarcocyst stage was identified. Most of the sarcocysts identified were S. caninum. Sarcocysts were studied using light microscopy, transmission electron microscopy, and PCR. Based on collective results 2 new species, Sarcocystis caninum and Sarcocystis svanai were designated. Sarcocystis caninum and Sarcocystis svanai were structurally distinct. Sarcocystis caninum sarcocysts were up to 1.2 mm long and up to 75 μm wide. By light microscopy, the sarcocyst wall was relatively thin and smooth. By transmission electron microscopy (TEM), the sarcocyst wall “type 9”, 1–2 μm thick, and contained villar protrusions that lacked microtubules. Bradyzoites in sections were 7–9 μm long. Sarcocysts of S. svanai were few and were identified by TEM. Sarcocystis svanai sarcocysts were “type 1”, thin walled (< 0.5 μm), and the wall lacked villar protrusions but had tiny blebs that did not invaginate. DNA was extracted either from infected frozen muscle biopsies or formalin-fixed paraffin-embedded sections. Dogs were either singly infected with S. caninum or multiply co-infected with S. caninum and S. svanai (the result of a mixed infection) based on multi-locus DNA sequencing and morphology. BLASTn analysis established that the sarcocysts identified in these dogs were similar to, but not identical to S. canis or S. arctosi, parasites found to infect polar bears (Ursus maritimus) or brown bears (Ursus arctosi), respectively. However, the S. caninum sequence showed 100% identify over the 18S rRNA region sequenced to that of S. arctica, a parasite known to infect Arctic foxes (Vulpes lagopus). |
format |
Text |
author |
Dubey, J. P. Sykes, J. E. Shelton, G. D. Sharp, N. Verma, S. K. Calero-Bernal, R. Viviano, J. Sundar, N. Khan, A. Grigg, M. E. |
author_facet |
Dubey, J. P. Sykes, J. E. Shelton, G. D. Sharp, N. Verma, S. K. Calero-Bernal, R. Viviano, J. Sundar, N. Khan, A. Grigg, M. E. |
author_sort |
Dubey, J. P. |
title |
Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris) |
title_short |
Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris) |
title_full |
Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris) |
title_fullStr |
Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris) |
title_full_unstemmed |
Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris) |
title_sort |
sarcocystis caninum and sarcocystis svanai n. spp. (apicomplexa: sarcocystidae) associated with severe myositis and hepatitis in the domestic dog (canis familiaris) |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372507/ http://www.ncbi.nlm.nih.gov/pubmed/25256157 https://doi.org/10.1111/jeu.12182 |
long_lat |
ENVELOPE(-125.003,-125.003,54.000,54.000) |
geographic |
Arctic British Columbia Canada |
geographic_facet |
Arctic British Columbia Canada |
genre |
Arctic Ursus maritimus Vulpes lagopus |
genre_facet |
Arctic Ursus maritimus Vulpes lagopus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372507/ http://www.ncbi.nlm.nih.gov/pubmed/25256157 http://dx.doi.org/10.1111/jeu.12182 |
op_doi |
https://doi.org/10.1111/jeu.12182 |
container_title |
Journal of Eukaryotic Microbiology |
container_volume |
62 |
container_issue |
3 |
container_start_page |
307 |
op_container_end_page |
317 |
_version_ |
1766345022643372032 |