Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon
The aim of this study was to elucidate how vitamin E (alpha tocopherol) may ameliorate the toxicity of the pesticide chlorpyrifos in Atlantic salmon. Freshly isolated hepatocytes were exposed to vitamin E, chlorpyrifos or a combination of vitamin E and chlorpyrifos (all 100 μM). Transcriptomics (RNA...
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ftpubmed:oai:pubmedcentral.nih.gov:4361336 2023-05-15T15:30:46+02:00 Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon Olsvik, Pål A. Berntssen, Marc H. G. Søfteland, Liv 2015-03-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361336 http://www.ncbi.nlm.nih.gov/pubmed/25774794 https://doi.org/10.1371/journal.pone.0119250 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25774794 http://dx.doi.org/10.1371/journal.pone.0119250 http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0119250 2015-03-29T01:00:10Z The aim of this study was to elucidate how vitamin E (alpha tocopherol) may ameliorate the toxicity of the pesticide chlorpyrifos in Atlantic salmon. Freshly isolated hepatocytes were exposed to vitamin E, chlorpyrifos or a combination of vitamin E and chlorpyrifos (all 100 μM). Transcriptomics (RNA-seq) and metabolomics were used to screen for effects of vitamin E and chlorpyrifos. By introducing vitamin E, the number of upregulated transcripts induced by chlorpyrifos exposure was reduced from 941 to 626, while the number of downregulated transcripts was reduced from 901 to 742 compared to the control. Adding only vitamin E had no effect on the transcriptome. Jak-STAT signaling was the most significantly affected pathway by chlorpyrifos treatment according to the transcriptomics data. The metabolomics data showed that accumulation of multiple long chain fatty acids and dipeptides and amino acids in chlorpyrifos treated cells was partially alleviated by vitamin E treatment. Significant interaction effects between chlorpyrifos and vitamin E were seen for 15 metabolites, including 12 dipeptides. The antioxidant had relatively modest effects on chlorpyrifos-induced oxidative stress. By combining the two data sets, the study suggests that vitamin E supplementation prevents uptake and accumulation of fatty acids, and counteracts inhibited carbohydrate metabolism. Overall, this study shows that vitamin E only to a moderate degree modifies chlorpyrifos toxicity in Atlantic salmon liver cells. Text Atlantic salmon PubMed Central (PMC) PLOS ONE 10 3 e0119250 |
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Research Article Olsvik, Pål A. Berntssen, Marc H. G. Søfteland, Liv Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon |
topic_facet |
Research Article |
description |
The aim of this study was to elucidate how vitamin E (alpha tocopherol) may ameliorate the toxicity of the pesticide chlorpyrifos in Atlantic salmon. Freshly isolated hepatocytes were exposed to vitamin E, chlorpyrifos or a combination of vitamin E and chlorpyrifos (all 100 μM). Transcriptomics (RNA-seq) and metabolomics were used to screen for effects of vitamin E and chlorpyrifos. By introducing vitamin E, the number of upregulated transcripts induced by chlorpyrifos exposure was reduced from 941 to 626, while the number of downregulated transcripts was reduced from 901 to 742 compared to the control. Adding only vitamin E had no effect on the transcriptome. Jak-STAT signaling was the most significantly affected pathway by chlorpyrifos treatment according to the transcriptomics data. The metabolomics data showed that accumulation of multiple long chain fatty acids and dipeptides and amino acids in chlorpyrifos treated cells was partially alleviated by vitamin E treatment. Significant interaction effects between chlorpyrifos and vitamin E were seen for 15 metabolites, including 12 dipeptides. The antioxidant had relatively modest effects on chlorpyrifos-induced oxidative stress. By combining the two data sets, the study suggests that vitamin E supplementation prevents uptake and accumulation of fatty acids, and counteracts inhibited carbohydrate metabolism. Overall, this study shows that vitamin E only to a moderate degree modifies chlorpyrifos toxicity in Atlantic salmon liver cells. |
format |
Text |
author |
Olsvik, Pål A. Berntssen, Marc H. G. Søfteland, Liv |
author_facet |
Olsvik, Pål A. Berntssen, Marc H. G. Søfteland, Liv |
author_sort |
Olsvik, Pål A. |
title |
Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon |
title_short |
Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon |
title_full |
Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon |
title_fullStr |
Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon |
title_full_unstemmed |
Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon |
title_sort |
modifying effects of vitamin e on chlorpyrifos toxicity in atlantic salmon |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361336 http://www.ncbi.nlm.nih.gov/pubmed/25774794 https://doi.org/10.1371/journal.pone.0119250 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25774794 http://dx.doi.org/10.1371/journal.pone.0119250 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0119250 |
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PLOS ONE |
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10 |
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3 |
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e0119250 |
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