Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals*
Pharmaceuticals are pseudopersistent aquatic pollutants with unknown effects at environmentally relevant concentrations. Atlantic salmon (Salmo salar) were exposed to Acetaminophen: 54.77 ± 34.67; Atenolol: 11.08 ± 7.98, and Carbamazepine: 7.85 ± 0.13 μg·L−1 for 5 days. After Acetaminophen treatment...
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2015
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350032/ http://www.ncbi.nlm.nih.gov/pubmed/25394398 https://doi.org/10.1074/mcp.M114.045120 |
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ftpubmed:oai:pubmedcentral.nih.gov:4350032 2023-05-15T15:30:28+02:00 Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals* Hampel, Miriam Alonso, Esteban Aparicio, Irene Santos, Juan Luis Leaver, Michael 2015-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350032/ http://www.ncbi.nlm.nih.gov/pubmed/25394398 https://doi.org/10.1074/mcp.M114.045120 en eng The American Society for Biochemistry and Molecular Biology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350032/ http://www.ncbi.nlm.nih.gov/pubmed/25394398 http://dx.doi.org/10.1074/mcp.M114.045120 © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Research Text 2015 ftpubmed https://doi.org/10.1074/mcp.M114.045120 2016-02-07T01:12:37Z Pharmaceuticals are pseudopersistent aquatic pollutants with unknown effects at environmentally relevant concentrations. Atlantic salmon (Salmo salar) were exposed to Acetaminophen: 54.77 ± 34.67; Atenolol: 11.08 ± 7.98, and Carbamazepine: 7.85 ± 0.13 μg·L−1 for 5 days. After Acetaminophen treatment, 19 proteins were differently expressed, of which 11 were significant with respect to the control group (eight up-regulated and three down-regulated). After Atenolol treatment, seven differently expressed proteins were obtained in comparison with the control, of which six could be identified (four up-regulated and two down-regulated). Carbamazepine exposure resulted in 15 differently expressed proteins compared with the control, with 10 of them identified (seven up-regulated and three down-regulated). Out of these, three features were common between Acetaminophen and Carbamazepine and one between Carbamazepine and Atenolol. One feature was common across all treatments. Principal component analysis and heat map clustering showed a clear grouping of the variability caused by the applied treatments. The obtained data suggest (1) that exposure to environmentally relevant concentrations of the pharmaceuticals alters the hepatic protein expression profile of the Atlantic salmon; and (2) the existence of treatment specific processes that may be useful for biomarker development. Text Atlantic salmon Salmo salar PubMed Central (PMC) Molecular & Cellular Proteomics 14 2 371 381 |
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Research Hampel, Miriam Alonso, Esteban Aparicio, Irene Santos, Juan Luis Leaver, Michael Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals* |
topic_facet |
Research |
description |
Pharmaceuticals are pseudopersistent aquatic pollutants with unknown effects at environmentally relevant concentrations. Atlantic salmon (Salmo salar) were exposed to Acetaminophen: 54.77 ± 34.67; Atenolol: 11.08 ± 7.98, and Carbamazepine: 7.85 ± 0.13 μg·L−1 for 5 days. After Acetaminophen treatment, 19 proteins were differently expressed, of which 11 were significant with respect to the control group (eight up-regulated and three down-regulated). After Atenolol treatment, seven differently expressed proteins were obtained in comparison with the control, of which six could be identified (four up-regulated and two down-regulated). Carbamazepine exposure resulted in 15 differently expressed proteins compared with the control, with 10 of them identified (seven up-regulated and three down-regulated). Out of these, three features were common between Acetaminophen and Carbamazepine and one between Carbamazepine and Atenolol. One feature was common across all treatments. Principal component analysis and heat map clustering showed a clear grouping of the variability caused by the applied treatments. The obtained data suggest (1) that exposure to environmentally relevant concentrations of the pharmaceuticals alters the hepatic protein expression profile of the Atlantic salmon; and (2) the existence of treatment specific processes that may be useful for biomarker development. |
format |
Text |
author |
Hampel, Miriam Alonso, Esteban Aparicio, Irene Santos, Juan Luis Leaver, Michael |
author_facet |
Hampel, Miriam Alonso, Esteban Aparicio, Irene Santos, Juan Luis Leaver, Michael |
author_sort |
Hampel, Miriam |
title |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals* |
title_short |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals* |
title_full |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals* |
title_fullStr |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals* |
title_full_unstemmed |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals* |
title_sort |
hepatic proteome analysis of atlantic salmon (salmo salar) after exposure to environmental concentrations of human pharmaceuticals* |
publisher |
The American Society for Biochemistry and Molecular Biology |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350032/ http://www.ncbi.nlm.nih.gov/pubmed/25394398 https://doi.org/10.1074/mcp.M114.045120 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350032/ http://www.ncbi.nlm.nih.gov/pubmed/25394398 http://dx.doi.org/10.1074/mcp.M114.045120 |
op_rights |
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc. |
op_doi |
https://doi.org/10.1074/mcp.M114.045120 |
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Molecular & Cellular Proteomics |
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14 |
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2 |
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371 |
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381 |
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1766360915092963328 |