Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires
The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposur...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325608 http://www.ncbi.nlm.nih.gov/pubmed/25494467 https://doi.org/10.1590/0074-0276140258 |
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ftpubmed:oai:pubmedcentral.nih.gov:4325608 2023-05-15T15:50:28+02:00 Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires Millán, Javier García, Emilio J Oleaga, Álvaro López-Bao, José Vicente Llaneza, Luis Palacios, Vicente Candela, Mónica G Cevidanes, Aitor Rodríguez, Alejandro León-Vizcaíno, Luis 2014-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325608 http://www.ncbi.nlm.nih.gov/pubmed/25494467 https://doi.org/10.1590/0074-0276140258 en eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25494467 http://dx.doi.org/10.1590/0074-0276140258 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY-NC Articles Text 2014 ftpubmed https://doi.org/10.1590/0074-0276140258 2015-02-15T01:06:19Z The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposure of free-living wolves to 14 serovars of Leptospira interrogans sensu lato. Kidney samples from 49 wolves collected from 2010-2013 in northwestern Spain were analysed by culture, direct immunofluorescence and polymerase chain reaction. Tissue fluids were analysed for antibodies by a microscopic agglutination test. Ten wolves (observed prevalence: 20%, 95% confidence interval = 11-33%) showed evidence of contact with leptospires, eight through direct detection and nine through serology (7 wolves were positive according to both techniques). Titres below the cut-off level were also detected in seven cases. Serovars confirmed were Canicola (n = 4), Icterohaemorrhagiae (n = 3) and Sejroë, Ballum and Grippotyphosa (n = 1 each), indicating that wolves were infected with serovars for which dogs, rodents and ungulates, are the natural hosts and supporting the utility of the wolf and other large predators as environmental sentinels for pathogens. Text Canis lupus PubMed Central (PMC) Memórias do Instituto Oswaldo Cruz 109 8 1041 1044 |
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Articles Millán, Javier García, Emilio J Oleaga, Álvaro López-Bao, José Vicente Llaneza, Luis Palacios, Vicente Candela, Mónica G Cevidanes, Aitor Rodríguez, Alejandro León-Vizcaíno, Luis Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
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description |
The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposure of free-living wolves to 14 serovars of Leptospira interrogans sensu lato. Kidney samples from 49 wolves collected from 2010-2013 in northwestern Spain were analysed by culture, direct immunofluorescence and polymerase chain reaction. Tissue fluids were analysed for antibodies by a microscopic agglutination test. Ten wolves (observed prevalence: 20%, 95% confidence interval = 11-33%) showed evidence of contact with leptospires, eight through direct detection and nine through serology (7 wolves were positive according to both techniques). Titres below the cut-off level were also detected in seven cases. Serovars confirmed were Canicola (n = 4), Icterohaemorrhagiae (n = 3) and Sejroë, Ballum and Grippotyphosa (n = 1 each), indicating that wolves were infected with serovars for which dogs, rodents and ungulates, are the natural hosts and supporting the utility of the wolf and other large predators as environmental sentinels for pathogens. |
format |
Text |
author |
Millán, Javier García, Emilio J Oleaga, Álvaro López-Bao, José Vicente Llaneza, Luis Palacios, Vicente Candela, Mónica G Cevidanes, Aitor Rodríguez, Alejandro León-Vizcaíno, Luis |
author_facet |
Millán, Javier García, Emilio J Oleaga, Álvaro López-Bao, José Vicente Llaneza, Luis Palacios, Vicente Candela, Mónica G Cevidanes, Aitor Rodríguez, Alejandro León-Vizcaíno, Luis |
author_sort |
Millán, Javier |
title |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_short |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_full |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_fullStr |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_full_unstemmed |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_sort |
using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the iberian wolf and leptospires |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325608 http://www.ncbi.nlm.nih.gov/pubmed/25494467 https://doi.org/10.1590/0074-0276140258 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25494467 http://dx.doi.org/10.1590/0074-0276140258 |
op_rights |
http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1590/0074-0276140258 |
container_title |
Memórias do Instituto Oswaldo Cruz |
container_volume |
109 |
container_issue |
8 |
container_start_page |
1041 |
op_container_end_page |
1044 |
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1766385393861656576 |