A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species
There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped h...
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ftpubmed:oai:pubmedcentral.nih.gov:4315593 2023-05-15T15:53:51+02:00 A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species Fields, Andrew T. Abercrombie, Debra L. Eng, Rowena Feldheim, Kevin Chapman, Demian D. 2015-02-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315593 http://www.ncbi.nlm.nih.gov/pubmed/25646789 https://doi.org/10.1371/journal.pone.0114844 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25646789 http://dx.doi.org/10.1371/journal.pone.0114844 http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0114844 2015-02-15T00:58:47Z There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped hammerhead, Sphyrna lewini, smooth hammerhead, S. zygaena and great hammerhead S. mokarran) in addition to three species listed in the early part of this century (whale, Rhincodon typus, basking, Cetorhinus maximus, and white, Carcharodon carcharias). Shark fins are traded internationally to supply the Asian dried seafood market, in which they are used to make the luxury dish shark fin soup. Shark fins usually enter international trade with their skin still intact and can be identified using morphological characters or standard DNA-barcoding approaches. Once they reach Asia and are traded in this region the skin is removed and they are treated with chemicals that eliminate many key diagnostic characters and degrade their DNA (“processed fins”). Here, we present a validated mini-barcode assay based on partial sequences of the cytochrome oxidase I gene that can reliably identify the processed fins of seven of the eight CITES listed shark species. We also demonstrate that the assay can even frequently identify the species or genus of origin of shark fin soup (31 out of 50 samples). Text Cetorhinus maximus Lamna nasus Porbeagle PubMed Central (PMC) PLOS ONE 10 2 e0114844 |
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Research Article Fields, Andrew T. Abercrombie, Debra L. Eng, Rowena Feldheim, Kevin Chapman, Demian D. A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
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Research Article |
description |
There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped hammerhead, Sphyrna lewini, smooth hammerhead, S. zygaena and great hammerhead S. mokarran) in addition to three species listed in the early part of this century (whale, Rhincodon typus, basking, Cetorhinus maximus, and white, Carcharodon carcharias). Shark fins are traded internationally to supply the Asian dried seafood market, in which they are used to make the luxury dish shark fin soup. Shark fins usually enter international trade with their skin still intact and can be identified using morphological characters or standard DNA-barcoding approaches. Once they reach Asia and are traded in this region the skin is removed and they are treated with chemicals that eliminate many key diagnostic characters and degrade their DNA (“processed fins”). Here, we present a validated mini-barcode assay based on partial sequences of the cytochrome oxidase I gene that can reliably identify the processed fins of seven of the eight CITES listed shark species. We also demonstrate that the assay can even frequently identify the species or genus of origin of shark fin soup (31 out of 50 samples). |
format |
Text |
author |
Fields, Andrew T. Abercrombie, Debra L. Eng, Rowena Feldheim, Kevin Chapman, Demian D. |
author_facet |
Fields, Andrew T. Abercrombie, Debra L. Eng, Rowena Feldheim, Kevin Chapman, Demian D. |
author_sort |
Fields, Andrew T. |
title |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_short |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_full |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_fullStr |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_full_unstemmed |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_sort |
novel mini-dna barcoding assay to identify processed fins from internationally protected shark species |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315593 http://www.ncbi.nlm.nih.gov/pubmed/25646789 https://doi.org/10.1371/journal.pone.0114844 |
genre |
Cetorhinus maximus Lamna nasus Porbeagle |
genre_facet |
Cetorhinus maximus Lamna nasus Porbeagle |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25646789 http://dx.doi.org/10.1371/journal.pone.0114844 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0114844 |
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PLOS ONE |
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10 |
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2 |
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e0114844 |
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