Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria
Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles....
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ftpubmed:oai:pubmedcentral.nih.gov:4291224 2023-05-15T13:51:49+02:00 Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria Pérez-Cruz, Carla Delgado, Lidia López-Iglesias, Carmen Mercade, Elena 2015-01-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291224 http://www.ncbi.nlm.nih.gov/pubmed/25581302 https://doi.org/10.1371/journal.pone.0116896 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25581302 http://dx.doi.org/10.1371/journal.pone.0116896 http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0116896 2015-01-25T00:58:01Z Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicles. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic PLOS ONE 10 1 e0116896 |
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Research Article Pérez-Cruz, Carla Delgado, Lidia López-Iglesias, Carmen Mercade, Elena Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria |
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Research Article |
description |
Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicles. |
format |
Text |
author |
Pérez-Cruz, Carla Delgado, Lidia López-Iglesias, Carmen Mercade, Elena |
author_facet |
Pérez-Cruz, Carla Delgado, Lidia López-Iglesias, Carmen Mercade, Elena |
author_sort |
Pérez-Cruz, Carla |
title |
Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria |
title_short |
Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria |
title_full |
Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria |
title_fullStr |
Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria |
title_full_unstemmed |
Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria |
title_sort |
outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291224 http://www.ncbi.nlm.nih.gov/pubmed/25581302 https://doi.org/10.1371/journal.pone.0116896 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
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Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25581302 http://dx.doi.org/10.1371/journal.pone.0116896 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0116896 |
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PLOS ONE |
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10 |
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e0116896 |
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