In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr

The effect of serial in vitro subculturing on three pathogenic strains of Saprolegnia parasitica was investigated. The isolates were passed through Atlantic salmon, Salmo salar L. parr, and then re-isolated as single spore colonies. All strains caused infection. The isolate obtained from diseased fi...

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Published in:Journal of Fish Diseases
Main Authors: Songe, M M, Thoen, E, Evensen, Ø, Skaar, I
Format: Text
Language:English
Published: BlackWell Publishing Ltd 2014
Subjects:
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263310
http://www.ncbi.nlm.nih.gov/pubmed/24117449
https://doi.org/10.1111/jfd.12175
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spelling ftpubmed:oai:pubmedcentral.nih.gov:4263310 2023-05-15T15:30:35+02:00 In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr Songe, M M Thoen, E Evensen, Ø Skaar, I 2014-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263310 http://www.ncbi.nlm.nih.gov/pubmed/24117449 https://doi.org/10.1111/jfd.12175 en eng BlackWell Publishing Ltd http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24117449 http://dx.doi.org/10.1111/jfd.12175 © 2013 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. CC-BY-NC-ND Original Articles Text 2014 ftpubmed https://doi.org/10.1111/jfd.12175 2014-12-21T01:01:47Z The effect of serial in vitro subculturing on three pathogenic strains of Saprolegnia parasitica was investigated. The isolates were passed through Atlantic salmon, Salmo salar L. parr, and then re-isolated as single spore colonies. All strains caused infection. The isolate obtained from diseased fish served as a virulent reference culture and was designated ‘AP’ (‘activated through passage’). Successive subculturing was made by obtaining an inoculum from AP to produce the 2nd subculture and then passaged to the 3rd subculture (from the 2nd), until the 15th passage was obtained. Spores used to produce storage cultures were collected at passages 5, 10 and 15. The different passages of each strain were used to artificially infect Atlantic salmon parr. Morphological characterization of growth patterns was performed to observe differences occurring due to serial in vitro subculturing. Two of the strains declined in virulence after 15 successive in vitro subcultures, whereas one did not. This study is the first to investigate attenuation of virulence in Saprolegnia and whether or not isolates of S. parasitica should be passed through the fish host prior to challenge experiments. It reveals that some strains degenerate more rapidly than others when subjected to successive in vitro subculturing on glucose–yeast extract. Text Atlantic salmon Salmo salar PubMed Central (PMC) Journal of Fish Diseases 37 9 825 834
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Original Articles
spellingShingle Original Articles
Songe, M M
Thoen, E
Evensen, Ø
Skaar, I
In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr
topic_facet Original Articles
description The effect of serial in vitro subculturing on three pathogenic strains of Saprolegnia parasitica was investigated. The isolates were passed through Atlantic salmon, Salmo salar L. parr, and then re-isolated as single spore colonies. All strains caused infection. The isolate obtained from diseased fish served as a virulent reference culture and was designated ‘AP’ (‘activated through passage’). Successive subculturing was made by obtaining an inoculum from AP to produce the 2nd subculture and then passaged to the 3rd subculture (from the 2nd), until the 15th passage was obtained. Spores used to produce storage cultures were collected at passages 5, 10 and 15. The different passages of each strain were used to artificially infect Atlantic salmon parr. Morphological characterization of growth patterns was performed to observe differences occurring due to serial in vitro subculturing. Two of the strains declined in virulence after 15 successive in vitro subcultures, whereas one did not. This study is the first to investigate attenuation of virulence in Saprolegnia and whether or not isolates of S. parasitica should be passed through the fish host prior to challenge experiments. It reveals that some strains degenerate more rapidly than others when subjected to successive in vitro subculturing on glucose–yeast extract.
format Text
author Songe, M M
Thoen, E
Evensen, Ø
Skaar, I
author_facet Songe, M M
Thoen, E
Evensen, Ø
Skaar, I
author_sort Songe, M M
title In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr
title_short In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr
title_full In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr
title_fullStr In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr
title_full_unstemmed In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr
title_sort in vitro passages impact on virulence of saprolegnia parasitica to atlantic salmon, salmo salar l. parr
publisher BlackWell Publishing Ltd
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263310
http://www.ncbi.nlm.nih.gov/pubmed/24117449
https://doi.org/10.1111/jfd.12175
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC
http://www.ncbi.nlm.nih.gov/pubmed/24117449
http://dx.doi.org/10.1111/jfd.12175
op_rights © 2013 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd.
http://creativecommons.org/licenses/by-nc-nd/3.0/
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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container_title Journal of Fish Diseases
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