Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch
Knowledge of ancient viruses is limited due to their low concentration and poor preservation in ancient specimens. Using a viral particle-associated nucleic acid enrichment approach, we genetically characterized one complete DNA and one partial RNA viral genome from a 700-y-old fecal sample preserve...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250163/ http://www.ncbi.nlm.nih.gov/pubmed/25349412 https://doi.org/10.1073/pnas.1410429111 |
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ftpubmed:oai:pubmedcentral.nih.gov:4250163 2023-05-15T18:28:17+02:00 Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch Ng, Terry Fei Fan Chen, Li-Fang Zhou, Yanchen Shapiro, Beth Stiller, Mathias Heintzman, Peter D. Varsani, Arvind Kondov, Nikola O. Wong, Walt Deng, Xutao Andrews, Thomas D. Moorman, Brian J. Meulendyk, Thomas MacKay, Glen Gilbertson, Robert L. Delwart, Eric 2014-11-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250163/ http://www.ncbi.nlm.nih.gov/pubmed/25349412 https://doi.org/10.1073/pnas.1410429111 en eng National Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250163/ http://www.ncbi.nlm.nih.gov/pubmed/25349412 http://dx.doi.org/10.1073/pnas.1410429111 Biological Sciences Text 2014 ftpubmed https://doi.org/10.1073/pnas.1410429111 2015-05-30T23:58:31Z Knowledge of ancient viruses is limited due to their low concentration and poor preservation in ancient specimens. Using a viral particle-associated nucleic acid enrichment approach, we genetically characterized one complete DNA and one partial RNA viral genome from a 700-y-old fecal sample preserved in ice. Using reverse genetics, we reconstituted the DNA virus, which replicated and systemically spread in a model plant species. Under constant freezing conditions, encapsidated viral nucleic acids may therefore be preserved for centuries. Our finding indicates that cryogenically preserved materials can be repositories of ancient viral nucleic acids, which in turn allow molecular genetics to regenerate viruses to study their biology. Text Subarctic PubMed Central (PMC) Proceedings of the National Academy of Sciences 111 47 16842 16847 |
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Biological Sciences |
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Biological Sciences Ng, Terry Fei Fan Chen, Li-Fang Zhou, Yanchen Shapiro, Beth Stiller, Mathias Heintzman, Peter D. Varsani, Arvind Kondov, Nikola O. Wong, Walt Deng, Xutao Andrews, Thomas D. Moorman, Brian J. Meulendyk, Thomas MacKay, Glen Gilbertson, Robert L. Delwart, Eric Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch |
topic_facet |
Biological Sciences |
description |
Knowledge of ancient viruses is limited due to their low concentration and poor preservation in ancient specimens. Using a viral particle-associated nucleic acid enrichment approach, we genetically characterized one complete DNA and one partial RNA viral genome from a 700-y-old fecal sample preserved in ice. Using reverse genetics, we reconstituted the DNA virus, which replicated and systemically spread in a model plant species. Under constant freezing conditions, encapsidated viral nucleic acids may therefore be preserved for centuries. Our finding indicates that cryogenically preserved materials can be repositories of ancient viral nucleic acids, which in turn allow molecular genetics to regenerate viruses to study their biology. |
format |
Text |
author |
Ng, Terry Fei Fan Chen, Li-Fang Zhou, Yanchen Shapiro, Beth Stiller, Mathias Heintzman, Peter D. Varsani, Arvind Kondov, Nikola O. Wong, Walt Deng, Xutao Andrews, Thomas D. Moorman, Brian J. Meulendyk, Thomas MacKay, Glen Gilbertson, Robert L. Delwart, Eric |
author_facet |
Ng, Terry Fei Fan Chen, Li-Fang Zhou, Yanchen Shapiro, Beth Stiller, Mathias Heintzman, Peter D. Varsani, Arvind Kondov, Nikola O. Wong, Walt Deng, Xutao Andrews, Thomas D. Moorman, Brian J. Meulendyk, Thomas MacKay, Glen Gilbertson, Robert L. Delwart, Eric |
author_sort |
Ng, Terry Fei Fan |
title |
Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch |
title_short |
Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch |
title_full |
Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch |
title_fullStr |
Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch |
title_full_unstemmed |
Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch |
title_sort |
preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch |
publisher |
National Academy of Sciences |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250163/ http://www.ncbi.nlm.nih.gov/pubmed/25349412 https://doi.org/10.1073/pnas.1410429111 |
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Subarctic |
genre_facet |
Subarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250163/ http://www.ncbi.nlm.nih.gov/pubmed/25349412 http://dx.doi.org/10.1073/pnas.1410429111 |
op_doi |
https://doi.org/10.1073/pnas.1410429111 |
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Proceedings of the National Academy of Sciences |
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111 |
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47 |
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16842 |
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16847 |
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1766210683282653184 |