Transcriptome analyses of Anguillicola crassus from native and novel hosts
Anguillicola crassus is a swim bladder nematode of eels. The parasite is native to the Asian eel Anguilla japonica, but was introduced to Europe and the European eel Anguilla anguilla in the early 1980s. A Taiwanese source has been proposed for this introduction. In the new host in the recipient are...
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ftpubmed:oai:pubmedcentral.nih.gov:4250067 2023-05-15T13:27:57+02:00 Transcriptome analyses of Anguillicola crassus from native and novel hosts Heitlinger, Emanuel Taraschewski, Horst Weclawski, Urszula Gharbi, Karim Blaxter, Mark 2014-11-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250067 http://www.ncbi.nlm.nih.gov/pubmed/25469324 https://doi.org/10.7717/peerj.684 en eng PeerJ Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25469324 http://dx.doi.org/10.7717/peerj.684 © 2014 Heitlinger et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. CC-BY Evolutionary Studies Text 2014 ftpubmed https://doi.org/10.7717/peerj.684 2014-12-07T01:48:04Z Anguillicola crassus is a swim bladder nematode of eels. The parasite is native to the Asian eel Anguilla japonica, but was introduced to Europe and the European eel Anguilla anguilla in the early 1980s. A Taiwanese source has been proposed for this introduction. In the new host in the recipient area, the parasite appears to be more pathogenic. As a reason for these differences, genetically fixed differences in infectivity and development between Taiwanese and European A.crassus have been described and disentangled from plasticity induced by different host environments. To explore whether transcriptional regulation is involved in these lifecycle differences, we have analysed a “common garden”, cross infection experiment, using deep-sequencing transcriptomics. Surprisingly, in the face of clear phenotypic differences in life history traits, we identified no significant differences in gene expression between parasite populations or between experimental host species. From 120,000 SNPs identified in the transcriptome data we found that European A. crassus were not a genetic subset of the Taiwanese nematodes sampled. The loci that have the major contribution to the European-Taiwanese population differentiation show an enrichment of synonymous and non-coding polymorphism. This argues against positive selection in population differentiation. However, genes involved in protein processing in the endoplasmatic reticulum membrane and genes bearing secretion signal sequences were enriched in the set of genes most differentiated between European and Taiwanese A. crassus. These genes could be a source for the phenotypically visible genetically fixed differences between European and Taiwanese A. crassus. Text Anguilla anguilla European eel PubMed Central (PMC) PeerJ 2 e684 |
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Evolutionary Studies Heitlinger, Emanuel Taraschewski, Horst Weclawski, Urszula Gharbi, Karim Blaxter, Mark Transcriptome analyses of Anguillicola crassus from native and novel hosts |
topic_facet |
Evolutionary Studies |
description |
Anguillicola crassus is a swim bladder nematode of eels. The parasite is native to the Asian eel Anguilla japonica, but was introduced to Europe and the European eel Anguilla anguilla in the early 1980s. A Taiwanese source has been proposed for this introduction. In the new host in the recipient area, the parasite appears to be more pathogenic. As a reason for these differences, genetically fixed differences in infectivity and development between Taiwanese and European A.crassus have been described and disentangled from plasticity induced by different host environments. To explore whether transcriptional regulation is involved in these lifecycle differences, we have analysed a “common garden”, cross infection experiment, using deep-sequencing transcriptomics. Surprisingly, in the face of clear phenotypic differences in life history traits, we identified no significant differences in gene expression between parasite populations or between experimental host species. From 120,000 SNPs identified in the transcriptome data we found that European A. crassus were not a genetic subset of the Taiwanese nematodes sampled. The loci that have the major contribution to the European-Taiwanese population differentiation show an enrichment of synonymous and non-coding polymorphism. This argues against positive selection in population differentiation. However, genes involved in protein processing in the endoplasmatic reticulum membrane and genes bearing secretion signal sequences were enriched in the set of genes most differentiated between European and Taiwanese A. crassus. These genes could be a source for the phenotypically visible genetically fixed differences between European and Taiwanese A. crassus. |
format |
Text |
author |
Heitlinger, Emanuel Taraschewski, Horst Weclawski, Urszula Gharbi, Karim Blaxter, Mark |
author_facet |
Heitlinger, Emanuel Taraschewski, Horst Weclawski, Urszula Gharbi, Karim Blaxter, Mark |
author_sort |
Heitlinger, Emanuel |
title |
Transcriptome analyses of Anguillicola crassus from native and novel hosts |
title_short |
Transcriptome analyses of Anguillicola crassus from native and novel hosts |
title_full |
Transcriptome analyses of Anguillicola crassus from native and novel hosts |
title_fullStr |
Transcriptome analyses of Anguillicola crassus from native and novel hosts |
title_full_unstemmed |
Transcriptome analyses of Anguillicola crassus from native and novel hosts |
title_sort |
transcriptome analyses of anguillicola crassus from native and novel hosts |
publisher |
PeerJ Inc. |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250067 http://www.ncbi.nlm.nih.gov/pubmed/25469324 https://doi.org/10.7717/peerj.684 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25469324 http://dx.doi.org/10.7717/peerj.684 |
op_rights |
© 2014 Heitlinger et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.7717/peerj.684 |
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PeerJ |
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2 |
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e684 |
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