Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties
Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-fr...
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ftpubmed:oai:pubmedcentral.nih.gov:4249020 2023-05-15T13:55:25+02:00 Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties Pugin, Benoit Cornejo, Fabián A. Muñoz-Díaz, Pablo Muñoz-Villagrán, Claudia M. Vargas-Pérez, Joaquín I. Arenas, Felipe A. Vásquez, Claudio C. 2014-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249020/ http://www.ncbi.nlm.nih.gov/pubmed/25193000 https://doi.org/10.1128/AEM.02207-14 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249020/ http://www.ncbi.nlm.nih.gov/pubmed/25193000 http://dx.doi.org/10.1128/AEM.02207-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved. Environmental Microbiology Text 2014 ftpubmed https://doi.org/10.1128/AEM.02207-14 2015-05-03T00:11:07Z Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting approach compared to their chemical synthesis. To generate Te-containing nanostructures, bacteria enzymatically reduce tellurite to elemental tellurium. In this work, using a classic biochemical approach, we looked for a novel tellurite reductase from the Antarctic bacterium Pseudomonas sp. strain BNF22 and used it to generate tellurium-containing nanostructures. A new tellurite reductase was identified as glutathione reductase, which was subsequently overproduced in Escherichia coli. The characterization of this enzyme showed that it is an NADPH-dependent tellurite reductase, with optimum reducing activity at 30°C and pH 9.0. Finally, the enzyme was able to generate Te-containing nanostructures, about 68 nm in size, which exhibit interesting antibacterial properties against E. coli, with no apparent cytotoxicity against eukaryotic cells. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Applied and Environmental Microbiology 80 22 7061 7070 |
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Environmental Microbiology |
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Environmental Microbiology Pugin, Benoit Cornejo, Fabián A. Muñoz-Díaz, Pablo Muñoz-Villagrán, Claudia M. Vargas-Pérez, Joaquín I. Arenas, Felipe A. Vásquez, Claudio C. Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
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Environmental Microbiology |
description |
Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting approach compared to their chemical synthesis. To generate Te-containing nanostructures, bacteria enzymatically reduce tellurite to elemental tellurium. In this work, using a classic biochemical approach, we looked for a novel tellurite reductase from the Antarctic bacterium Pseudomonas sp. strain BNF22 and used it to generate tellurium-containing nanostructures. A new tellurite reductase was identified as glutathione reductase, which was subsequently overproduced in Escherichia coli. The characterization of this enzyme showed that it is an NADPH-dependent tellurite reductase, with optimum reducing activity at 30°C and pH 9.0. Finally, the enzyme was able to generate Te-containing nanostructures, about 68 nm in size, which exhibit interesting antibacterial properties against E. coli, with no apparent cytotoxicity against eukaryotic cells. |
format |
Text |
author |
Pugin, Benoit Cornejo, Fabián A. Muñoz-Díaz, Pablo Muñoz-Villagrán, Claudia M. Vargas-Pérez, Joaquín I. Arenas, Felipe A. Vásquez, Claudio C. |
author_facet |
Pugin, Benoit Cornejo, Fabián A. Muñoz-Díaz, Pablo Muñoz-Villagrán, Claudia M. Vargas-Pérez, Joaquín I. Arenas, Felipe A. Vásquez, Claudio C. |
author_sort |
Pugin, Benoit |
title |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_short |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_full |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_fullStr |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_full_unstemmed |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_sort |
glutathione reductase-mediated synthesis of tellurium-containing nanostructures exhibiting antibacterial properties |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249020/ http://www.ncbi.nlm.nih.gov/pubmed/25193000 https://doi.org/10.1128/AEM.02207-14 |
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Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249020/ http://www.ncbi.nlm.nih.gov/pubmed/25193000 http://dx.doi.org/10.1128/AEM.02207-14 |
op_rights |
Copyright © 2014, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.02207-14 |
container_title |
Applied and Environmental Microbiology |
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80 |
container_issue |
22 |
container_start_page |
7061 |
op_container_end_page |
7070 |
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1766262033613848576 |