Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes
Piscine orthoreovirus (PRV) belongs to the Reoviridae family and is the only known fish virus related to the Orthoreovirus genus. The virus is the causative agent of heart and skeletal muscle inflammation (HSMI), an emerging disease in farmed Atlantic salmon (Salmo salar L.). PRV is ubiquitous in fa...
Published in: | Veterinary Research |
---|---|
Main Authors: | , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
BioMed Central
2014
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234517 http://www.ncbi.nlm.nih.gov/pubmed/24694042 https://doi.org/10.1186/1297-9716-45-35 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:4234517 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:4234517 2023-05-15T15:30:46+02:00 Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes Finstad, Øystein Wessel Dahle, Maria Krudtaa Lindholm, Tone Hæg Nyman, Ingvild Berg Løvoll, Marie Wallace, Christian Olsen, Christel Moræus Storset, Anne K Rimstad, Espen 2014 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234517 http://www.ncbi.nlm.nih.gov/pubmed/24694042 https://doi.org/10.1186/1297-9716-45-35 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24694042 http://dx.doi.org/10.1186/1297-9716-45-35 Copyright © 2014 Finstad et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC-BY CC0 PDM Research Text 2014 ftpubmed https://doi.org/10.1186/1297-9716-45-35 2014-11-23T01:04:13Z Piscine orthoreovirus (PRV) belongs to the Reoviridae family and is the only known fish virus related to the Orthoreovirus genus. The virus is the causative agent of heart and skeletal muscle inflammation (HSMI), an emerging disease in farmed Atlantic salmon (Salmo salar L.). PRV is ubiquitous in farmed Atlantic salmon and high loads of PRV in the heart are consistent findings in HSMI. The mechanism by which PRV infection causes disease remains largely unknown. In this study we investigated the presence of PRV in blood and erythrocytes using an experimental cohabitation challenge model. We found that in the early phases of infection, the PRV loads in blood were significantly higher than in any other organ. Most virus was found in the erythrocyte fraction, and in individual fish more than 50% of erythrocytes were PRV-positive, as determined by flow cytometry. PRV was condensed into large cytoplasmic inclusions resembling viral factories, as demonstrated by immunofluorescence and confocal microscopy. By electron microscopy we showed that these inclusions contained reovirus-like particles. The PRV particles and inclusions also had a striking resemblance to previously reported viral inclusions described as Erythrocytic inclusion body syndrome (EIBS). We conclude that the erythrocyte is a major target cell for PRV infection. These findings provide new information about HSMI pathogenesis, and show that PRV is an important factor of viral erythrocytic inclusions. Text Atlantic salmon Salmo salar PubMed Central (PMC) Veterinary Research 45 1 35 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research |
spellingShingle |
Research Finstad, Øystein Wessel Dahle, Maria Krudtaa Lindholm, Tone Hæg Nyman, Ingvild Berg Løvoll, Marie Wallace, Christian Olsen, Christel Moræus Storset, Anne K Rimstad, Espen Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes |
topic_facet |
Research |
description |
Piscine orthoreovirus (PRV) belongs to the Reoviridae family and is the only known fish virus related to the Orthoreovirus genus. The virus is the causative agent of heart and skeletal muscle inflammation (HSMI), an emerging disease in farmed Atlantic salmon (Salmo salar L.). PRV is ubiquitous in farmed Atlantic salmon and high loads of PRV in the heart are consistent findings in HSMI. The mechanism by which PRV infection causes disease remains largely unknown. In this study we investigated the presence of PRV in blood and erythrocytes using an experimental cohabitation challenge model. We found that in the early phases of infection, the PRV loads in blood were significantly higher than in any other organ. Most virus was found in the erythrocyte fraction, and in individual fish more than 50% of erythrocytes were PRV-positive, as determined by flow cytometry. PRV was condensed into large cytoplasmic inclusions resembling viral factories, as demonstrated by immunofluorescence and confocal microscopy. By electron microscopy we showed that these inclusions contained reovirus-like particles. The PRV particles and inclusions also had a striking resemblance to previously reported viral inclusions described as Erythrocytic inclusion body syndrome (EIBS). We conclude that the erythrocyte is a major target cell for PRV infection. These findings provide new information about HSMI pathogenesis, and show that PRV is an important factor of viral erythrocytic inclusions. |
format |
Text |
author |
Finstad, Øystein Wessel Dahle, Maria Krudtaa Lindholm, Tone Hæg Nyman, Ingvild Berg Løvoll, Marie Wallace, Christian Olsen, Christel Moræus Storset, Anne K Rimstad, Espen |
author_facet |
Finstad, Øystein Wessel Dahle, Maria Krudtaa Lindholm, Tone Hæg Nyman, Ingvild Berg Løvoll, Marie Wallace, Christian Olsen, Christel Moræus Storset, Anne K Rimstad, Espen |
author_sort |
Finstad, Øystein Wessel |
title |
Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes |
title_short |
Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes |
title_full |
Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes |
title_fullStr |
Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes |
title_full_unstemmed |
Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes |
title_sort |
piscine orthoreovirus (prv) infects atlantic salmon erythrocytes |
publisher |
BioMed Central |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234517 http://www.ncbi.nlm.nih.gov/pubmed/24694042 https://doi.org/10.1186/1297-9716-45-35 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24694042 http://dx.doi.org/10.1186/1297-9716-45-35 |
op_rights |
Copyright © 2014 Finstad et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
op_rightsnorm |
CC-BY CC0 PDM |
op_doi |
https://doi.org/10.1186/1297-9716-45-35 |
container_title |
Veterinary Research |
container_volume |
45 |
container_issue |
1 |
container_start_page |
35 |
_version_ |
1766361215245746176 |