Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures
Cell cultures of trout gonad tissue (RTG-2) and Atlantic salmon heart, kidney, liver, and spleen tissue were inoculated with 50 50% tissue culture infective doses (TCID50) of infectious pancreatic necrosis (IPN) virus per cell, and the titer of cell-associated and released virus was determined from...
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ftpubmed:oai:pubmedcentral.nih.gov:422840 2023-05-15T15:30:27+02:00 Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures Piper, Donald Nicholson, Bruce L. Dunn, James 1973-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC422840 http://www.ncbi.nlm.nih.gov/pubmed/4199156 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC422840 http://www.ncbi.nlm.nih.gov/pubmed/4199156 Copyright © 1973 American Society for Microbiology Viral Infections Text 1973 ftpubmed 2013-08-29T23:47:44Z Cell cultures of trout gonad tissue (RTG-2) and Atlantic salmon heart, kidney, liver, and spleen tissue were inoculated with 50 50% tissue culture infective doses (TCID50) of infectious pancreatic necrosis (IPN) virus per cell, and the titer of cell-associated and released virus was determined from 2 to 16 h postinoculation (PI). Cover slips were collected over the same period and stained for IPN viral antigen by the direct immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 2 to 4 h and reached a peak titer of 108.2 to 108.4 TCID50 per ml at 8 to 10 h PI. The release of virus was more rapid in Atlantic salmon cells than in RTG-2 cells. Viral antigen was first detected by FA from 3 to 4 h PI. Approximately 75 to 80% of the cells contained antigen in the cytoplasm 9 to 11 h PI. The direct FA technique was found to be a sensitive method for detecting IPN virus in infected cells. Three strains of IPN virus were tested for serological cross reactions by FA and virus neutralization tests. Text Atlantic salmon PubMed Central (PMC) |
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English |
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Viral Infections |
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Viral Infections Piper, Donald Nicholson, Bruce L. Dunn, James Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures |
topic_facet |
Viral Infections |
description |
Cell cultures of trout gonad tissue (RTG-2) and Atlantic salmon heart, kidney, liver, and spleen tissue were inoculated with 50 50% tissue culture infective doses (TCID50) of infectious pancreatic necrosis (IPN) virus per cell, and the titer of cell-associated and released virus was determined from 2 to 16 h postinoculation (PI). Cover slips were collected over the same period and stained for IPN viral antigen by the direct immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 2 to 4 h and reached a peak titer of 108.2 to 108.4 TCID50 per ml at 8 to 10 h PI. The release of virus was more rapid in Atlantic salmon cells than in RTG-2 cells. Viral antigen was first detected by FA from 3 to 4 h PI. Approximately 75 to 80% of the cells contained antigen in the cytoplasm 9 to 11 h PI. The direct FA technique was found to be a sensitive method for detecting IPN virus in infected cells. Three strains of IPN virus were tested for serological cross reactions by FA and virus neutralization tests. |
format |
Text |
author |
Piper, Donald Nicholson, Bruce L. Dunn, James |
author_facet |
Piper, Donald Nicholson, Bruce L. Dunn, James |
author_sort |
Piper, Donald |
title |
Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures |
title_short |
Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures |
title_full |
Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures |
title_fullStr |
Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures |
title_full_unstemmed |
Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures |
title_sort |
immunofluorescent study of the replication of infectious pancreatic necrosis virus in trout and atlantic salmon cell cultures |
publishDate |
1973 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC422840 http://www.ncbi.nlm.nih.gov/pubmed/4199156 |
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Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC422840 http://www.ncbi.nlm.nih.gov/pubmed/4199156 |
op_rights |
Copyright © 1973 American Society for Microbiology |
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1766360906850107392 |