Picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities
Polar pelagic microbial communities access a narrower range of polysaccharide substrates than communities at lower latitudes. For example, the glucose-containing polysaccharide pullulan is typically not hydrolyzed in fjord waters of Svalbard, even though pullulan is rapidly hydrolyzed in sediments f...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , |
Format: | Text |
Language: | English |
Published: |
Frontiers Media S.A.
2014
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189390 http://www.ncbi.nlm.nih.gov/pubmed/25339946 https://doi.org/10.3389/fmicb.2014.00527 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:4189390 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:4189390 2023-05-15T16:55:57+02:00 Picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities Steen, Andrew D. Arnosti, Carol 2014-10-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189390 http://www.ncbi.nlm.nih.gov/pubmed/25339946 https://doi.org/10.3389/fmicb.2014.00527 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25339946 http://dx.doi.org/10.3389/fmicb.2014.00527 Copyright © 2014 Steen and Arnosti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2014 ftpubmed https://doi.org/10.3389/fmicb.2014.00527 2014-10-26T00:01:41Z Polar pelagic microbial communities access a narrower range of polysaccharide substrates than communities at lower latitudes. For example, the glucose-containing polysaccharide pullulan is typically not hydrolyzed in fjord waters of Svalbard, even though pullulan is rapidly hydrolyzed in sediments from Svalbard fjords, other polysaccharides are hydrolyzed rapidly in Svalbard waters, and pullulan is hydrolyzed rapidly in temperate waters. The purpose of this study was to investigate potential factors preventing hydrolysis of pullulan in Svalbard fjord waters. To this end, in two separate years, water from Isfjorden, Svalbard, was amended with different carbon sources and/or additional nutrients in order to determine whether increasing the concentration of these potentially-limiting factors would lead to measurable enzymatic activity. Addition of nitrate, phosphate, glucose, or amino acids did not yield detectable pullulan hydrolysis. The only treatment that led to detectable pullulan hydrolysis was extended incubation after the addition of maltotriose (a subunit of pullulan, and potential inducer of pullulanase). In these fjords, the ability to enzymatically access pullulan is likely confined to numerically minor members of the pelagic microbial community. These results are consistent with the hypothesis that pelagic microbial communities at high latitudes exhibit streamlined functionality, focused on a narrower range of substrates, than their temperate counterparts. Text Isfjord* Isfjorden Svalbard PubMed Central (PMC) Svalbard Frontiers in Microbiology 5 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbiology |
spellingShingle |
Microbiology Steen, Andrew D. Arnosti, Carol Picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities |
topic_facet |
Microbiology |
description |
Polar pelagic microbial communities access a narrower range of polysaccharide substrates than communities at lower latitudes. For example, the glucose-containing polysaccharide pullulan is typically not hydrolyzed in fjord waters of Svalbard, even though pullulan is rapidly hydrolyzed in sediments from Svalbard fjords, other polysaccharides are hydrolyzed rapidly in Svalbard waters, and pullulan is hydrolyzed rapidly in temperate waters. The purpose of this study was to investigate potential factors preventing hydrolysis of pullulan in Svalbard fjord waters. To this end, in two separate years, water from Isfjorden, Svalbard, was amended with different carbon sources and/or additional nutrients in order to determine whether increasing the concentration of these potentially-limiting factors would lead to measurable enzymatic activity. Addition of nitrate, phosphate, glucose, or amino acids did not yield detectable pullulan hydrolysis. The only treatment that led to detectable pullulan hydrolysis was extended incubation after the addition of maltotriose (a subunit of pullulan, and potential inducer of pullulanase). In these fjords, the ability to enzymatically access pullulan is likely confined to numerically minor members of the pelagic microbial community. These results are consistent with the hypothesis that pelagic microbial communities at high latitudes exhibit streamlined functionality, focused on a narrower range of substrates, than their temperate counterparts. |
format |
Text |
author |
Steen, Andrew D. Arnosti, Carol |
author_facet |
Steen, Andrew D. Arnosti, Carol |
author_sort |
Steen, Andrew D. |
title |
Picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities |
title_short |
Picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities |
title_full |
Picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities |
title_fullStr |
Picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities |
title_full_unstemmed |
Picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities |
title_sort |
picky, hungry eaters in the cold: persistent substrate selectivity among polar pelagic microbial communities |
publisher |
Frontiers Media S.A. |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189390 http://www.ncbi.nlm.nih.gov/pubmed/25339946 https://doi.org/10.3389/fmicb.2014.00527 |
geographic |
Svalbard |
geographic_facet |
Svalbard |
genre |
Isfjord* Isfjorden Svalbard |
genre_facet |
Isfjord* Isfjorden Svalbard |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25339946 http://dx.doi.org/10.3389/fmicb.2014.00527 |
op_rights |
Copyright © 2014 Steen and Arnosti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2014.00527 |
container_title |
Frontiers in Microbiology |
container_volume |
5 |
_version_ |
1766046984913813504 |