Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus
Antarctic soils represent a unique environment characterised by extremes of temperature, salinity, elevated UV radiation, low nutrient and low water content. Despite the harshness of this environment, members of 15 bacterial phyla have been identified in soils of the Ross Sea Region (RSR). However,...
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ftpubmed:oai:pubmedcentral.nih.gov:4186907 2023-05-15T13:50:36+02:00 Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus Dsouza, Melissa Taylor, Michael W. Turner, Susan J. Aislabie, Jackie 2014-10-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186907 http://www.ncbi.nlm.nih.gov/pubmed/25285990 https://doi.org/10.1371/journal.pone.0108009 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25285990 http://dx.doi.org/10.1371/journal.pone.0108009 This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2014 ftpubmed https://doi.org/10.1371/journal.pone.0108009 2014-10-19T00:57:03Z Antarctic soils represent a unique environment characterised by extremes of temperature, salinity, elevated UV radiation, low nutrient and low water content. Despite the harshness of this environment, members of 15 bacterial phyla have been identified in soils of the Ross Sea Region (RSR). However, the survival mechanisms and ecological roles of these phyla are largely unknown. The aim of this study was to investigate whether strains of Paenibacillus darwinianus owe their resilience to substantial genomic changes. For this, genome-based comparative analyses were performed on three P. darwinianus strains, isolated from gamma-irradiated RSR soils, together with nine temperate, soil-dwelling Paenibacillus spp. The genome of each strain was sequenced to over 1,000-fold coverage, then assembled into contigs totalling approximately 3 Mbp per genome. Based on the occurrence of essential, single-copy genes, genome completeness was estimated at approximately 88%. Genome analysis revealed between 3,043–3,091 protein-coding sequences (CDSs), primarily associated with two-component systems, sigma factors, transporters, sporulation and genes induced by cold-shock, oxidative and osmotic stresses. These comparative analyses provide an insight into the metabolic potential of P. darwinianus, revealing potential adaptive mechanisms for survival in Antarctic soils. However, a large proportion of these mechanisms were also identified in temperate Paenibacillus spp., suggesting that these mechanisms are beneficial for growth and survival in a range of soil environments. These analyses have also revealed that the P. darwinianus genomes contain significantly fewer CDSs and have a lower paralogous content. Notwithstanding the incompleteness of the assemblies, the large differences in genome sizes, determined by the number of genes in paralogous clusters and the CDS content, are indicative of genome content scaling. Finally, these sequences are a resource for further investigations into the expression of physiological attributes that ... Text Antarc* Antarctic Ross Sea PubMed Central (PMC) Antarctic Ross Sea PLoS ONE 9 10 e108009 |
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Research Article Dsouza, Melissa Taylor, Michael W. Turner, Susan J. Aislabie, Jackie Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus |
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Research Article |
description |
Antarctic soils represent a unique environment characterised by extremes of temperature, salinity, elevated UV radiation, low nutrient and low water content. Despite the harshness of this environment, members of 15 bacterial phyla have been identified in soils of the Ross Sea Region (RSR). However, the survival mechanisms and ecological roles of these phyla are largely unknown. The aim of this study was to investigate whether strains of Paenibacillus darwinianus owe their resilience to substantial genomic changes. For this, genome-based comparative analyses were performed on three P. darwinianus strains, isolated from gamma-irradiated RSR soils, together with nine temperate, soil-dwelling Paenibacillus spp. The genome of each strain was sequenced to over 1,000-fold coverage, then assembled into contigs totalling approximately 3 Mbp per genome. Based on the occurrence of essential, single-copy genes, genome completeness was estimated at approximately 88%. Genome analysis revealed between 3,043–3,091 protein-coding sequences (CDSs), primarily associated with two-component systems, sigma factors, transporters, sporulation and genes induced by cold-shock, oxidative and osmotic stresses. These comparative analyses provide an insight into the metabolic potential of P. darwinianus, revealing potential adaptive mechanisms for survival in Antarctic soils. However, a large proportion of these mechanisms were also identified in temperate Paenibacillus spp., suggesting that these mechanisms are beneficial for growth and survival in a range of soil environments. These analyses have also revealed that the P. darwinianus genomes contain significantly fewer CDSs and have a lower paralogous content. Notwithstanding the incompleteness of the assemblies, the large differences in genome sizes, determined by the number of genes in paralogous clusters and the CDS content, are indicative of genome content scaling. Finally, these sequences are a resource for further investigations into the expression of physiological attributes that ... |
format |
Text |
author |
Dsouza, Melissa Taylor, Michael W. Turner, Susan J. Aislabie, Jackie |
author_facet |
Dsouza, Melissa Taylor, Michael W. Turner, Susan J. Aislabie, Jackie |
author_sort |
Dsouza, Melissa |
title |
Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus |
title_short |
Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus |
title_full |
Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus |
title_fullStr |
Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus |
title_full_unstemmed |
Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus |
title_sort |
genome-based comparative analyses of antarctic and temperate species of paenibacillus |
publisher |
Public Library of Science |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186907 http://www.ncbi.nlm.nih.gov/pubmed/25285990 https://doi.org/10.1371/journal.pone.0108009 |
geographic |
Antarctic Ross Sea |
geographic_facet |
Antarctic Ross Sea |
genre |
Antarc* Antarctic Ross Sea |
genre_facet |
Antarc* Antarctic Ross Sea |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25285990 http://dx.doi.org/10.1371/journal.pone.0108009 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0108009 |
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PLoS ONE |
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9 |
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e108009 |
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