Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons
How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined 15N uptake rate measurements for ammonium, nitrate, and urea with 15N- and 13C-based DNA...
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ftpubmed:oai:pubmedcentral.nih.gov:4178671 2023-05-15T14:51:11+02:00 Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris 2014-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178671 http://www.ncbi.nlm.nih.gov/pubmed/25063662 https://doi.org/10.1128/AEM.01431-14 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25063662 http://dx.doi.org/10.1128/AEM.01431-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved. Environmental Microbiology Text 2014 ftpubmed https://doi.org/10.1128/AEM.01431-14 2015-04-05T00:05:14Z How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined 15N uptake rate measurements for ammonium, nitrate, and urea with 15N- and 13C-based DNA stable-isotope probing (SIP). The objective was to identify active bacterial and archeal plankton and their role in N and C uptake during the Arctic summer and winter seasons. We hypothesized that bacteria and archaea would successfully compete for nitrate and urea during the Arctic winter but not during the summer, when phytoplankton dominate the uptake of these nitrogen sources. Samples were collected at a coastal station near Barrow, AK, during August and January. During both seasons, ammonium uptake rates were greater than those for nitrate or urea, and nitrate uptake rates remained lower than those for ammonium or urea. SIP experiments indicated a strong seasonal shift of bacterial and archaeal N utilization from ammonium during the summer to urea during the winter but did not support a similar seasonal pattern of nitrate utilization. Analysis of 16S rRNA gene sequences obtained from each SIP fraction implicated marine group I Crenarchaeota (MGIC) as well as Betaproteobacteria, Firmicutes, SAR11, and SAR324 in N uptake from urea during the winter. Similarly, 13C SIP data suggested dark carbon fixation for MGIC, as well as for several proteobacterial lineages and the Firmicutes. These data are consistent with urea-fueled nitrification by polar archaea and bacteria, which may be advantageous under dark conditions. Text Arctic Climate change Phytoplankton PubMed Central (PMC) Arctic Applied and Environmental Microbiology 80 19 6013 6022 |
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English |
topic |
Environmental Microbiology |
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Environmental Microbiology Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
topic_facet |
Environmental Microbiology |
description |
How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined 15N uptake rate measurements for ammonium, nitrate, and urea with 15N- and 13C-based DNA stable-isotope probing (SIP). The objective was to identify active bacterial and archeal plankton and their role in N and C uptake during the Arctic summer and winter seasons. We hypothesized that bacteria and archaea would successfully compete for nitrate and urea during the Arctic winter but not during the summer, when phytoplankton dominate the uptake of these nitrogen sources. Samples were collected at a coastal station near Barrow, AK, during August and January. During both seasons, ammonium uptake rates were greater than those for nitrate or urea, and nitrate uptake rates remained lower than those for ammonium or urea. SIP experiments indicated a strong seasonal shift of bacterial and archaeal N utilization from ammonium during the summer to urea during the winter but did not support a similar seasonal pattern of nitrate utilization. Analysis of 16S rRNA gene sequences obtained from each SIP fraction implicated marine group I Crenarchaeota (MGIC) as well as Betaproteobacteria, Firmicutes, SAR11, and SAR324 in N uptake from urea during the winter. Similarly, 13C SIP data suggested dark carbon fixation for MGIC, as well as for several proteobacterial lineages and the Firmicutes. These data are consistent with urea-fueled nitrification by polar archaea and bacteria, which may be advantageous under dark conditions. |
format |
Text |
author |
Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris |
author_facet |
Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris |
author_sort |
Connelly, Tara L. |
title |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_short |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_full |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_fullStr |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_full_unstemmed |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_sort |
urea uptake and carbon fixation by marine pelagic bacteria and archaea during the arctic summer and winter seasons |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178671 http://www.ncbi.nlm.nih.gov/pubmed/25063662 https://doi.org/10.1128/AEM.01431-14 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change Phytoplankton |
genre_facet |
Arctic Climate change Phytoplankton |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25063662 http://dx.doi.org/10.1128/AEM.01431-14 |
op_rights |
Copyright © 2014, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.01431-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
19 |
container_start_page |
6013 |
op_container_end_page |
6022 |
_version_ |
1766322236897099776 |