Effects of Season and Host Physiological State on the Diversity, Density, and Activity of the Arctic Ground Squirrel Cecal Microbiota
We examined the seasonal changes of the cecal microbiota of captive arctic ground squirrels (Urocitellus parryii) by measuring microbial diversity and composition, total bacterial density and viability, and short-chain fatty acid concentrations at four sample periods (summer, torpor, interbout arous...
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American Society for Microbiology
2014
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178600 http://www.ncbi.nlm.nih.gov/pubmed/25002417 https://doi.org/10.1128/AEM.01537-14 |
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ftpubmed:oai:pubmedcentral.nih.gov:4178600 2023-05-15T14:31:28+02:00 Effects of Season and Host Physiological State on the Diversity, Density, and Activity of the Arctic Ground Squirrel Cecal Microbiota Stevenson, Timothy J. Duddleston, Khrystyne N. Buck, C. Loren 2014-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178600 http://www.ncbi.nlm.nih.gov/pubmed/25002417 https://doi.org/10.1128/AEM.01537-14 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25002417 http://dx.doi.org/10.1128/AEM.01537-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved. Microbial Ecology Text 2014 ftpubmed https://doi.org/10.1128/AEM.01537-14 2015-03-08T01:11:22Z We examined the seasonal changes of the cecal microbiota of captive arctic ground squirrels (Urocitellus parryii) by measuring microbial diversity and composition, total bacterial density and viability, and short-chain fatty acid concentrations at four sample periods (summer, torpor, interbout arousal, and posthibernation). Abundance of Firmicutes was lower, whereas abundances of Bacteroidetes, Verrucomicrobia, and Proteobacteria were higher during torpor and interbout arousal than in summer. Bacterial densities and percentages of live bacteria were significantly higher in summer than during torpor and interbout arousal. Likewise, total short-chain fatty acid concentrations were significantly greater during summer than during torpor and interbout arousal. Concentrations of individual short-chain fatty acids varied across sample periods, with butyrate concentrations higher and acetate concentrations lower during summer than at all other sample periods. Characteristics of the gut community posthibernation were more similar to those during torpor and interbout arousal than to those during summer. However, higher abundances of the genera Bacteroides and Akkermansia occurred during posthibernation than during interbout arousal and torpor. Collectively, our results clearly demonstrate that seasonal changes in physiology associated with hibernation and activity affect the gut microbial community in the arctic ground squirrel. Importantly, similarities between the gut microbiota of arctic ground squirrels and thirteen-lined ground squirrels suggest the potential for a core microbiota during hibernation. Text Arctic ground squirrel Arctic Urocitellus parryii PubMed Central (PMC) Arctic Applied and Environmental Microbiology 80 18 5611 5622 |
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ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Stevenson, Timothy J. Duddleston, Khrystyne N. Buck, C. Loren Effects of Season and Host Physiological State on the Diversity, Density, and Activity of the Arctic Ground Squirrel Cecal Microbiota |
topic_facet |
Microbial Ecology |
description |
We examined the seasonal changes of the cecal microbiota of captive arctic ground squirrels (Urocitellus parryii) by measuring microbial diversity and composition, total bacterial density and viability, and short-chain fatty acid concentrations at four sample periods (summer, torpor, interbout arousal, and posthibernation). Abundance of Firmicutes was lower, whereas abundances of Bacteroidetes, Verrucomicrobia, and Proteobacteria were higher during torpor and interbout arousal than in summer. Bacterial densities and percentages of live bacteria were significantly higher in summer than during torpor and interbout arousal. Likewise, total short-chain fatty acid concentrations were significantly greater during summer than during torpor and interbout arousal. Concentrations of individual short-chain fatty acids varied across sample periods, with butyrate concentrations higher and acetate concentrations lower during summer than at all other sample periods. Characteristics of the gut community posthibernation were more similar to those during torpor and interbout arousal than to those during summer. However, higher abundances of the genera Bacteroides and Akkermansia occurred during posthibernation than during interbout arousal and torpor. Collectively, our results clearly demonstrate that seasonal changes in physiology associated with hibernation and activity affect the gut microbial community in the arctic ground squirrel. Importantly, similarities between the gut microbiota of arctic ground squirrels and thirteen-lined ground squirrels suggest the potential for a core microbiota during hibernation. |
format |
Text |
author |
Stevenson, Timothy J. Duddleston, Khrystyne N. Buck, C. Loren |
author_facet |
Stevenson, Timothy J. Duddleston, Khrystyne N. Buck, C. Loren |
author_sort |
Stevenson, Timothy J. |
title |
Effects of Season and Host Physiological State on the Diversity, Density, and Activity of the Arctic Ground Squirrel Cecal Microbiota |
title_short |
Effects of Season and Host Physiological State on the Diversity, Density, and Activity of the Arctic Ground Squirrel Cecal Microbiota |
title_full |
Effects of Season and Host Physiological State on the Diversity, Density, and Activity of the Arctic Ground Squirrel Cecal Microbiota |
title_fullStr |
Effects of Season and Host Physiological State on the Diversity, Density, and Activity of the Arctic Ground Squirrel Cecal Microbiota |
title_full_unstemmed |
Effects of Season and Host Physiological State on the Diversity, Density, and Activity of the Arctic Ground Squirrel Cecal Microbiota |
title_sort |
effects of season and host physiological state on the diversity, density, and activity of the arctic ground squirrel cecal microbiota |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178600 http://www.ncbi.nlm.nih.gov/pubmed/25002417 https://doi.org/10.1128/AEM.01537-14 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic ground squirrel Arctic Urocitellus parryii |
genre_facet |
Arctic ground squirrel Arctic Urocitellus parryii |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25002417 http://dx.doi.org/10.1128/AEM.01537-14 |
op_rights |
Copyright © 2014, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.01537-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
18 |
container_start_page |
5611 |
op_container_end_page |
5622 |
_version_ |
1766305091814424576 |