Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia)
We report on a novel arctic strain BM1 of a carotenogenic chlorophyte from a coastal habitat with harsh environmental conditions (wide variations in solar irradiance, temperature, salinity and nutrient availability) identified as Haematococcus pluvialis Flotow. Increased (25‰) salinity exerted no ad...
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ftpubmed:oai:pubmedcentral.nih.gov:4145328 2023-05-15T15:07:06+02:00 Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) Chekanov, Konstantin Lobakova, Elena Selyakh, Irina Semenova, Larisa Sidorov, Roman Solovchenko, Alexei 2014-08-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145328 https://doi.org/10.3390/md12084504 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://dx.doi.org/10.3390/md12084504 © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). CC-BY Article Text 2014 ftpubmed https://doi.org/10.3390/md12084504 2014-08-31T00:59:16Z We report on a novel arctic strain BM1 of a carotenogenic chlorophyte from a coastal habitat with harsh environmental conditions (wide variations in solar irradiance, temperature, salinity and nutrient availability) identified as Haematococcus pluvialis Flotow. Increased (25‰) salinity exerted no adverse effect on the growth of the green BM1 cells. Under stressful conditions (high light, nitrogen and phosphorus deprivation), green vegetative cells of H. pluvialis BM1 grown in BG11 medium formed non-motile palmelloid cells and, eventually, hematocysts capable of a massive accumulation of the keto-carotenoid astaxanthin with a high nutraceutical and therapeutic potential. Routinely, astaxanthin was accumulated at the level of 4% of the cell dry weight (DW), reaching, under prolonged stress, 5.5% DW. Astaxanthin was predominantly accumulated in the form of mono- and diesters of fatty acids from C16 and C18 families. The palmelloids and hematocysts were characterized by the formation of red-colored cytoplasmic lipid droplets, increasingly large in size and number. The lipid droplets tended to merge and occupied almost the entire volume of the cell at the advanced stages of stress-induced carotenogenesis. The potential application of the new strain for the production of astaxanthin is discussed in comparison with the H. pluvialis strains currently employed in microalgal biotechnology. Text Arctic White Sea PubMed Central (PMC) Arctic White Sea Marine Drugs 12 8 4504 4520 |
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Article Chekanov, Konstantin Lobakova, Elena Selyakh, Irina Semenova, Larisa Sidorov, Roman Solovchenko, Alexei Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
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We report on a novel arctic strain BM1 of a carotenogenic chlorophyte from a coastal habitat with harsh environmental conditions (wide variations in solar irradiance, temperature, salinity and nutrient availability) identified as Haematococcus pluvialis Flotow. Increased (25‰) salinity exerted no adverse effect on the growth of the green BM1 cells. Under stressful conditions (high light, nitrogen and phosphorus deprivation), green vegetative cells of H. pluvialis BM1 grown in BG11 medium formed non-motile palmelloid cells and, eventually, hematocysts capable of a massive accumulation of the keto-carotenoid astaxanthin with a high nutraceutical and therapeutic potential. Routinely, astaxanthin was accumulated at the level of 4% of the cell dry weight (DW), reaching, under prolonged stress, 5.5% DW. Astaxanthin was predominantly accumulated in the form of mono- and diesters of fatty acids from C16 and C18 families. The palmelloids and hematocysts were characterized by the formation of red-colored cytoplasmic lipid droplets, increasingly large in size and number. The lipid droplets tended to merge and occupied almost the entire volume of the cell at the advanced stages of stress-induced carotenogenesis. The potential application of the new strain for the production of astaxanthin is discussed in comparison with the H. pluvialis strains currently employed in microalgal biotechnology. |
format |
Text |
author |
Chekanov, Konstantin Lobakova, Elena Selyakh, Irina Semenova, Larisa Sidorov, Roman Solovchenko, Alexei |
author_facet |
Chekanov, Konstantin Lobakova, Elena Selyakh, Irina Semenova, Larisa Sidorov, Roman Solovchenko, Alexei |
author_sort |
Chekanov, Konstantin |
title |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_short |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_full |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_fullStr |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_full_unstemmed |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_sort |
accumulation of astaxanthin by a new haematococcus pluvialis strain bm1 from the white sea coastal rocks (russia) |
publisher |
MDPI |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145328 https://doi.org/10.3390/md12084504 |
geographic |
Arctic White Sea |
geographic_facet |
Arctic White Sea |
genre |
Arctic White Sea |
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Arctic White Sea |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://dx.doi.org/10.3390/md12084504 |
op_rights |
© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md12084504 |
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Marine Drugs |
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12 |
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4520 |
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