Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus
Multiple drug resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as a community acquired infection. As a result limited treatment options are available with conventional synthetic antibiotics. Bioprospecting natural products with potent antimicr...
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ftpubmed:oai:pubmedcentral.nih.gov:4131597 2023-05-15T13:31:59+02:00 Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus Huang, Jonathan P. Mojib, Nazia Goli, Rakesh R. Watkins, Samantha Waites, Ken B. Ravindra, Rasik Andersen, Dale T. Bej, Asim K. 2012-04-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131597 https://doi.org/10.1007/s13659-012-0021-4 en eng Springer-Verlag http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://dx.doi.org/10.1007/s13659-012-0021-4 © The Author(s) 2012 Regular Article Text 2012 ftpubmed https://doi.org/10.1007/s13659-012-0021-4 2014-08-24T00:49:04Z Multiple drug resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as a community acquired infection. As a result limited treatment options are available with conventional synthetic antibiotics. Bioprospecting natural products with potent antimicrobial activity show promise for developing new drugs against this pathogen. In this study, we have investigated the antimicrobial activity of a purple violet pigment (PVP) from an Antarctic bacterium, Janthinobacterium sp. Ant5-2 on 15 clinical MDR and MRSA strains. The colorimetric resazurin assay was employed to determine the minimum inhibitory concentration (MIC90) of PVP against MDR and MRSA. The MIC90 ranged between 1.57 µg/mL and 3.13 µg/mL, which are significantly lower than many antimicrobials tested from natural sources against this pathogen. The spectrophotometrically determined growth analysis and total microscopic counts using Live/dead® BacLight™ fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC levels. In silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial compound. The continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens. Text Antarc* Antarctic PubMed Central (PMC) Antarctic Natural Products and Bioprospecting 2 3 104 110 |
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Regular Article |
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Regular Article Huang, Jonathan P. Mojib, Nazia Goli, Rakesh R. Watkins, Samantha Waites, Ken B. Ravindra, Rasik Andersen, Dale T. Bej, Asim K. Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus |
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Regular Article |
description |
Multiple drug resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as a community acquired infection. As a result limited treatment options are available with conventional synthetic antibiotics. Bioprospecting natural products with potent antimicrobial activity show promise for developing new drugs against this pathogen. In this study, we have investigated the antimicrobial activity of a purple violet pigment (PVP) from an Antarctic bacterium, Janthinobacterium sp. Ant5-2 on 15 clinical MDR and MRSA strains. The colorimetric resazurin assay was employed to determine the minimum inhibitory concentration (MIC90) of PVP against MDR and MRSA. The MIC90 ranged between 1.57 µg/mL and 3.13 µg/mL, which are significantly lower than many antimicrobials tested from natural sources against this pathogen. The spectrophotometrically determined growth analysis and total microscopic counts using Live/dead® BacLight™ fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC levels. In silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial compound. The continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens. |
format |
Text |
author |
Huang, Jonathan P. Mojib, Nazia Goli, Rakesh R. Watkins, Samantha Waites, Ken B. Ravindra, Rasik Andersen, Dale T. Bej, Asim K. |
author_facet |
Huang, Jonathan P. Mojib, Nazia Goli, Rakesh R. Watkins, Samantha Waites, Ken B. Ravindra, Rasik Andersen, Dale T. Bej, Asim K. |
author_sort |
Huang, Jonathan P. |
title |
Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus |
title_short |
Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus |
title_full |
Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus |
title_fullStr |
Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus |
title_full_unstemmed |
Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus |
title_sort |
antimicrobial activity of pvp from an antarctic bacterium, janthinobacterium sp. ant5-2, on multi-drug and methicillin resistant staphylococcus aureus |
publisher |
Springer-Verlag |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131597 https://doi.org/10.1007/s13659-012-0021-4 |
geographic |
Antarctic |
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Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://dx.doi.org/10.1007/s13659-012-0021-4 |
op_rights |
© The Author(s) 2012 |
op_doi |
https://doi.org/10.1007/s13659-012-0021-4 |
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Natural Products and Bioprospecting |
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2 |
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3 |
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104 |
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110 |
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1766023410145558528 |