Snow Surface Microbiome on the High Antarctic Plateau (DOME C)
The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In...
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ftpubmed:oai:pubmedcentral.nih.gov:4125213 2023-05-15T13:58:32+02:00 Snow Surface Microbiome on the High Antarctic Plateau (DOME C) Michaud, Luigi Lo Giudice, Angelina Mysara, Mohamed Monsieurs, Pieter Raffa, Carmela Leys, Natalie Amalfitano, Stefano Van Houdt, Rob 2014-08-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125213 http://www.ncbi.nlm.nih.gov/pubmed/25101779 https://doi.org/10.1371/journal.pone.0104505 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25101779 http://dx.doi.org/10.1371/journal.pone.0104505 This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2014 ftpubmed https://doi.org/10.1371/journal.pone.0104505 2014-08-17T00:57:51Z The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In this study, we investigated the microbial diversity in the surface snow surrounding the Concordia Research Station on the High Antarctic Plateau through a polyphasic approach, including direct prokaryotic quantification by flow cytometry and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), and phylogenetic identification by 16S RNA gene clone library sequencing and 454 16S amplicon pyrosequencing. Although the microbial abundance was low (<103 cells/ml of snowmelt), concordant results were obtained with the different techniques. The microbial community was mainly composed of members of the Alpha-proteobacteria class (e.g. Kiloniellaceae and Rhodobacteraceae), which is one of the most well-represented bacterial groups in marine habitats, Bacteroidetes (e.g. Cryomorphaceae and Flavobacteriaceae) and Cyanobacteria. Based on our results, polar microorganisms could not only be considered as deposited airborne particles, but as an active component of the snowpack ecology of the High Antarctic Plateau. Text Antarc* Antarctic PubMed Central (PMC) Antarctic PLoS ONE 9 8 e104505 |
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Research Article Michaud, Luigi Lo Giudice, Angelina Mysara, Mohamed Monsieurs, Pieter Raffa, Carmela Leys, Natalie Amalfitano, Stefano Van Houdt, Rob Snow Surface Microbiome on the High Antarctic Plateau (DOME C) |
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Research Article |
description |
The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In this study, we investigated the microbial diversity in the surface snow surrounding the Concordia Research Station on the High Antarctic Plateau through a polyphasic approach, including direct prokaryotic quantification by flow cytometry and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), and phylogenetic identification by 16S RNA gene clone library sequencing and 454 16S amplicon pyrosequencing. Although the microbial abundance was low (<103 cells/ml of snowmelt), concordant results were obtained with the different techniques. The microbial community was mainly composed of members of the Alpha-proteobacteria class (e.g. Kiloniellaceae and Rhodobacteraceae), which is one of the most well-represented bacterial groups in marine habitats, Bacteroidetes (e.g. Cryomorphaceae and Flavobacteriaceae) and Cyanobacteria. Based on our results, polar microorganisms could not only be considered as deposited airborne particles, but as an active component of the snowpack ecology of the High Antarctic Plateau. |
format |
Text |
author |
Michaud, Luigi Lo Giudice, Angelina Mysara, Mohamed Monsieurs, Pieter Raffa, Carmela Leys, Natalie Amalfitano, Stefano Van Houdt, Rob |
author_facet |
Michaud, Luigi Lo Giudice, Angelina Mysara, Mohamed Monsieurs, Pieter Raffa, Carmela Leys, Natalie Amalfitano, Stefano Van Houdt, Rob |
author_sort |
Michaud, Luigi |
title |
Snow Surface Microbiome on the High Antarctic Plateau (DOME C) |
title_short |
Snow Surface Microbiome on the High Antarctic Plateau (DOME C) |
title_full |
Snow Surface Microbiome on the High Antarctic Plateau (DOME C) |
title_fullStr |
Snow Surface Microbiome on the High Antarctic Plateau (DOME C) |
title_full_unstemmed |
Snow Surface Microbiome on the High Antarctic Plateau (DOME C) |
title_sort |
snow surface microbiome on the high antarctic plateau (dome c) |
publisher |
Public Library of Science |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125213 http://www.ncbi.nlm.nih.gov/pubmed/25101779 https://doi.org/10.1371/journal.pone.0104505 |
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Antarctic |
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Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/25101779 http://dx.doi.org/10.1371/journal.pone.0104505 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
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https://doi.org/10.1371/journal.pone.0104505 |
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e104505 |
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