Resuscitation Promoting Factor (Rpf) from Tomitella biformata AHU 1821T Promotes Growth and Resuscitates Non-Dividing Cells
Functional variation of Rpf, a growth factor found exclusively in Actinobacteria, is differentiated by its source and amino acid sequences. Only purified Rpf proteins from three species have been studied so far. To seek new Rpfs for use in future studies to understand their role in Actinobacteria, t...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070687 http://www.ncbi.nlm.nih.gov/pubmed/23100022 https://doi.org/10.1264/jsme2.ME12122 |
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ftpubmed:oai:pubmedcentral.nih.gov:4070687 2023-05-15T16:37:52+02:00 Resuscitation Promoting Factor (Rpf) from Tomitella biformata AHU 1821T Promotes Growth and Resuscitates Non-Dividing Cells Puspita, Indun Dewi Uehara, Moe Katayama, Taiki Kikuchi, Yoshitomo Kitagawa, Wataru Kamagata, Yoichi Asano, Kozo Nakatsu, Cindy H. Tanaka, Michiko 2013-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070687 http://www.ncbi.nlm.nih.gov/pubmed/23100022 https://doi.org/10.1264/jsme2.ME12122 en eng Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23100022 http://dx.doi.org/10.1264/jsme2.ME12122 Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology Articles Text 2013 ftpubmed https://doi.org/10.1264/jsme2.ME12122 2014-07-27T00:37:21Z Functional variation of Rpf, a growth factor found exclusively in Actinobacteria, is differentiated by its source and amino acid sequences. Only purified Rpf proteins from three species have been studied so far. To seek new Rpfs for use in future studies to understand their role in Actinobacteria, the objective of this study was to identify rpf gene homologs in Tomitella biformata AHU 1821T, a novel Actinobacteria isolated from permafrost ice wedge. Amplification using degenerate primers targeting the essential Rpf domain led to the discovery of a new rpf gene in T. biformata. Gene structure and the deduced Rpf domain amino acid sequence indicated that this rpf gene was not identical to previously studied Rpf. Phylogenetic analysis placed T. biformata Rpf in a monophyletic branch in the RpfB subfamily. The deduced amino acid sequence was 44.9% identical to RpfB in Mycobacterium tuberculosis, the closest functionally tested Rpf. The gene was cloned and expressed in Escherichia coli; the recombinant Rpf protein (rRpf) promoted the growth of dividing cells and resuscitated non-dividing cells of T. biformata. Compared to other studies, this Rpf was required at higher concentrations to promote its growth and to resuscitate itself from a non-dividing state. The resuscitation function was likely due to the highly conserved Rpf domain. This study provides evidence that a genetically unique but functional Rpf can be found in novel members of Actinobacteria and can lead to a better understanding of bacterial cytokines in this phylum. Text Ice permafrost wedge* PubMed Central (PMC) Microbes and Environments 28 1 58 64 |
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Articles Puspita, Indun Dewi Uehara, Moe Katayama, Taiki Kikuchi, Yoshitomo Kitagawa, Wataru Kamagata, Yoichi Asano, Kozo Nakatsu, Cindy H. Tanaka, Michiko Resuscitation Promoting Factor (Rpf) from Tomitella biformata AHU 1821T Promotes Growth and Resuscitates Non-Dividing Cells |
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Functional variation of Rpf, a growth factor found exclusively in Actinobacteria, is differentiated by its source and amino acid sequences. Only purified Rpf proteins from three species have been studied so far. To seek new Rpfs for use in future studies to understand their role in Actinobacteria, the objective of this study was to identify rpf gene homologs in Tomitella biformata AHU 1821T, a novel Actinobacteria isolated from permafrost ice wedge. Amplification using degenerate primers targeting the essential Rpf domain led to the discovery of a new rpf gene in T. biformata. Gene structure and the deduced Rpf domain amino acid sequence indicated that this rpf gene was not identical to previously studied Rpf. Phylogenetic analysis placed T. biformata Rpf in a monophyletic branch in the RpfB subfamily. The deduced amino acid sequence was 44.9% identical to RpfB in Mycobacterium tuberculosis, the closest functionally tested Rpf. The gene was cloned and expressed in Escherichia coli; the recombinant Rpf protein (rRpf) promoted the growth of dividing cells and resuscitated non-dividing cells of T. biformata. Compared to other studies, this Rpf was required at higher concentrations to promote its growth and to resuscitate itself from a non-dividing state. The resuscitation function was likely due to the highly conserved Rpf domain. This study provides evidence that a genetically unique but functional Rpf can be found in novel members of Actinobacteria and can lead to a better understanding of bacterial cytokines in this phylum. |
format |
Text |
author |
Puspita, Indun Dewi Uehara, Moe Katayama, Taiki Kikuchi, Yoshitomo Kitagawa, Wataru Kamagata, Yoichi Asano, Kozo Nakatsu, Cindy H. Tanaka, Michiko |
author_facet |
Puspita, Indun Dewi Uehara, Moe Katayama, Taiki Kikuchi, Yoshitomo Kitagawa, Wataru Kamagata, Yoichi Asano, Kozo Nakatsu, Cindy H. Tanaka, Michiko |
author_sort |
Puspita, Indun Dewi |
title |
Resuscitation Promoting Factor (Rpf) from Tomitella biformata AHU 1821T Promotes Growth and Resuscitates Non-Dividing Cells |
title_short |
Resuscitation Promoting Factor (Rpf) from Tomitella biformata AHU 1821T Promotes Growth and Resuscitates Non-Dividing Cells |
title_full |
Resuscitation Promoting Factor (Rpf) from Tomitella biformata AHU 1821T Promotes Growth and Resuscitates Non-Dividing Cells |
title_fullStr |
Resuscitation Promoting Factor (Rpf) from Tomitella biformata AHU 1821T Promotes Growth and Resuscitates Non-Dividing Cells |
title_full_unstemmed |
Resuscitation Promoting Factor (Rpf) from Tomitella biformata AHU 1821T Promotes Growth and Resuscitates Non-Dividing Cells |
title_sort |
resuscitation promoting factor (rpf) from tomitella biformata ahu 1821t promotes growth and resuscitates non-dividing cells |
publisher |
Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070687 http://www.ncbi.nlm.nih.gov/pubmed/23100022 https://doi.org/10.1264/jsme2.ME12122 |
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Ice permafrost wedge* |
genre_facet |
Ice permafrost wedge* |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23100022 http://dx.doi.org/10.1264/jsme2.ME12122 |
op_rights |
Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology |
op_doi |
https://doi.org/10.1264/jsme2.ME12122 |
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Microbes and Environments |
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28 |
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1 |
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58 |
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64 |
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1766028158789746688 |