Bt-maize (MON810) and Non-GM Soybean Meal in Diets for Atlantic Salmon (Salmo salar L.) Juveniles – Impact on Survival, Growth Performance, Development, Digestive Function, and Transcriptional Expression of Intestinal Immune and Stress Responses
Responses in Atlantic salmon (Salmo salar L.) juveniles (fry) fed diets containing genetically modified maize (Bt-maize, MON810) expressing Cry1Ab protein from first-feeding were investigated during a 99-day feeding trial. Four experimental diets were made; each diet contained ∼20% maize, either Bt-...
Published in: | PLoS ONE |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
Public Library of Science
2014
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055707 http://www.ncbi.nlm.nih.gov/pubmed/24923786 https://doi.org/10.1371/journal.pone.0099932 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:4055707 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:4055707 2023-05-15T15:32:04+02:00 Bt-maize (MON810) and Non-GM Soybean Meal in Diets for Atlantic Salmon (Salmo salar L.) Juveniles – Impact on Survival, Growth Performance, Development, Digestive Function, and Transcriptional Expression of Intestinal Immune and Stress Responses Gu, Jinni Bakke, Anne Marie Valen, Elin C. Lein, Ingrid Krogdahl, Åshild 2014-06-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055707 http://www.ncbi.nlm.nih.gov/pubmed/24923786 https://doi.org/10.1371/journal.pone.0099932 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24923786 http://dx.doi.org/10.1371/journal.pone.0099932 This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2014 ftpubmed https://doi.org/10.1371/journal.pone.0099932 2014-06-22T00:57:09Z Responses in Atlantic salmon (Salmo salar L.) juveniles (fry) fed diets containing genetically modified maize (Bt-maize, MON810) expressing Cry1Ab protein from first-feeding were investigated during a 99-day feeding trial. Four experimental diets were made; each diet contained ∼20% maize, either Bt-maize or its near-isogenic maternal line (non-GM maize). One pair was fishmeal-based while the other pair included standard (extracted) soybean meal (SBM; 16.7% inclusion level), with the intention of investigating responses to the maize varieties in healthy fish as well as in immunologically challenged fish with SBM-induced distal intestinal inflammation, respectively. Three replicate tanks of fry (0.17±0.01 g; initial mean weight ± SEM) were fed one of the four diets and samples were taken on days 15, 36, 48 and 99. Survival, growth performance, whole body composition, digestive function, morphology of intestine, liver and skeleton, and mRNA expression of some immune and stress response parameters in the distal intestine were evaluated. After 99 days of feeding, survival was enhanced and the intended SBM-induced inflammatory response in the distal intestine of the two groups of SBM-fed fish was absent, indicating that the juvenile salmon were tolerant to SBM. Mortality, growth performance and body composition were similar in fish fed the two maize varieties. The Bt-maize fed fish, however, displayed minor but significantly decreased digestive enzyme activities of leucine aminopeptidase and maltase, as well as decreased concentration of gut bile salts, but significantly increased amylase activity at some sampling points. Histomorphological, radiographic and mRNA expression evaluations did not reveal any biologically relevant effects of Bt-maize in the gastrointestinal tract, liver or skeleton. The results suggest that the Cry1Ab protein or other compositional differences in GM Bt-maize may cause minor alterations in intestinal responses in juvenile salmon, but without affecting overall survival, growth performance, ... Text Atlantic salmon Salmo salar PubMed Central (PMC) PLoS ONE 9 6 e99932 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Gu, Jinni Bakke, Anne Marie Valen, Elin C. Lein, Ingrid Krogdahl, Åshild Bt-maize (MON810) and Non-GM Soybean Meal in Diets for Atlantic Salmon (Salmo salar L.) Juveniles – Impact on Survival, Growth Performance, Development, Digestive Function, and Transcriptional Expression of Intestinal Immune and Stress Responses |
topic_facet |
Research Article |
description |
Responses in Atlantic salmon (Salmo salar L.) juveniles (fry) fed diets containing genetically modified maize (Bt-maize, MON810) expressing Cry1Ab protein from first-feeding were investigated during a 99-day feeding trial. Four experimental diets were made; each diet contained ∼20% maize, either Bt-maize or its near-isogenic maternal line (non-GM maize). One pair was fishmeal-based while the other pair included standard (extracted) soybean meal (SBM; 16.7% inclusion level), with the intention of investigating responses to the maize varieties in healthy fish as well as in immunologically challenged fish with SBM-induced distal intestinal inflammation, respectively. Three replicate tanks of fry (0.17±0.01 g; initial mean weight ± SEM) were fed one of the four diets and samples were taken on days 15, 36, 48 and 99. Survival, growth performance, whole body composition, digestive function, morphology of intestine, liver and skeleton, and mRNA expression of some immune and stress response parameters in the distal intestine were evaluated. After 99 days of feeding, survival was enhanced and the intended SBM-induced inflammatory response in the distal intestine of the two groups of SBM-fed fish was absent, indicating that the juvenile salmon were tolerant to SBM. Mortality, growth performance and body composition were similar in fish fed the two maize varieties. The Bt-maize fed fish, however, displayed minor but significantly decreased digestive enzyme activities of leucine aminopeptidase and maltase, as well as decreased concentration of gut bile salts, but significantly increased amylase activity at some sampling points. Histomorphological, radiographic and mRNA expression evaluations did not reveal any biologically relevant effects of Bt-maize in the gastrointestinal tract, liver or skeleton. The results suggest that the Cry1Ab protein or other compositional differences in GM Bt-maize may cause minor alterations in intestinal responses in juvenile salmon, but without affecting overall survival, growth performance, ... |
format |
Text |
author |
Gu, Jinni Bakke, Anne Marie Valen, Elin C. Lein, Ingrid Krogdahl, Åshild |
author_facet |
Gu, Jinni Bakke, Anne Marie Valen, Elin C. Lein, Ingrid Krogdahl, Åshild |
author_sort |
Gu, Jinni |
title |
Bt-maize (MON810) and Non-GM Soybean Meal in Diets for Atlantic Salmon (Salmo salar L.) Juveniles – Impact on Survival, Growth Performance, Development, Digestive Function, and Transcriptional Expression of Intestinal Immune and Stress Responses |
title_short |
Bt-maize (MON810) and Non-GM Soybean Meal in Diets for Atlantic Salmon (Salmo salar L.) Juveniles – Impact on Survival, Growth Performance, Development, Digestive Function, and Transcriptional Expression of Intestinal Immune and Stress Responses |
title_full |
Bt-maize (MON810) and Non-GM Soybean Meal in Diets for Atlantic Salmon (Salmo salar L.) Juveniles – Impact on Survival, Growth Performance, Development, Digestive Function, and Transcriptional Expression of Intestinal Immune and Stress Responses |
title_fullStr |
Bt-maize (MON810) and Non-GM Soybean Meal in Diets for Atlantic Salmon (Salmo salar L.) Juveniles – Impact on Survival, Growth Performance, Development, Digestive Function, and Transcriptional Expression of Intestinal Immune and Stress Responses |
title_full_unstemmed |
Bt-maize (MON810) and Non-GM Soybean Meal in Diets for Atlantic Salmon (Salmo salar L.) Juveniles – Impact on Survival, Growth Performance, Development, Digestive Function, and Transcriptional Expression of Intestinal Immune and Stress Responses |
title_sort |
bt-maize (mon810) and non-gm soybean meal in diets for atlantic salmon (salmo salar l.) juveniles – impact on survival, growth performance, development, digestive function, and transcriptional expression of intestinal immune and stress responses |
publisher |
Public Library of Science |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055707 http://www.ncbi.nlm.nih.gov/pubmed/24923786 https://doi.org/10.1371/journal.pone.0099932 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24923786 http://dx.doi.org/10.1371/journal.pone.0099932 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0099932 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
6 |
container_start_page |
e99932 |
_version_ |
1766362563288760320 |