Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces)
The occurrence of Anaplasma phagocytophilum was investigated in spleen and serum samples from Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples were analysed for presence of A. phagocytophilum DNA by real-time PCR (n = 263), and for Anaplasma antibodies with ELISA serolog...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045167 http://www.ncbi.nlm.nih.gov/pubmed/24001524 https://doi.org/10.1017/S0950268813002094 |
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ftpubmed:oai:pubmedcentral.nih.gov:4045167 2023-05-15T13:12:48+02:00 Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces) MALMSTEN, J. WIDÉN, D. GAVIER RYDEVIK, G. YON, L. HUTCHINGS, M. R. THULIN, C.-G. SÖDERQUIST, L. ASPAN, A. STUEN, S. DALIN, A.-M. 2014-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045167 http://www.ncbi.nlm.nih.gov/pubmed/24001524 https://doi.org/10.1017/S0950268813002094 en eng Cambridge University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24001524 http://dx.doi.org/10.1017/S0950268813002094 © Cambridge University Press 2013 The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence <http://creativecommons.org/licenses/by-nc-sa/3.0/. CC-BY-NC-SA CC-BY Original Papers Text 2014 ftpubmed https://doi.org/10.1017/S0950268813002094 2014-06-08T01:29:50Z The occurrence of Anaplasma phagocytophilum was investigated in spleen and serum samples from Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples were analysed for presence of A. phagocytophilum DNA by real-time PCR (n = 263), and for Anaplasma antibodies with ELISA serology (n = 234). All serum samples had antibodies against A. phagocytophilum. The mean DNA-based prevalence was 26·3%, and significant (P < 0·01) temporal, and spatial variation was found. Island moose had significantly (P < 0·001) higher prevalence of A. phagocytophilum DNA than moose from the mainland areas. Two samples were sequenced to determine genetic variation in the 16S rRNA and groESL genes. Genetic sequence similarity with the human granulocytic anaplasmosis agent, equine granulocytic ehrlichiosis agent, and different wildlife-associated A. phagocytophilum variants were observed in the 16S rRNA and groESL genes. Our study shows that moose are exposed to A. phagocytophilum in Sweden, and represent a potential wildlife reservoir of the pathogen. Text Alces alces PubMed Central (PMC) Found Island ENVELOPE(-115.553,-115.553,61.650,61.650) Epidemiology and Infection 142 6 1205 1213 |
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ftpubmed |
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English |
topic |
Original Papers |
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Original Papers MALMSTEN, J. WIDÉN, D. GAVIER RYDEVIK, G. YON, L. HUTCHINGS, M. R. THULIN, C.-G. SÖDERQUIST, L. ASPAN, A. STUEN, S. DALIN, A.-M. Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces) |
topic_facet |
Original Papers |
description |
The occurrence of Anaplasma phagocytophilum was investigated in spleen and serum samples from Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples were analysed for presence of A. phagocytophilum DNA by real-time PCR (n = 263), and for Anaplasma antibodies with ELISA serology (n = 234). All serum samples had antibodies against A. phagocytophilum. The mean DNA-based prevalence was 26·3%, and significant (P < 0·01) temporal, and spatial variation was found. Island moose had significantly (P < 0·001) higher prevalence of A. phagocytophilum DNA than moose from the mainland areas. Two samples were sequenced to determine genetic variation in the 16S rRNA and groESL genes. Genetic sequence similarity with the human granulocytic anaplasmosis agent, equine granulocytic ehrlichiosis agent, and different wildlife-associated A. phagocytophilum variants were observed in the 16S rRNA and groESL genes. Our study shows that moose are exposed to A. phagocytophilum in Sweden, and represent a potential wildlife reservoir of the pathogen. |
format |
Text |
author |
MALMSTEN, J. WIDÉN, D. GAVIER RYDEVIK, G. YON, L. HUTCHINGS, M. R. THULIN, C.-G. SÖDERQUIST, L. ASPAN, A. STUEN, S. DALIN, A.-M. |
author_facet |
MALMSTEN, J. WIDÉN, D. GAVIER RYDEVIK, G. YON, L. HUTCHINGS, M. R. THULIN, C.-G. SÖDERQUIST, L. ASPAN, A. STUEN, S. DALIN, A.-M. |
author_sort |
MALMSTEN, J. |
title |
Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces) |
title_short |
Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces) |
title_full |
Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces) |
title_fullStr |
Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces) |
title_full_unstemmed |
Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces) |
title_sort |
temporal and spatial variation in anaplasma phagocytophilum infection in swedish moose (alces alces) |
publisher |
Cambridge University Press |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045167 http://www.ncbi.nlm.nih.gov/pubmed/24001524 https://doi.org/10.1017/S0950268813002094 |
long_lat |
ENVELOPE(-115.553,-115.553,61.650,61.650) |
geographic |
Found Island |
geographic_facet |
Found Island |
genre |
Alces alces |
genre_facet |
Alces alces |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24001524 http://dx.doi.org/10.1017/S0950268813002094 |
op_rights |
© Cambridge University Press 2013 The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence <http://creativecommons.org/licenses/by-nc-sa/3.0/. |
op_rightsnorm |
CC-BY-NC-SA CC-BY |
op_doi |
https://doi.org/10.1017/S0950268813002094 |
container_title |
Epidemiology and Infection |
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142 |
container_issue |
6 |
container_start_page |
1205 |
op_container_end_page |
1213 |
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1766254076158279680 |