Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean†
Since their initial discovery in samples from the north Atlantic Ocean, 16S rRNA genes related to the environmental gene clone cluster known as SAR202 have been recovered from pelagic freshwater, marine sediment, soil, and deep subsurface terrestrial environments. Together, these clones form a major...
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ftpubmed:oai:pubmedcentral.nih.gov:404461 2023-05-15T17:30:55+02:00 Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean† Morris, R. M. Rappé, M. S. Urbach, E. Connon, S. A. Giovannoni, S. J. 2004-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC404461 http://www.ncbi.nlm.nih.gov/pubmed/15128540 https://doi.org/10.1128/AEM.70.5.2836-2842.2004 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC404461 http://www.ncbi.nlm.nih.gov/pubmed/15128540 http://dx.doi.org/10.1128/AEM.70.5.2836-2842.2004 Copyright © 2004, American Society for Microbiology Microbial Ecology Text 2004 ftpubmed https://doi.org/10.1128/AEM.70.5.2836-2842.2004 2013-08-29T23:14:47Z Since their initial discovery in samples from the north Atlantic Ocean, 16S rRNA genes related to the environmental gene clone cluster known as SAR202 have been recovered from pelagic freshwater, marine sediment, soil, and deep subsurface terrestrial environments. Together, these clones form a major, monophyletic subgroup of the phylum Chloroflexi. While members of this diverse group are consistently identified in the marine environment, there are currently no cultured representatives, and very little is known about their distribution or abundance in the world's oceans. In this study, published and newly identified SAR202-related 16S rRNA gene sequences were used to further resolve the phylogeny of this cluster and to design taxon-specific oligonucleotide probes for fluorescence in situ hybridization. Direct cell counts from the Bermuda Atlantic time series study site in the north Atlantic Ocean, the Hawaii ocean time series site in the central Pacific Ocean, and along the Newport hydroline in eastern Pacific coastal waters showed that SAR202 cluster cells were most abundant below the deep chlorophyll maximum and that they persisted to 3,600 m in the Atlantic Ocean and to 4,000 m in the Pacific Ocean, the deepest samples used in this study. On average, members of the SAR202 group accounted for 10.2% (±5.7%) of all DNA-containing bacterioplankton between 500 and 4,000 m. Text North Atlantic PubMed Central (PMC) Pacific Applied and Environmental Microbiology 70 5 2836 2842 |
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English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Morris, R. M. Rappé, M. S. Urbach, E. Connon, S. A. Giovannoni, S. J. Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean† |
topic_facet |
Microbial Ecology |
description |
Since their initial discovery in samples from the north Atlantic Ocean, 16S rRNA genes related to the environmental gene clone cluster known as SAR202 have been recovered from pelagic freshwater, marine sediment, soil, and deep subsurface terrestrial environments. Together, these clones form a major, monophyletic subgroup of the phylum Chloroflexi. While members of this diverse group are consistently identified in the marine environment, there are currently no cultured representatives, and very little is known about their distribution or abundance in the world's oceans. In this study, published and newly identified SAR202-related 16S rRNA gene sequences were used to further resolve the phylogeny of this cluster and to design taxon-specific oligonucleotide probes for fluorescence in situ hybridization. Direct cell counts from the Bermuda Atlantic time series study site in the north Atlantic Ocean, the Hawaii ocean time series site in the central Pacific Ocean, and along the Newport hydroline in eastern Pacific coastal waters showed that SAR202 cluster cells were most abundant below the deep chlorophyll maximum and that they persisted to 3,600 m in the Atlantic Ocean and to 4,000 m in the Pacific Ocean, the deepest samples used in this study. On average, members of the SAR202 group accounted for 10.2% (±5.7%) of all DNA-containing bacterioplankton between 500 and 4,000 m. |
format |
Text |
author |
Morris, R. M. Rappé, M. S. Urbach, E. Connon, S. A. Giovannoni, S. J. |
author_facet |
Morris, R. M. Rappé, M. S. Urbach, E. Connon, S. A. Giovannoni, S. J. |
author_sort |
Morris, R. M. |
title |
Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean† |
title_short |
Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean† |
title_full |
Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean† |
title_fullStr |
Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean† |
title_full_unstemmed |
Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean† |
title_sort |
prevalence of the chloroflexi-related sar202 bacterioplankton cluster throughout the mesopelagic zone and deep ocean† |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC404461 http://www.ncbi.nlm.nih.gov/pubmed/15128540 https://doi.org/10.1128/AEM.70.5.2836-2842.2004 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC404461 http://www.ncbi.nlm.nih.gov/pubmed/15128540 http://dx.doi.org/10.1128/AEM.70.5.2836-2842.2004 |
op_rights |
Copyright © 2004, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.70.5.2836-2842.2004 |
container_title |
Applied and Environmental Microbiology |
container_volume |
70 |
container_issue |
5 |
container_start_page |
2836 |
op_container_end_page |
2842 |
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1766128049128996864 |