Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula
Heterotrophic bacteria are well known to be key players in the turnover of dissolved organic material (DOM) in the oceans, but the relationship between DOM uptake and bacterial clades is still not well understood. Here we explore the turnover and single-cell use of glucose, an amino acid mixture, N-...
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American Society for Microbiology
2014
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018847 http://www.ncbi.nlm.nih.gov/pubmed/24657855 https://doi.org/10.1128/AEM.00121-14 |
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ftpubmed:oai:pubmedcentral.nih.gov:4018847 2023-05-15T13:49:18+02:00 Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula Nikrad, Mrinalini P. Cottrell, Matthew T. Kirchman, David L. 2014-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018847 http://www.ncbi.nlm.nih.gov/pubmed/24657855 https://doi.org/10.1128/AEM.00121-14 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24657855 http://dx.doi.org/10.1128/AEM.00121-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved. Microbial Ecology Text 2014 ftpubmed https://doi.org/10.1128/AEM.00121-14 2014-12-07T01:15:10Z Heterotrophic bacteria are well known to be key players in the turnover of dissolved organic material (DOM) in the oceans, but the relationship between DOM uptake and bacterial clades is still not well understood. Here we explore the turnover and single-cell use of glucose, an amino acid mixture, N-acetylglucosamine (NAG), and protein by gammaproteobacterial clades in coastal waters of the West Antarctic Peninsula in summer and fall. More than 60% of the cells within two closely related gammaproteobacterial clades, Ant4D3 and Arctic96B-16, were active in using the amino acid mixture, protein, and NAG. In contrast, an average of only 7% of all SAR86 cells used amino acids and protein even in summer when DOM use was high. In addition to DOM uptake within a group, we explored the contribution of the three gammaproteobacterial groups to total community uptake of a compound. SAR86 contributed 5- to 10-fold less than the other gammaproteobacterial subgroups to the uptake of all compounds. We found that the overall contribution of the Ant4D3 clade to DOM uptake was highest, whereas the SAR86 clade contributed the least to DOM turnover in West Antarctic Peninsula waters. Our results suggest that the low growth activity of a bacterial clade leads to low abundance, fewer active cells and a low contribution to the turnover of DOM components. Text Antarc* Antarctic Antarctic Peninsula PubMed Central (PMC) Antarctic Antarctic Peninsula Applied and Environmental Microbiology 80 11 3362 3368 |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Nikrad, Mrinalini P. Cottrell, Matthew T. Kirchman, David L. Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
topic_facet |
Microbial Ecology |
description |
Heterotrophic bacteria are well known to be key players in the turnover of dissolved organic material (DOM) in the oceans, but the relationship between DOM uptake and bacterial clades is still not well understood. Here we explore the turnover and single-cell use of glucose, an amino acid mixture, N-acetylglucosamine (NAG), and protein by gammaproteobacterial clades in coastal waters of the West Antarctic Peninsula in summer and fall. More than 60% of the cells within two closely related gammaproteobacterial clades, Ant4D3 and Arctic96B-16, were active in using the amino acid mixture, protein, and NAG. In contrast, an average of only 7% of all SAR86 cells used amino acids and protein even in summer when DOM use was high. In addition to DOM uptake within a group, we explored the contribution of the three gammaproteobacterial groups to total community uptake of a compound. SAR86 contributed 5- to 10-fold less than the other gammaproteobacterial subgroups to the uptake of all compounds. We found that the overall contribution of the Ant4D3 clade to DOM uptake was highest, whereas the SAR86 clade contributed the least to DOM turnover in West Antarctic Peninsula waters. Our results suggest that the low growth activity of a bacterial clade leads to low abundance, fewer active cells and a low contribution to the turnover of DOM components. |
format |
Text |
author |
Nikrad, Mrinalini P. Cottrell, Matthew T. Kirchman, David L. |
author_facet |
Nikrad, Mrinalini P. Cottrell, Matthew T. Kirchman, David L. |
author_sort |
Nikrad, Mrinalini P. |
title |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_short |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_full |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_fullStr |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_full_unstemmed |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_sort |
uptake of dissolved organic carbon by gammaproteobacterial subgroups in coastal waters of the west antarctic peninsula |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018847 http://www.ncbi.nlm.nih.gov/pubmed/24657855 https://doi.org/10.1128/AEM.00121-14 |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24657855 http://dx.doi.org/10.1128/AEM.00121-14 |
op_rights |
Copyright © 2014, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.00121-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
11 |
container_start_page |
3362 |
op_container_end_page |
3368 |
_version_ |
1766251145679863808 |