The Relationship between the Structure of the Tick-Borne Encephalitis Virus Strains and Their Pathogenic Properties
Tick-borne encephalitis virus (TBEV) is transmitted to vertebrates by taiga or forest ticks through bites, inducing disease of variable severity. The reasons underlying these differences in the severity of the disease are unknown. In order to identify genetic factors affecting the pathogenicity of v...
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ftpubmed:oai:pubmedcentral.nih.gov:3989262 2023-05-15T18:30:59+02:00 The Relationship between the Structure of the Tick-Borne Encephalitis Virus Strains and Their Pathogenic Properties Belikov, Sergei I. Kondratov, Ilya G. Potapova, Ulyana V. Leonova, Galina N. 2014-04-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989262 http://www.ncbi.nlm.nih.gov/pubmed/24740396 https://doi.org/10.1371/journal.pone.0094946 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989262 http://www.ncbi.nlm.nih.gov/pubmed/24740396 http://dx.doi.org/10.1371/journal.pone.0094946 This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2014 ftpubmed https://doi.org/10.1371/journal.pone.0094946 2014-04-27T00:42:16Z Tick-borne encephalitis virus (TBEV) is transmitted to vertebrates by taiga or forest ticks through bites, inducing disease of variable severity. The reasons underlying these differences in the severity of the disease are unknown. In order to identify genetic factors affecting the pathogenicity of virus strains, we have sequenced and compared the complete genomes of 34 Far-Eastern subtype (FE) TBEV strains isolated from patients with different disease severity (Primorye, the Russian Far East). We analyzed the complete genomes of 11 human pathogenic strains isolated from the brains of dead patients with the encephalitic form of the disease (Efd), 4 strains from the blood of patients with the febrile form of TBE (Ffd), and 19 strains from patients with the subclinical form of TBE (Sfd). On the phylogenetic tree, pathogenic Efd strains formed two clusters containing the prototype strains, Senzhang and Sofjin, respectively. Sfd strains formed a third separate cluster, including the Oshima strain. The strains that caused the febrile form of the disease did not form a separate cluster. In the viral proteins, we found 198 positions with at least one amino acid residue substitution, of which only 17 amino acid residue substitutions were correlated with the variable pathogenicity of these strains in humans and they authentically differed between the groups. We considered the role of each amino acid substitution and assumed that the deletion of 111 amino acids in the capsid protein in combination with the amino acid substitutions R16K and S45F in the NS3 protease may affect the budding process of viral particles. These changes may be the major reason for the diminished pathogenicity of TBEV strains. We recommend Sfd strains for testing as attenuation vaccine candidates. Text taiga PubMed Central (PMC) PLoS ONE 9 4 e94946 |
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Research Article Belikov, Sergei I. Kondratov, Ilya G. Potapova, Ulyana V. Leonova, Galina N. The Relationship between the Structure of the Tick-Borne Encephalitis Virus Strains and Their Pathogenic Properties |
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Research Article |
description |
Tick-borne encephalitis virus (TBEV) is transmitted to vertebrates by taiga or forest ticks through bites, inducing disease of variable severity. The reasons underlying these differences in the severity of the disease are unknown. In order to identify genetic factors affecting the pathogenicity of virus strains, we have sequenced and compared the complete genomes of 34 Far-Eastern subtype (FE) TBEV strains isolated from patients with different disease severity (Primorye, the Russian Far East). We analyzed the complete genomes of 11 human pathogenic strains isolated from the brains of dead patients with the encephalitic form of the disease (Efd), 4 strains from the blood of patients with the febrile form of TBE (Ffd), and 19 strains from patients with the subclinical form of TBE (Sfd). On the phylogenetic tree, pathogenic Efd strains formed two clusters containing the prototype strains, Senzhang and Sofjin, respectively. Sfd strains formed a third separate cluster, including the Oshima strain. The strains that caused the febrile form of the disease did not form a separate cluster. In the viral proteins, we found 198 positions with at least one amino acid residue substitution, of which only 17 amino acid residue substitutions were correlated with the variable pathogenicity of these strains in humans and they authentically differed between the groups. We considered the role of each amino acid substitution and assumed that the deletion of 111 amino acids in the capsid protein in combination with the amino acid substitutions R16K and S45F in the NS3 protease may affect the budding process of viral particles. These changes may be the major reason for the diminished pathogenicity of TBEV strains. We recommend Sfd strains for testing as attenuation vaccine candidates. |
format |
Text |
author |
Belikov, Sergei I. Kondratov, Ilya G. Potapova, Ulyana V. Leonova, Galina N. |
author_facet |
Belikov, Sergei I. Kondratov, Ilya G. Potapova, Ulyana V. Leonova, Galina N. |
author_sort |
Belikov, Sergei I. |
title |
The Relationship between the Structure of the Tick-Borne Encephalitis Virus Strains and Their Pathogenic Properties |
title_short |
The Relationship between the Structure of the Tick-Borne Encephalitis Virus Strains and Their Pathogenic Properties |
title_full |
The Relationship between the Structure of the Tick-Borne Encephalitis Virus Strains and Their Pathogenic Properties |
title_fullStr |
The Relationship between the Structure of the Tick-Borne Encephalitis Virus Strains and Their Pathogenic Properties |
title_full_unstemmed |
The Relationship between the Structure of the Tick-Borne Encephalitis Virus Strains and Their Pathogenic Properties |
title_sort |
relationship between the structure of the tick-borne encephalitis virus strains and their pathogenic properties |
publisher |
Public Library of Science |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989262 http://www.ncbi.nlm.nih.gov/pubmed/24740396 https://doi.org/10.1371/journal.pone.0094946 |
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taiga |
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taiga |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989262 http://www.ncbi.nlm.nih.gov/pubmed/24740396 http://dx.doi.org/10.1371/journal.pone.0094946 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0094946 |
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PLoS ONE |
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9 |
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4 |
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e94946 |
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