Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic...
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ftpubmed:oai:pubmedcentral.nih.gov:3983602 2023-05-15T14:47:49+02:00 Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean Pedneault, Estelle Galand, Pierre E. Potvin, Marianne Tremblay, Jean-Éric Lovejoy, Connie 2014-04-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983602 https://doi.org/10.1038/srep04661 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983602 http://dx.doi.org/10.1038/srep04661 Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ CC-BY-NC-ND Article Text 2014 ftpubmed https://doi.org/10.1038/srep04661 2014-04-13T00:57:02Z Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean. Text Arctic Arctic Ocean PubMed Central (PMC) Arctic Arctic Ocean Scientific Reports 4 1 |
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Article Pedneault, Estelle Galand, Pierre E. Potvin, Marianne Tremblay, Jean-Éric Lovejoy, Connie Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
topic_facet |
Article |
description |
Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean. |
format |
Text |
author |
Pedneault, Estelle Galand, Pierre E. Potvin, Marianne Tremblay, Jean-Éric Lovejoy, Connie |
author_facet |
Pedneault, Estelle Galand, Pierre E. Potvin, Marianne Tremblay, Jean-Éric Lovejoy, Connie |
author_sort |
Pedneault, Estelle |
title |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_short |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_full |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_fullStr |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_full_unstemmed |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_sort |
archaeal amoa and urec genes and their transcriptional activity in the arctic ocean |
publisher |
Nature Publishing Group |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983602 https://doi.org/10.1038/srep04661 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983602 http://dx.doi.org/10.1038/srep04661 |
op_rights |
Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
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CC-BY-NC-ND |
op_doi |
https://doi.org/10.1038/srep04661 |
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Scientific Reports |
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4 |
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1 |
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1766318935694639104 |