Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens
We tested sera from 286 agricultural workers and 322 rodents in the department of Córdoba, northeastern Colombia, for antibodies against two hantaviruses. The sera were analysed by indirect ELISA using the lysate of Vero E6 cells infected with Maciel virus (MACV) or the N protein of Araraquara virus...
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ftpubmed:oai:pubmedcentral.nih.gov:3970671 2023-05-15T18:05:28+02:00 Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens Guzmán, Camilo Mattar, Salim Levis, Silvana Pini, Noemí Figueiredo, Tadeu Mills, James Salazar-Bravo, Jorge 2013-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970671 http://www.ncbi.nlm.nih.gov/pubmed/23579795 https://doi.org/10.1590/0074-0276108022013007 en eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23579795 http://dx.doi.org/10.1590/0074-0276108022013007 Articles Text 2013 ftpubmed https://doi.org/10.1590/0074-0276108022013007 2014-05-25T00:33:03Z We tested sera from 286 agricultural workers and 322 rodents in the department of Córdoba, northeastern Colombia, for antibodies against two hantaviruses. The sera were analysed by indirect ELISA using the lysate of Vero E6 cells infected with Maciel virus (MACV) or the N protein of Araraquara virus (ARAV) as antigens for the detection of antibodies against hantaviruses. Twenty-four human sera were IgG positive using one or both antigens. We detected anti-MACV IgG antibodies in 10 sera (3.5%) and anti-ARAV antibodies in 21 sera (7.34%). Of the 10 samples that were positive for MACV, seven (70%) were cross-reactive with ARAV; seven of the 21 ARAV-positive samples were cross-reactive with MACV. Using an ARAV IgM ELISA, two of the 24 human sera (8.4%) were positive. We captured 322 rodents, including 210 Cricetidae (181 Zygodontomys brevicauda, 28 Oligoryzomys fulvescens and 1 Oecomys trinitatis), six Heteromys anomalus (Heteromyidae), one Proechimys sp. (Echimyidae) and 105 Muridae (34 Rattus rattus and 71 Mus musculus). All rodent sera were negative for both antigens. The 8.4% detection rate of hantavirus antibodies in humans is much higher than previously found in serosurveys in North America, suggesting that rural agricultural workers in northeastern Colombia are frequently exposed to hantaviruses. Our results also indicate that tests conducted with South American hantavirus antigens could have predictive value and could represent a useful alternative for the diagnosis of hantavirus infection in Colombia. Text Rattus rattus PubMed Central (PMC) Maciel ENVELOPE(-67.850,-67.850,-66.833,-66.833) Memórias do Instituto Oswaldo Cruz 108 2 167 171 |
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Articles Guzmán, Camilo Mattar, Salim Levis, Silvana Pini, Noemí Figueiredo, Tadeu Mills, James Salazar-Bravo, Jorge Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
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description |
We tested sera from 286 agricultural workers and 322 rodents in the department of Córdoba, northeastern Colombia, for antibodies against two hantaviruses. The sera were analysed by indirect ELISA using the lysate of Vero E6 cells infected with Maciel virus (MACV) or the N protein of Araraquara virus (ARAV) as antigens for the detection of antibodies against hantaviruses. Twenty-four human sera were IgG positive using one or both antigens. We detected anti-MACV IgG antibodies in 10 sera (3.5%) and anti-ARAV antibodies in 21 sera (7.34%). Of the 10 samples that were positive for MACV, seven (70%) were cross-reactive with ARAV; seven of the 21 ARAV-positive samples were cross-reactive with MACV. Using an ARAV IgM ELISA, two of the 24 human sera (8.4%) were positive. We captured 322 rodents, including 210 Cricetidae (181 Zygodontomys brevicauda, 28 Oligoryzomys fulvescens and 1 Oecomys trinitatis), six Heteromys anomalus (Heteromyidae), one Proechimys sp. (Echimyidae) and 105 Muridae (34 Rattus rattus and 71 Mus musculus). All rodent sera were negative for both antigens. The 8.4% detection rate of hantavirus antibodies in humans is much higher than previously found in serosurveys in North America, suggesting that rural agricultural workers in northeastern Colombia are frequently exposed to hantaviruses. Our results also indicate that tests conducted with South American hantavirus antigens could have predictive value and could represent a useful alternative for the diagnosis of hantavirus infection in Colombia. |
format |
Text |
author |
Guzmán, Camilo Mattar, Salim Levis, Silvana Pini, Noemí Figueiredo, Tadeu Mills, James Salazar-Bravo, Jorge |
author_facet |
Guzmán, Camilo Mattar, Salim Levis, Silvana Pini, Noemí Figueiredo, Tadeu Mills, James Salazar-Bravo, Jorge |
author_sort |
Guzmán, Camilo |
title |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_short |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_full |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_fullStr |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_full_unstemmed |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_sort |
prevalence of antibody to hantaviruses in humans and rodents in the caribbean region of colombia determined using araraquara and maciel virus antigens |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970671 http://www.ncbi.nlm.nih.gov/pubmed/23579795 https://doi.org/10.1590/0074-0276108022013007 |
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ENVELOPE(-67.850,-67.850,-66.833,-66.833) |
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Maciel |
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Maciel |
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Rattus rattus |
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Rattus rattus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23579795 http://dx.doi.org/10.1590/0074-0276108022013007 |
op_doi |
https://doi.org/10.1590/0074-0276108022013007 |
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Memórias do Instituto Oswaldo Cruz |
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108 |
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2 |
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167 |
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171 |
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