Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay
Using 16S rRNA gene sequencing analysis, we examined the bacterial diversity and the presence of opportunistic bacterial pathogens (i.e., Campylobacter and Helicobacter) in red knot (Calidris canutus; n = 40), ruddy turnstone (Arenaria interpres; n = 35), and semipalmated sandpiper (Calidris pusilla...
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ftpubmed:oai:pubmedcentral.nih.gov:3957654 2023-05-15T15:23:18+02:00 Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay Ryu, Hodon Grond, Kirsten Verheijen, Bram Elk, Michael Buehler, Deborah M. Santo Domingo, Jorge W. 2014-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957654 http://www.ncbi.nlm.nih.gov/pubmed/24413599 https://doi.org/10.1128/AEM.03793-13 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24413599 http://dx.doi.org/10.1128/AEM.03793-13 Copyright © 2014, American Society for Microbiology. All Rights Reserved. Public and Environmental Health Microbiology Text 2014 ftpubmed https://doi.org/10.1128/AEM.03793-13 2014-09-07T01:11:07Z Using 16S rRNA gene sequencing analysis, we examined the bacterial diversity and the presence of opportunistic bacterial pathogens (i.e., Campylobacter and Helicobacter) in red knot (Calidris canutus; n = 40), ruddy turnstone (Arenaria interpres; n = 35), and semipalmated sandpiper (Calidris pusilla; n = 22) fecal samples collected during a migratory stopover in Delaware Bay. Additionally, we studied the occurrence of Campylobacter spp., enterococci, and waterfowl fecal source markers using quantitative PCR (qPCR) assays. Of 3,889 16S rRNA clone sequences analyzed, the bacterial community was mostly composed of Bacilli (63.5%), Fusobacteria (12.7%), Epsilonproteobacteria (6.5%), and Clostridia (5.8%). When epsilonproteobacterium-specific 23S rRNA gene clone libraries (i.e., 1,414 sequences) were analyzed, the sequences were identified as Campylobacter (82.3%) or Helicobacter (17.7%) spp. Specifically, 38.4%, 10.1%, and 26.0% of clone sequences were identified as C. lari (>99% sequence identity) in ruddy turnstone, red knot, and semipalmated sandpiper clone libraries, respectively. Other pathogenic species of Campylobacter, such as C. jejuni and C. coli, were not detected in excreta of any of the three bird species. Most Helicobacter-like sequences identified were closely related to H. pametensis (>99% sequence identity) and H. anseris (92% sequence identity). qPCR results showed that the occurrence and abundance of Campylobacter spp. was relatively high compared to those of fecal indicator bacteria, such as Enterococcus spp., E. faecalis, and Catellicoccus marimammalium. Overall, the results provide insights into the complexity of the shorebird gut microbial community and suggest that these migratory birds are important reservoirs of pathogenic Campylobacter species. Text Arenaria interpres Calidris canutus Red Knot Ruddy Turnstone PubMed Central (PMC) Applied and Environmental Microbiology 80 6 1838 1847 |
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Public and Environmental Health Microbiology |
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Public and Environmental Health Microbiology Ryu, Hodon Grond, Kirsten Verheijen, Bram Elk, Michael Buehler, Deborah M. Santo Domingo, Jorge W. Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay |
topic_facet |
Public and Environmental Health Microbiology |
description |
Using 16S rRNA gene sequencing analysis, we examined the bacterial diversity and the presence of opportunistic bacterial pathogens (i.e., Campylobacter and Helicobacter) in red knot (Calidris canutus; n = 40), ruddy turnstone (Arenaria interpres; n = 35), and semipalmated sandpiper (Calidris pusilla; n = 22) fecal samples collected during a migratory stopover in Delaware Bay. Additionally, we studied the occurrence of Campylobacter spp., enterococci, and waterfowl fecal source markers using quantitative PCR (qPCR) assays. Of 3,889 16S rRNA clone sequences analyzed, the bacterial community was mostly composed of Bacilli (63.5%), Fusobacteria (12.7%), Epsilonproteobacteria (6.5%), and Clostridia (5.8%). When epsilonproteobacterium-specific 23S rRNA gene clone libraries (i.e., 1,414 sequences) were analyzed, the sequences were identified as Campylobacter (82.3%) or Helicobacter (17.7%) spp. Specifically, 38.4%, 10.1%, and 26.0% of clone sequences were identified as C. lari (>99% sequence identity) in ruddy turnstone, red knot, and semipalmated sandpiper clone libraries, respectively. Other pathogenic species of Campylobacter, such as C. jejuni and C. coli, were not detected in excreta of any of the three bird species. Most Helicobacter-like sequences identified were closely related to H. pametensis (>99% sequence identity) and H. anseris (92% sequence identity). qPCR results showed that the occurrence and abundance of Campylobacter spp. was relatively high compared to those of fecal indicator bacteria, such as Enterococcus spp., E. faecalis, and Catellicoccus marimammalium. Overall, the results provide insights into the complexity of the shorebird gut microbial community and suggest that these migratory birds are important reservoirs of pathogenic Campylobacter species. |
format |
Text |
author |
Ryu, Hodon Grond, Kirsten Verheijen, Bram Elk, Michael Buehler, Deborah M. Santo Domingo, Jorge W. |
author_facet |
Ryu, Hodon Grond, Kirsten Verheijen, Bram Elk, Michael Buehler, Deborah M. Santo Domingo, Jorge W. |
author_sort |
Ryu, Hodon |
title |
Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay |
title_short |
Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay |
title_full |
Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay |
title_fullStr |
Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay |
title_full_unstemmed |
Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay |
title_sort |
intestinal microbiota and species diversity of campylobacter and helicobacter spp. in migrating shorebirds in delaware bay |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957654 http://www.ncbi.nlm.nih.gov/pubmed/24413599 https://doi.org/10.1128/AEM.03793-13 |
genre |
Arenaria interpres Calidris canutus Red Knot Ruddy Turnstone |
genre_facet |
Arenaria interpres Calidris canutus Red Knot Ruddy Turnstone |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24413599 http://dx.doi.org/10.1128/AEM.03793-13 |
op_rights |
Copyright © 2014, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.03793-13 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
6 |
container_start_page |
1838 |
op_container_end_page |
1847 |
_version_ |
1766354014177329152 |