Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics
While microbial communities play a key role in the geochemical cycling of nutrients and contaminants in anaerobic freshwater sediments, their structure and activity in polar desert ecosystems are still poorly understood, both across heterogeneous freshwater environments such as lakes and wetlands, a...
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ftpubmed:oai:pubmedcentral.nih.gov:3940601 2023-05-15T15:08:34+02:00 Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics Stoeva, Magdalena K. Aris-Brosou, Stéphane Chételat, John Hintelmann, Holger Pelletier, Philip Poulain, Alexandre J. 2014-03-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940601 http://www.ncbi.nlm.nih.gov/pubmed/24594936 https://doi.org/10.1371/journal.pone.0089531 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940601 http://www.ncbi.nlm.nih.gov/pubmed/24594936 http://dx.doi.org/10.1371/journal.pone.0089531 This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2014 ftpubmed https://doi.org/10.1371/journal.pone.0089531 2014-03-09T01:52:49Z While microbial communities play a key role in the geochemical cycling of nutrients and contaminants in anaerobic freshwater sediments, their structure and activity in polar desert ecosystems are still poorly understood, both across heterogeneous freshwater environments such as lakes and wetlands, and across sediment depths. To address this question, we performed targeted environmental transcriptomics analyses and characterized microbial diversity across three depths from sediment cores collected in a lake and a wetland, located on Cornwallis Island, NU, Canada. Microbial communities were characterized based on 16S rRNA and two functional gene transcripts: mcrA, involved in archaeal methane cycling and glnA, a bacterial housekeeping gene implicated in nitrogen metabolism. We show that methane cycling and overall bacterial metabolic activity are the highest at the surface of lake sediments but deeper within wetland sediments. Bacterial communities are highly diverse and structured as a function of both environment and depth, being more diverse in the wetland and near the surface. Archaea are mostly methanogens, structured by environment and more diverse in the wetland. McrA transcript analyses show that active methane cycling in the lake and wetland corresponds to distinct communities with a higher potential for methane cycling in the wetland. Methanosarcina spp., Methanosaeta spp. and a group of uncultured Archaea are the dominant methanogens in the wetland while Methanoregula spp. predominate in the lake. Text Arctic Cornwallis Island polar desert PubMed Central (PMC) Arctic Canada Cornwallis ENVELOPE(-54.464,-54.464,-61.072,-61.072) Cornwallis Island ENVELOPE(-95.001,-95.001,75.135,75.135) PLoS ONE 9 3 e89531 |
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English |
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Research Article |
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Research Article Stoeva, Magdalena K. Aris-Brosou, Stéphane Chételat, John Hintelmann, Holger Pelletier, Philip Poulain, Alexandre J. Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics |
topic_facet |
Research Article |
description |
While microbial communities play a key role in the geochemical cycling of nutrients and contaminants in anaerobic freshwater sediments, their structure and activity in polar desert ecosystems are still poorly understood, both across heterogeneous freshwater environments such as lakes and wetlands, and across sediment depths. To address this question, we performed targeted environmental transcriptomics analyses and characterized microbial diversity across three depths from sediment cores collected in a lake and a wetland, located on Cornwallis Island, NU, Canada. Microbial communities were characterized based on 16S rRNA and two functional gene transcripts: mcrA, involved in archaeal methane cycling and glnA, a bacterial housekeeping gene implicated in nitrogen metabolism. We show that methane cycling and overall bacterial metabolic activity are the highest at the surface of lake sediments but deeper within wetland sediments. Bacterial communities are highly diverse and structured as a function of both environment and depth, being more diverse in the wetland and near the surface. Archaea are mostly methanogens, structured by environment and more diverse in the wetland. McrA transcript analyses show that active methane cycling in the lake and wetland corresponds to distinct communities with a higher potential for methane cycling in the wetland. Methanosarcina spp., Methanosaeta spp. and a group of uncultured Archaea are the dominant methanogens in the wetland while Methanoregula spp. predominate in the lake. |
format |
Text |
author |
Stoeva, Magdalena K. Aris-Brosou, Stéphane Chételat, John Hintelmann, Holger Pelletier, Philip Poulain, Alexandre J. |
author_facet |
Stoeva, Magdalena K. Aris-Brosou, Stéphane Chételat, John Hintelmann, Holger Pelletier, Philip Poulain, Alexandre J. |
author_sort |
Stoeva, Magdalena K. |
title |
Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics |
title_short |
Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics |
title_full |
Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics |
title_fullStr |
Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics |
title_full_unstemmed |
Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics |
title_sort |
microbial community structure in lake and wetland sediments from a high arctic polar desert revealed by targeted transcriptomics |
publisher |
Public Library of Science |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940601 http://www.ncbi.nlm.nih.gov/pubmed/24594936 https://doi.org/10.1371/journal.pone.0089531 |
long_lat |
ENVELOPE(-54.464,-54.464,-61.072,-61.072) ENVELOPE(-95.001,-95.001,75.135,75.135) |
geographic |
Arctic Canada Cornwallis Cornwallis Island |
geographic_facet |
Arctic Canada Cornwallis Cornwallis Island |
genre |
Arctic Cornwallis Island polar desert |
genre_facet |
Arctic Cornwallis Island polar desert |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940601 http://www.ncbi.nlm.nih.gov/pubmed/24594936 http://dx.doi.org/10.1371/journal.pone.0089531 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0089531 |
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PLoS ONE |
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9 |
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3 |
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e89531 |
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