First Molecular Evidence for Puumala Hantavirus in Poland
Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European...
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ftpubmed:oai:pubmedcentral.nih.gov:3917447 2023-05-15T16:11:58+02:00 First Molecular Evidence for Puumala Hantavirus in Poland Sheikh Ali, Hanan Drewes, Stephan Sadowska, Edyta T. Mikowska, Magdalena Groschup, Martin H. Heckel, Gerald Koteja, Pawel Ulrich, Rainer G. 2014-01-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917447 http://www.ncbi.nlm.nih.gov/pubmed/24452006 https://doi.org/10.3390/v6010340 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917447 http://www.ncbi.nlm.nih.gov/pubmed/24452006 http://dx.doi.org/10.3390/v6010340 © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). CC-BY Brief Report Text 2014 ftpubmed https://doi.org/10.3390/v6010340 2014-02-09T01:55:08Z Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikołajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikołajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikołajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland. Text Fennoscandia PubMed Central (PMC) Viruses 6 1 340 353 |
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Brief Report |
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Brief Report Sheikh Ali, Hanan Drewes, Stephan Sadowska, Edyta T. Mikowska, Magdalena Groschup, Martin H. Heckel, Gerald Koteja, Pawel Ulrich, Rainer G. First Molecular Evidence for Puumala Hantavirus in Poland |
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Brief Report |
description |
Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikołajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikołajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikołajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland. |
format |
Text |
author |
Sheikh Ali, Hanan Drewes, Stephan Sadowska, Edyta T. Mikowska, Magdalena Groschup, Martin H. Heckel, Gerald Koteja, Pawel Ulrich, Rainer G. |
author_facet |
Sheikh Ali, Hanan Drewes, Stephan Sadowska, Edyta T. Mikowska, Magdalena Groschup, Martin H. Heckel, Gerald Koteja, Pawel Ulrich, Rainer G. |
author_sort |
Sheikh Ali, Hanan |
title |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_short |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_full |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_fullStr |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_full_unstemmed |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_sort |
first molecular evidence for puumala hantavirus in poland |
publisher |
MDPI |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917447 http://www.ncbi.nlm.nih.gov/pubmed/24452006 https://doi.org/10.3390/v6010340 |
genre |
Fennoscandia |
genre_facet |
Fennoscandia |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917447 http://www.ncbi.nlm.nih.gov/pubmed/24452006 http://dx.doi.org/10.3390/v6010340 |
op_rights |
© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/v6010340 |
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Viruses |
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340 |
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353 |
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