Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have b...
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ftpubmed:oai:pubmedcentral.nih.gov:3897894 2023-05-15T15:58:49+02:00 Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens Renault, Tristan Tchaleu, Gwenaëlle Faury, Nicole Moreau, Pierrick Segarra, Amélie Barbosa-Solomieu, Valérie Lapègue, Sylvie 2014 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897894 http://www.ncbi.nlm.nih.gov/pubmed/24410800 https://doi.org/10.1186/1297-9716-45-3 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897894 http://www.ncbi.nlm.nih.gov/pubmed/24410800 http://dx.doi.org/10.1186/1297-9716-45-3 Copyright © 2014 Renault et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Text 2014 ftpubmed https://doi.org/10.1186/1297-9716-45-3 2014-01-26T01:48:07Z Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest. Determination of nucleotide sequences of PCR-amplified virus DNA fragments has already been used to characterize OsHV-1 specimens and virus variants have thus been described. However, the virus DNA sequencing approach is time-consuming in the high-scale format. Identification and genotyping of highly polymorphic microsatellite loci appear as a suitable approach. The main objective of the present study was the development of a genotyping method in order to characterise clinical OsHV-1 specimens by targeting a particular microsatellite locus located in the ORF4 area. Genotyping results were compared to sequences already available. An excellent correlation was found between the detected genotypes and the corresponding sequences showing that the genotyping approach allowed an accuraté discrimination between virus specimens. Text Crassostrea gigas PubMed Central (PMC) Pacific Veterinary Research 45 1 3 |
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Research Renault, Tristan Tchaleu, Gwenaëlle Faury, Nicole Moreau, Pierrick Segarra, Amélie Barbosa-Solomieu, Valérie Lapègue, Sylvie Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens |
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Research |
description |
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest. Determination of nucleotide sequences of PCR-amplified virus DNA fragments has already been used to characterize OsHV-1 specimens and virus variants have thus been described. However, the virus DNA sequencing approach is time-consuming in the high-scale format. Identification and genotyping of highly polymorphic microsatellite loci appear as a suitable approach. The main objective of the present study was the development of a genotyping method in order to characterise clinical OsHV-1 specimens by targeting a particular microsatellite locus located in the ORF4 area. Genotyping results were compared to sequences already available. An excellent correlation was found between the detected genotypes and the corresponding sequences showing that the genotyping approach allowed an accuraté discrimination between virus specimens. |
format |
Text |
author |
Renault, Tristan Tchaleu, Gwenaëlle Faury, Nicole Moreau, Pierrick Segarra, Amélie Barbosa-Solomieu, Valérie Lapègue, Sylvie |
author_facet |
Renault, Tristan Tchaleu, Gwenaëlle Faury, Nicole Moreau, Pierrick Segarra, Amélie Barbosa-Solomieu, Valérie Lapègue, Sylvie |
author_sort |
Renault, Tristan |
title |
Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens |
title_short |
Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens |
title_full |
Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens |
title_fullStr |
Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens |
title_full_unstemmed |
Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens |
title_sort |
genotyping of a microsatellite locus to differentiate clinical ostreid herpesvirus 1 specimens |
publisher |
BioMed Central |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897894 http://www.ncbi.nlm.nih.gov/pubmed/24410800 https://doi.org/10.1186/1297-9716-45-3 |
geographic |
Pacific |
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Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897894 http://www.ncbi.nlm.nih.gov/pubmed/24410800 http://dx.doi.org/10.1186/1297-9716-45-3 |
op_rights |
Copyright © 2014 Renault et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/1297-9716-45-3 |
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Veterinary Research |
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45 |
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