Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic
Permafrost-affected soils are among the most obvious ecosystems in which current microbial controls on organic matter decomposition are changing as a result of global warming. Warmer conditions in polygonal tundra will lead to a deepening of the seasonal active layer, provoking changes in microbial...
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ftpubmed:oai:pubmedcentral.nih.gov:3885591 2023-05-15T14:52:35+02:00 Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic Frank-Fahle, Béatrice A. Yergeau, Étienne Greer, Charles W. Lantuit, Hugues Wagner, Dirk 2014-01-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885591 https://doi.org/10.1371/journal.pone.0084761 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885591 http://dx.doi.org/10.1371/journal.pone.0084761 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2014 ftpubmed https://doi.org/10.1371/journal.pone.0084761 2014-01-12T01:53:18Z Permafrost-affected soils are among the most obvious ecosystems in which current microbial controls on organic matter decomposition are changing as a result of global warming. Warmer conditions in polygonal tundra will lead to a deepening of the seasonal active layer, provoking changes in microbial processes and possibly resulting in exacerbated carbon degradation under increasing anoxic conditions. To identify current microbial assemblages in carbon rich, water saturated permafrost environments, four polygonal tundra sites were investigated on Herschel Island and the Yukon Coast, Western Canadian Arctic. Ion Torrent sequencing of bacterial and archaeal 16S rRNA amplicons revealed the presence of all major microbial soil groups and indicated a local, vertical heterogeneity of the polygonal tundra soil community with increasing depth. Microbial diversity was found to be highest in the surface layers, decreasing towards the permafrost table. Quantitative PCR analysis of functional genes involved in carbon and nitrogen-cycling revealed a high functional potential in the surface layers, decreasing with increasing active layer depth. We observed that soil properties driving microbial diversity and functional potential varied in each study site. These results highlight the small-scale heterogeneity of geomorphologically comparable sites, greatly restricting generalizations about the fate of permafrost-affected environments in a warming Arctic. Text Arctic Global warming Herschel Herschel Island permafrost Tundra Yukon PubMed Central (PMC) Arctic Herschel Island ENVELOPE(-139.089,-139.089,69.583,69.583) Yukon PLoS ONE 9 1 e84761 |
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Research Article Frank-Fahle, Béatrice A. Yergeau, Étienne Greer, Charles W. Lantuit, Hugues Wagner, Dirk Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
topic_facet |
Research Article |
description |
Permafrost-affected soils are among the most obvious ecosystems in which current microbial controls on organic matter decomposition are changing as a result of global warming. Warmer conditions in polygonal tundra will lead to a deepening of the seasonal active layer, provoking changes in microbial processes and possibly resulting in exacerbated carbon degradation under increasing anoxic conditions. To identify current microbial assemblages in carbon rich, water saturated permafrost environments, four polygonal tundra sites were investigated on Herschel Island and the Yukon Coast, Western Canadian Arctic. Ion Torrent sequencing of bacterial and archaeal 16S rRNA amplicons revealed the presence of all major microbial soil groups and indicated a local, vertical heterogeneity of the polygonal tundra soil community with increasing depth. Microbial diversity was found to be highest in the surface layers, decreasing towards the permafrost table. Quantitative PCR analysis of functional genes involved in carbon and nitrogen-cycling revealed a high functional potential in the surface layers, decreasing with increasing active layer depth. We observed that soil properties driving microbial diversity and functional potential varied in each study site. These results highlight the small-scale heterogeneity of geomorphologically comparable sites, greatly restricting generalizations about the fate of permafrost-affected environments in a warming Arctic. |
format |
Text |
author |
Frank-Fahle, Béatrice A. Yergeau, Étienne Greer, Charles W. Lantuit, Hugues Wagner, Dirk |
author_facet |
Frank-Fahle, Béatrice A. Yergeau, Étienne Greer, Charles W. Lantuit, Hugues Wagner, Dirk |
author_sort |
Frank-Fahle, Béatrice A. |
title |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_short |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_full |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_fullStr |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_full_unstemmed |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_sort |
microbial functional potential and community composition in permafrost-affected soils of the nw canadian arctic |
publisher |
Public Library of Science |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885591 https://doi.org/10.1371/journal.pone.0084761 |
long_lat |
ENVELOPE(-139.089,-139.089,69.583,69.583) |
geographic |
Arctic Herschel Island Yukon |
geographic_facet |
Arctic Herschel Island Yukon |
genre |
Arctic Global warming Herschel Herschel Island permafrost Tundra Yukon |
genre_facet |
Arctic Global warming Herschel Herschel Island permafrost Tundra Yukon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885591 http://dx.doi.org/10.1371/journal.pone.0084761 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0084761 |
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PLoS ONE |
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9 |
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1 |
container_start_page |
e84761 |
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