Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus
Neurochemicals are likely to play key roles in physiological/behavioral control in the copepod crustacean Calanus finmarchicus, the biomass dominant zooplankton for much of the North Atlantic Ocean. Previously, a de novo assembled transcriptome consisting of 206,041 unique sequences was used to char...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872210 http://www.ncbi.nlm.nih.gov/pubmed/24148657 https://doi.org/10.1016/j.ygcen.2013.10.003 |
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ftpubmed:oai:pubmedcentral.nih.gov:3872210 2023-05-15T15:47:55+02:00 Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus Christie, Andrew E. Fontanilla, Tiana M. Roncalli, Vittoria Cieslak, Matthew C. Lenz, Petra H. 2013-10-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872210 http://www.ncbi.nlm.nih.gov/pubmed/24148657 https://doi.org/10.1016/j.ygcen.2013.10.003 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24148657 http://dx.doi.org/10.1016/j.ygcen.2013.10.003 © 2013 Elsevier Inc. All rights reserved. Article Text 2013 ftpubmed https://doi.org/10.1016/j.ygcen.2013.10.003 2015-01-04T00:56:34Z Neurochemicals are likely to play key roles in physiological/behavioral control in the copepod crustacean Calanus finmarchicus, the biomass dominant zooplankton for much of the North Atlantic Ocean. Previously, a de novo assembled transcriptome consisting of 206,041 unique sequences was used to characterize the peptidergic signaling systems of Calanus. Here, this assembly was mined for transcripts encoding enzymes involved in amine biosynthesis. Using known Drosophila melanogaster proteins as templates, transcripts encoding putative Calanus homologs of tryptophan-phenylalanine hydroxylase (dopamine, octopamine and serotonin biosynthesis), tyrosine hydroxylase (dopamine biosynthesis), DOPA decarboxylase (dopamine and serotonin biosynthesis), histidine decarboxylase (histamine biosynthesis), tyrosine decarboxylase (octopamine biosynthesis), tyramine β-hydroxylase (octopamine biosynthesis) and tryptophan hydroxylase (serotonin biosynthesis) were identified. Reverse BLAST and domain analyses show that the proteins deduced from these transcripts possess sequence homology to and the structural hallmarks of their respective enzyme families. Developmental profiling revealed a remarkably consistent pattern of expression for all transcripts, with the highest levels of expression typically seen in the early nauplius and early copepodite. These expression patterns suggest roles for amines during development, particularly in the metamorphic transitions from embryo to nauplius and from nauplius to copepodite. Taken collectively, the data presented here lay a strong foundation for future gene-based studies of aminergic signaling in this and other copepod species, in particular assessment of the roles they may play in developmental control. Text Calanus finmarchicus North Atlantic PubMed Central (PMC) General and Comparative Endocrinology 195 28 39 |
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Article Christie, Andrew E. Fontanilla, Tiana M. Roncalli, Vittoria Cieslak, Matthew C. Lenz, Petra H. Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus |
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description |
Neurochemicals are likely to play key roles in physiological/behavioral control in the copepod crustacean Calanus finmarchicus, the biomass dominant zooplankton for much of the North Atlantic Ocean. Previously, a de novo assembled transcriptome consisting of 206,041 unique sequences was used to characterize the peptidergic signaling systems of Calanus. Here, this assembly was mined for transcripts encoding enzymes involved in amine biosynthesis. Using known Drosophila melanogaster proteins as templates, transcripts encoding putative Calanus homologs of tryptophan-phenylalanine hydroxylase (dopamine, octopamine and serotonin biosynthesis), tyrosine hydroxylase (dopamine biosynthesis), DOPA decarboxylase (dopamine and serotonin biosynthesis), histidine decarboxylase (histamine biosynthesis), tyrosine decarboxylase (octopamine biosynthesis), tyramine β-hydroxylase (octopamine biosynthesis) and tryptophan hydroxylase (serotonin biosynthesis) were identified. Reverse BLAST and domain analyses show that the proteins deduced from these transcripts possess sequence homology to and the structural hallmarks of their respective enzyme families. Developmental profiling revealed a remarkably consistent pattern of expression for all transcripts, with the highest levels of expression typically seen in the early nauplius and early copepodite. These expression patterns suggest roles for amines during development, particularly in the metamorphic transitions from embryo to nauplius and from nauplius to copepodite. Taken collectively, the data presented here lay a strong foundation for future gene-based studies of aminergic signaling in this and other copepod species, in particular assessment of the roles they may play in developmental control. |
format |
Text |
author |
Christie, Andrew E. Fontanilla, Tiana M. Roncalli, Vittoria Cieslak, Matthew C. Lenz, Petra H. |
author_facet |
Christie, Andrew E. Fontanilla, Tiana M. Roncalli, Vittoria Cieslak, Matthew C. Lenz, Petra H. |
author_sort |
Christie, Andrew E. |
title |
Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus |
title_short |
Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus |
title_full |
Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus |
title_fullStr |
Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus |
title_full_unstemmed |
Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus |
title_sort |
identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean calanus finmarchicus |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872210 http://www.ncbi.nlm.nih.gov/pubmed/24148657 https://doi.org/10.1016/j.ygcen.2013.10.003 |
genre |
Calanus finmarchicus North Atlantic |
genre_facet |
Calanus finmarchicus North Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/24148657 http://dx.doi.org/10.1016/j.ygcen.2013.10.003 |
op_rights |
© 2013 Elsevier Inc. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.ygcen.2013.10.003 |
container_title |
General and Comparative Endocrinology |
container_volume |
195 |
container_start_page |
28 |
op_container_end_page |
39 |
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1766382900625801216 |