Bacterial genome replication at subzero temperatures in permafrost
Microbial metabolic activity occurs at subzero temperatures in permafrost, an environment representing ∼25% of the global soil organic matter. Although much of the observed subzero microbial activity may be due to basal metabolism or macromolecular repair, there is also ample evidence for cellular g...
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ftpubmed:oai:pubmedcentral.nih.gov:3869017 2023-05-15T17:56:36+02:00 Bacterial genome replication at subzero temperatures in permafrost Tuorto, Steven J Darias, Phillip McGuinness, Lora R Panikov, Nicolai Zhang, Tingjun Häggblom, Max M Kerkhof, Lee J 2014-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869017 http://www.ncbi.nlm.nih.gov/pubmed/23985750 https://doi.org/10.1038/ismej.2013.140 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23985750 http://dx.doi.org/10.1038/ismej.2013.140 Copyright © 2014 International Society for Microbial Ecology Original Article Text 2014 ftpubmed https://doi.org/10.1038/ismej.2013.140 2015-01-04T00:55:31Z Microbial metabolic activity occurs at subzero temperatures in permafrost, an environment representing ∼25% of the global soil organic matter. Although much of the observed subzero microbial activity may be due to basal metabolism or macromolecular repair, there is also ample evidence for cellular growth. Unfortunately, most metabolic measurements or culture-based laboratory experiments cannot elucidate the specific microorganisms responsible for metabolic activities in native permafrost, nor, can bulk approaches determine whether different members of the microbial community modulate their responses as a function of changing subzero temperatures. Here, we report on the use of stable isotope probing with 13C-acetate to demonstrate bacterial genome replication in Alaskan permafrost at temperatures of 0 to −20 °C. We found that the majority (80%) of operational taxonomic units detected in permafrost microcosms were active and could synthesize 13C-labeled DNA when supplemented with 13C-acetate at temperatures of 0 to −20 °C during a 6-month incubation. The data indicated that some members of the bacterial community were active across all of the experimental temperatures, whereas many others only synthesized DNA within a narrow subzero temperature range. Phylogenetic analysis of 13C-labeled 16S rRNA genes revealed that the subzero active bacteria were members of the Acidobacteria, Actinobacteria, Chloroflexi, Gemmatimonadetes and Proteobacteria phyla and were distantly related to currently cultivated psychrophiles. These results imply that small subzero temperature changes may lead to changes in the active microbial community, which could have consequences for biogeochemical cycling in permanently frozen systems. Text permafrost PubMed Central (PMC) The ISME Journal 8 1 139 149 |
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Original Article Tuorto, Steven J Darias, Phillip McGuinness, Lora R Panikov, Nicolai Zhang, Tingjun Häggblom, Max M Kerkhof, Lee J Bacterial genome replication at subzero temperatures in permafrost |
topic_facet |
Original Article |
description |
Microbial metabolic activity occurs at subzero temperatures in permafrost, an environment representing ∼25% of the global soil organic matter. Although much of the observed subzero microbial activity may be due to basal metabolism or macromolecular repair, there is also ample evidence for cellular growth. Unfortunately, most metabolic measurements or culture-based laboratory experiments cannot elucidate the specific microorganisms responsible for metabolic activities in native permafrost, nor, can bulk approaches determine whether different members of the microbial community modulate their responses as a function of changing subzero temperatures. Here, we report on the use of stable isotope probing with 13C-acetate to demonstrate bacterial genome replication in Alaskan permafrost at temperatures of 0 to −20 °C. We found that the majority (80%) of operational taxonomic units detected in permafrost microcosms were active and could synthesize 13C-labeled DNA when supplemented with 13C-acetate at temperatures of 0 to −20 °C during a 6-month incubation. The data indicated that some members of the bacterial community were active across all of the experimental temperatures, whereas many others only synthesized DNA within a narrow subzero temperature range. Phylogenetic analysis of 13C-labeled 16S rRNA genes revealed that the subzero active bacteria were members of the Acidobacteria, Actinobacteria, Chloroflexi, Gemmatimonadetes and Proteobacteria phyla and were distantly related to currently cultivated psychrophiles. These results imply that small subzero temperature changes may lead to changes in the active microbial community, which could have consequences for biogeochemical cycling in permanently frozen systems. |
format |
Text |
author |
Tuorto, Steven J Darias, Phillip McGuinness, Lora R Panikov, Nicolai Zhang, Tingjun Häggblom, Max M Kerkhof, Lee J |
author_facet |
Tuorto, Steven J Darias, Phillip McGuinness, Lora R Panikov, Nicolai Zhang, Tingjun Häggblom, Max M Kerkhof, Lee J |
author_sort |
Tuorto, Steven J |
title |
Bacterial genome replication at subzero temperatures in permafrost |
title_short |
Bacterial genome replication at subzero temperatures in permafrost |
title_full |
Bacterial genome replication at subzero temperatures in permafrost |
title_fullStr |
Bacterial genome replication at subzero temperatures in permafrost |
title_full_unstemmed |
Bacterial genome replication at subzero temperatures in permafrost |
title_sort |
bacterial genome replication at subzero temperatures in permafrost |
publisher |
Nature Publishing Group |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869017 http://www.ncbi.nlm.nih.gov/pubmed/23985750 https://doi.org/10.1038/ismej.2013.140 |
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permafrost |
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permafrost |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23985750 http://dx.doi.org/10.1038/ismej.2013.140 |
op_rights |
Copyright © 2014 International Society for Microbial Ecology |
op_doi |
https://doi.org/10.1038/ismej.2013.140 |
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The ISME Journal |
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8 |
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139 |
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149 |
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1766164806189973504 |