Characterization and allelic variation of the transporters associated with antigen processing (TAP) genes in the domestic dog (Canis lupus familiaris)
The function of the transporters associated with antigen processing (TAP) complex is to shuttle antigenic peptides from the cytosol to the endoplasmic reticulum to load MHC class I molecules for CD8+ T-cell immunosurveillance. Here we report the promoter and coding regions of the canine TAP1 and TAP...
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ftpubmed:oai:pubmedcentral.nih.gov:3846772 2023-05-15T15:50:17+02:00 Characterization and allelic variation of the transporters associated with antigen processing (TAP) genes in the domestic dog (Canis lupus familiaris) Gojanovich, Gregory S. Ross, Peter Holmer, Savannah R. Holmes, Jennifer C. Hess, Paul R. 2013-07-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846772 http://www.ncbi.nlm.nih.gov/pubmed/23892057 https://doi.org/10.1016/j.dci.2013.07.011 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23892057 http://dx.doi.org/10.1016/j.dci.2013.07.011 © 2013 Elsevier Ltd. All rights reserved. Article Text 2013 ftpubmed https://doi.org/10.1016/j.dci.2013.07.011 2014-12-07T00:59:10Z The function of the transporters associated with antigen processing (TAP) complex is to shuttle antigenic peptides from the cytosol to the endoplasmic reticulum to load MHC class I molecules for CD8+ T-cell immunosurveillance. Here we report the promoter and coding regions of the canine TAP1 and TAP2 genes, which encode the homologous subunits forming the TAP heterodimer. By sampling genetically divergent breeds, polymorphisms in both genes were identified, although there were few amino acid differences between alleles. Splice variants were also found. When aligned to TAP genes of other species, functional regions appeared conserved, and upon phylogenetic analysis, canine sequences segregated appropriately with their orthologs. Transfer of the canine TAP2 gene into a murine TAP2-defective cell line rescued surface MHC class I expression, confirming exporter function. This data should prove useful in investigating the association of specific TAP defects or alleles with immunity to intracellular pathogens and cancer in dogs. Text Canis lupus PubMed Central (PMC) Developmental & Comparative Immunology 41 4 578 586 |
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Article Gojanovich, Gregory S. Ross, Peter Holmer, Savannah R. Holmes, Jennifer C. Hess, Paul R. Characterization and allelic variation of the transporters associated with antigen processing (TAP) genes in the domestic dog (Canis lupus familiaris) |
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description |
The function of the transporters associated with antigen processing (TAP) complex is to shuttle antigenic peptides from the cytosol to the endoplasmic reticulum to load MHC class I molecules for CD8+ T-cell immunosurveillance. Here we report the promoter and coding regions of the canine TAP1 and TAP2 genes, which encode the homologous subunits forming the TAP heterodimer. By sampling genetically divergent breeds, polymorphisms in both genes were identified, although there were few amino acid differences between alleles. Splice variants were also found. When aligned to TAP genes of other species, functional regions appeared conserved, and upon phylogenetic analysis, canine sequences segregated appropriately with their orthologs. Transfer of the canine TAP2 gene into a murine TAP2-defective cell line rescued surface MHC class I expression, confirming exporter function. This data should prove useful in investigating the association of specific TAP defects or alleles with immunity to intracellular pathogens and cancer in dogs. |
format |
Text |
author |
Gojanovich, Gregory S. Ross, Peter Holmer, Savannah R. Holmes, Jennifer C. Hess, Paul R. |
author_facet |
Gojanovich, Gregory S. Ross, Peter Holmer, Savannah R. Holmes, Jennifer C. Hess, Paul R. |
author_sort |
Gojanovich, Gregory S. |
title |
Characterization and allelic variation of the transporters associated with antigen processing (TAP) genes in the domestic dog (Canis lupus familiaris) |
title_short |
Characterization and allelic variation of the transporters associated with antigen processing (TAP) genes in the domestic dog (Canis lupus familiaris) |
title_full |
Characterization and allelic variation of the transporters associated with antigen processing (TAP) genes in the domestic dog (Canis lupus familiaris) |
title_fullStr |
Characterization and allelic variation of the transporters associated with antigen processing (TAP) genes in the domestic dog (Canis lupus familiaris) |
title_full_unstemmed |
Characterization and allelic variation of the transporters associated with antigen processing (TAP) genes in the domestic dog (Canis lupus familiaris) |
title_sort |
characterization and allelic variation of the transporters associated with antigen processing (tap) genes in the domestic dog (canis lupus familiaris) |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846772 http://www.ncbi.nlm.nih.gov/pubmed/23892057 https://doi.org/10.1016/j.dci.2013.07.011 |
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Canis lupus |
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Canis lupus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23892057 http://dx.doi.org/10.1016/j.dci.2013.07.011 |
op_rights |
© 2013 Elsevier Ltd. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.dci.2013.07.011 |
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Developmental & Comparative Immunology |
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41 |
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4 |
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578 |
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586 |
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1766385257957818368 |