Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme
Psychrophilic bacteria, which grow on lactose as a carbon source, were isolated from Antarctic polar sea water. Among the psychrophilic bacteria isolated, strain KNOUC808 was able to grow on lactose at below 5°C, and showed 0.867 unit of o-nitrophenyl β-D-galactopyranoside(ONPG) hydrolyzing activity...
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ftpubmed:oai:pubmedcentral.nih.gov:3768794 2023-05-15T13:34:08+02:00 Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme Nam, EunSook Ahn, JongKun 2011 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768794 http://www.ncbi.nlm.nih.gov/pubmed/24031708 https://doi.org/10.1590/S1517-838220110003000011 en eng Sociedade Brasileira de Microbiologia http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768794 http://www.ncbi.nlm.nih.gov/pubmed/24031708 http://dx.doi.org/10.1590/S1517-838220110003000011 © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY-NC Environmental Microbiology Text 2011 ftpubmed https://doi.org/10.1590/S1517-838220110003000011 2013-09-15T01:02:34Z Psychrophilic bacteria, which grow on lactose as a carbon source, were isolated from Antarctic polar sea water. Among the psychrophilic bacteria isolated, strain KNOUC808 was able to grow on lactose at below 5°C, and showed 0.867 unit of o-nitrophenyl β-D-galactopyranoside(ONPG) hydrolyzing activity at 4°C. The isolate was gram-negative, rod, aerobic, catalase positive and oxidase positive. Optimum growth was done at 20°C, pH 6.8–7.2. The composition of major fatty acids in cell of KNOUC801 was C12:0 (5.48%), C12:0 3OH (9.21%), C16:0 (41.83%), C17:0 ω8 (7.24%) and C18:1 ω7 (7.04%). All these results together suggest that it is affiliated with Pseudoalteromonas genus. The 16S rDNA sequence corroborate the phenotypic tests and the novel strain was designated as Pseudoalteromonas sp. KNOUC808. The optimum temperature and pH for lactose hydrolyzing enzyme was 20°C and 7.8, respectively. The enzyme was stable at 4°C for 7 days, but its activity decreased to about 50% of initial activity at 37°C in 7 days. Text Antarc* Antarctic PubMed Central (PMC) Antarctic |
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English |
topic |
Environmental Microbiology |
spellingShingle |
Environmental Microbiology Nam, EunSook Ahn, JongKun Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme |
topic_facet |
Environmental Microbiology |
description |
Psychrophilic bacteria, which grow on lactose as a carbon source, were isolated from Antarctic polar sea water. Among the psychrophilic bacteria isolated, strain KNOUC808 was able to grow on lactose at below 5°C, and showed 0.867 unit of o-nitrophenyl β-D-galactopyranoside(ONPG) hydrolyzing activity at 4°C. The isolate was gram-negative, rod, aerobic, catalase positive and oxidase positive. Optimum growth was done at 20°C, pH 6.8–7.2. The composition of major fatty acids in cell of KNOUC801 was C12:0 (5.48%), C12:0 3OH (9.21%), C16:0 (41.83%), C17:0 ω8 (7.24%) and C18:1 ω7 (7.04%). All these results together suggest that it is affiliated with Pseudoalteromonas genus. The 16S rDNA sequence corroborate the phenotypic tests and the novel strain was designated as Pseudoalteromonas sp. KNOUC808. The optimum temperature and pH for lactose hydrolyzing enzyme was 20°C and 7.8, respectively. The enzyme was stable at 4°C for 7 days, but its activity decreased to about 50% of initial activity at 37°C in 7 days. |
format |
Text |
author |
Nam, EunSook Ahn, JongKun |
author_facet |
Nam, EunSook Ahn, JongKun |
author_sort |
Nam, EunSook |
title |
Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme |
title_short |
Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme |
title_full |
Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme |
title_fullStr |
Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme |
title_full_unstemmed |
Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme |
title_sort |
antarctic marine bacterium pseudoalteromonas sp. knouc808 as a source of cold-adapted lactose hydrolyzing enzyme |
publisher |
Sociedade Brasileira de Microbiologia |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768794 http://www.ncbi.nlm.nih.gov/pubmed/24031708 https://doi.org/10.1590/S1517-838220110003000011 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768794 http://www.ncbi.nlm.nih.gov/pubmed/24031708 http://dx.doi.org/10.1590/S1517-838220110003000011 |
op_rights |
© Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1590/S1517-838220110003000011 |
_version_ |
1766049273958367232 |