Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica
The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-activ...
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Sociedade Brasileira de Microbiologia
2011
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ftpubmed:oai:pubmedcentral.nih.gov:3768780 2023-05-15T13:34:08+02:00 Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica Cotârleţ, Mihaela Negoiţă, Teodor Gh. Bahrim, Gabriela E. Stougaard, Peter 2011 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768780 http://www.ncbi.nlm.nih.gov/pubmed/24031702 https://doi.org/10.1590/S1517-83822011000300005 en eng Sociedade Brasileira de Microbiologia http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768780 http://www.ncbi.nlm.nih.gov/pubmed/24031702 http://dx.doi.org/10.1590/S1517-83822011000300005 © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY-NC Environmental Microbiology Text 2011 ftpubmed https://doi.org/10.1590/S1517-83822011000300005 2013-09-15T01:02:19Z The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20°C, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20°C. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures. Text Antarc* Antarctica East Antarctica PubMed Central (PMC) East Antarctica Norway Brazilian Journal of Microbiology 42 3 868 877 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Environmental Microbiology |
spellingShingle |
Environmental Microbiology Cotârleţ, Mihaela Negoiţă, Teodor Gh. Bahrim, Gabriela E. Stougaard, Peter Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
topic_facet |
Environmental Microbiology |
description |
The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20°C, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20°C. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures. |
format |
Text |
author |
Cotârleţ, Mihaela Negoiţă, Teodor Gh. Bahrim, Gabriela E. Stougaard, Peter |
author_facet |
Cotârleţ, Mihaela Negoiţă, Teodor Gh. Bahrim, Gabriela E. Stougaard, Peter |
author_sort |
Cotârleţ, Mihaela |
title |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_short |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_full |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_fullStr |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_full_unstemmed |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_sort |
partial characterization of cold active amylases and proteases of streptomyces sp. from antarctica |
publisher |
Sociedade Brasileira de Microbiologia |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768780 http://www.ncbi.nlm.nih.gov/pubmed/24031702 https://doi.org/10.1590/S1517-83822011000300005 |
geographic |
East Antarctica Norway |
geographic_facet |
East Antarctica Norway |
genre |
Antarc* Antarctica East Antarctica |
genre_facet |
Antarc* Antarctica East Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768780 http://www.ncbi.nlm.nih.gov/pubmed/24031702 http://dx.doi.org/10.1590/S1517-83822011000300005 |
op_rights |
© Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1590/S1517-83822011000300005 |
container_title |
Brazilian Journal of Microbiology |
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42 |
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3 |
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868 |
op_container_end_page |
877 |
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1766049271990190080 |