Expression at 279 K, purification, crystallization and preliminary X-ray crystallographic analysis of a novel cold-active β-1,4-d-mannanase from the Antarctic springtail Cryptopygus antarcticus

• Published in: Acta Crystallographica Section F Structural Biology and Crystallization Communications
• Main Authors: Kim, Min-Kyu, An, Young Jun, Jeong, Chang-Sook, Song, Jung Min, Kang, Mee Hye, Lee, Youn-Ho, Cha, Sun-Shin
• Format: Text
• Language: English
• Published: International Union of Crystallography 2013
• Subjects: Crystallization Communications; Antarctic; The Antarctic; Antarc*; Antarctic Springtail; antarcticus; Cryptopygus antarcticus; Springtail
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758150/; http://www.ncbi.nlm.nih.gov/pubmed/23989150; https://doi.org/10.1107/S1744309113020538

A cold-active β-1,4-d-mannanase from the Antarctic springtail C. antarcticus (CaMan) has been expressed, purified and crystallized. The CaMan crystal belonged to space group P212121, with unit-cell parameters a = 73.40, b = 83.81, c = 163.55 Å, and diffracted to 2.35 Å resolution. A 2.40 Å resolution data set was also collected from a CaMan–mannopentaose complex crystal.