Expression at 279 K, purification, crystallization and preliminary X-ray crystallographic analysis of a novel cold-active β-1,4-d-mannanase from the Antarctic springtail Cryptopygus antarcticus
A cold-active β-1,4-d-mannanase from the Antarctic springtail C. antarcticus (CaMan) has been expressed, purified and crystallized. The CaMan crystal belonged to space group P212121, with unit-cell parameters a = 73.40, b = 83.81, c = 163.55 Å, and diffracted to 2.35 Å resolution. A 2.40 Å resolutio...
Published in: | Acta Crystallographica Section F Structural Biology and Crystallization Communications |
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Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
International Union of Crystallography
2013
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Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758150/ http://www.ncbi.nlm.nih.gov/pubmed/23989150 https://doi.org/10.1107/S1744309113020538 |
Summary: | A cold-active β-1,4-d-mannanase from the Antarctic springtail C. antarcticus (CaMan) has been expressed, purified and crystallized. The CaMan crystal belonged to space group P212121, with unit-cell parameters a = 73.40, b = 83.81, c = 163.55 Å, and diffracted to 2.35 Å resolution. A 2.40 Å resolution data set was also collected from a CaMan–mannopentaose complex crystal. |
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