pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment
pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replicatio...
Published in: | PLoS ONE |
---|---|
Main Authors: | , |
Format: | Text |
Language: | English |
Published: |
Public Library of Science
2013
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756968 http://www.ncbi.nlm.nih.gov/pubmed/24009682 https://doi.org/10.1371/journal.pone.0072461 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:3756968 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:3756968 2023-05-15T13:49:12+02:00 pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment Guo, Suxia Mahillon, Jacques 2013-08-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756968 http://www.ncbi.nlm.nih.gov/pubmed/24009682 https://doi.org/10.1371/journal.pone.0072461 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756968 http://www.ncbi.nlm.nih.gov/pubmed/24009682 http://dx.doi.org/10.1371/journal.pone.0072461 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2013 ftpubmed https://doi.org/10.1371/journal.pone.0072461 2013-09-15T00:30:35Z pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replication region, this plasmid contains the genetic determinants for cadmium and arsenic resistances, putative methyltransferase, tyrosine recombinase, spore coat protein and potassium transport protein, as well as several hypothetical proteins. Cloning the pGIAK1 cad operon in Bacillus cereus H3081.97 and its ars operon in Bacillus subtilis 1A280 conferred to these hosts cadmium and arsenic resistances, respectively, therefore confirming their bona fide activities. The pGIAK1 replicon region was also shown to be functional in Bacillus thuringiensis, Bacillus subtilis and Staphylococcus aureus, but was only stably maintained in B. subtilis. Finally, using an Escherichia coli - B. thuringiensis shuttle BAC vector, pGIAK1 was shown to display conjugative properties since it was able to transfer the BAC plasmid among B. thuringiensis strains. Text Antarc* Antarctic PubMed Central (PMC) Antarctic Concordia Station ENVELOPE(123.333,123.333,-75.100,-75.100) The Antarctic PLoS ONE 8 8 e72461 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Guo, Suxia Mahillon, Jacques pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
topic_facet |
Research Article |
description |
pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replication region, this plasmid contains the genetic determinants for cadmium and arsenic resistances, putative methyltransferase, tyrosine recombinase, spore coat protein and potassium transport protein, as well as several hypothetical proteins. Cloning the pGIAK1 cad operon in Bacillus cereus H3081.97 and its ars operon in Bacillus subtilis 1A280 conferred to these hosts cadmium and arsenic resistances, respectively, therefore confirming their bona fide activities. The pGIAK1 replicon region was also shown to be functional in Bacillus thuringiensis, Bacillus subtilis and Staphylococcus aureus, but was only stably maintained in B. subtilis. Finally, using an Escherichia coli - B. thuringiensis shuttle BAC vector, pGIAK1 was shown to display conjugative properties since it was able to transfer the BAC plasmid among B. thuringiensis strains. |
format |
Text |
author |
Guo, Suxia Mahillon, Jacques |
author_facet |
Guo, Suxia Mahillon, Jacques |
author_sort |
Guo, Suxia |
title |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_short |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_full |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_fullStr |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_full_unstemmed |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_sort |
pgiak1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the antarctic concordia station confined environment |
publisher |
Public Library of Science |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756968 http://www.ncbi.nlm.nih.gov/pubmed/24009682 https://doi.org/10.1371/journal.pone.0072461 |
long_lat |
ENVELOPE(123.333,123.333,-75.100,-75.100) |
geographic |
Antarctic Concordia Station The Antarctic |
geographic_facet |
Antarctic Concordia Station The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756968 http://www.ncbi.nlm.nih.gov/pubmed/24009682 http://dx.doi.org/10.1371/journal.pone.0072461 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0072461 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
8 |
container_start_page |
e72461 |
_version_ |
1766250988574867456 |