Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa
A genomic island consisting of 14 open reading frames, orfA to orfN was previously identified in Pseudomonas aeruginosa strain PAK and shown to be essential for glycosylation of flagellin. DNA microarray hybridization analysis of a number of P. aeruginosa strains from diverse origins showed that thi...
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ftpubmed:oai:pubmedcentral.nih.gov:374406 2023-05-15T18:19:22+02:00 Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa Arora, Shiwani K. Wolfgang, Matthew C. Lory, Stephen Ramphal, Reuben 2004-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC374406 http://www.ncbi.nlm.nih.gov/pubmed/15028697 https://doi.org/10.1128/JB.186.7.2115-2122.2004 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC374406 http://www.ncbi.nlm.nih.gov/pubmed/15028697 http://dx.doi.org/10.1128/JB.186.7.2115-2122.2004 Copyright © 2004, American Society for Microbiology Genetics and Molecular Biology Text 2004 ftpubmed https://doi.org/10.1128/JB.186.7.2115-2122.2004 2013-08-29T22:01:05Z A genomic island consisting of 14 open reading frames, orfA to orfN was previously identified in Pseudomonas aeruginosa strain PAK and shown to be essential for glycosylation of flagellin. DNA microarray hybridization analysis of a number of P. aeruginosa strains from diverse origins showed that this island is polymorphic. PCR and sequence analysis confirmed that many P. aeruginosa strains carry an abbreviated version of the island (short island) in which orfD, -E and -H are polymorphic and orfI, -J, -K, -L, and -M are absent. To ascertain whether there was a relationship between the inheritance of the short island and specific flagellin sequence variants, complete or partial nucleotide sequences of flagellin genes from 24 a-type P. aeruginosa strains were determined. Two distinct flagellin subtypes, designated A1 and A2, were apparent. Strains with the complete 14-gene island (long island) were almost exclusively of the A1 type, whereas strains carrying the short island were associated with both A1- and A2-type flagellins. These findings indicate that P. aeruginosa possesses a relatively low number of distinct flagellin types and probably has the capacity to further diversify this antigenic surface protein by glycosylation. Text Short Island PubMed Central (PMC) Long Island Short Island ENVELOPE(-60.400,-60.400,-63.950,-63.950) Journal of Bacteriology 186 7 2115 2122 |
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Genetics and Molecular Biology |
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Genetics and Molecular Biology Arora, Shiwani K. Wolfgang, Matthew C. Lory, Stephen Ramphal, Reuben Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
topic_facet |
Genetics and Molecular Biology |
description |
A genomic island consisting of 14 open reading frames, orfA to orfN was previously identified in Pseudomonas aeruginosa strain PAK and shown to be essential for glycosylation of flagellin. DNA microarray hybridization analysis of a number of P. aeruginosa strains from diverse origins showed that this island is polymorphic. PCR and sequence analysis confirmed that many P. aeruginosa strains carry an abbreviated version of the island (short island) in which orfD, -E and -H are polymorphic and orfI, -J, -K, -L, and -M are absent. To ascertain whether there was a relationship between the inheritance of the short island and specific flagellin sequence variants, complete or partial nucleotide sequences of flagellin genes from 24 a-type P. aeruginosa strains were determined. Two distinct flagellin subtypes, designated A1 and A2, were apparent. Strains with the complete 14-gene island (long island) were almost exclusively of the A1 type, whereas strains carrying the short island were associated with both A1- and A2-type flagellins. These findings indicate that P. aeruginosa possesses a relatively low number of distinct flagellin types and probably has the capacity to further diversify this antigenic surface protein by glycosylation. |
format |
Text |
author |
Arora, Shiwani K. Wolfgang, Matthew C. Lory, Stephen Ramphal, Reuben |
author_facet |
Arora, Shiwani K. Wolfgang, Matthew C. Lory, Stephen Ramphal, Reuben |
author_sort |
Arora, Shiwani K. |
title |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_short |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_full |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_fullStr |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_full_unstemmed |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_sort |
sequence polymorphism in the glycosylation island and flagellins of pseudomonas aeruginosa |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC374406 http://www.ncbi.nlm.nih.gov/pubmed/15028697 https://doi.org/10.1128/JB.186.7.2115-2122.2004 |
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ENVELOPE(-60.400,-60.400,-63.950,-63.950) |
geographic |
Long Island Short Island |
geographic_facet |
Long Island Short Island |
genre |
Short Island |
genre_facet |
Short Island |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC374406 http://www.ncbi.nlm.nih.gov/pubmed/15028697 http://dx.doi.org/10.1128/JB.186.7.2115-2122.2004 |
op_rights |
Copyright © 2004, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/JB.186.7.2115-2122.2004 |
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Journal of Bacteriology |
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186 |
container_issue |
7 |
container_start_page |
2115 |
op_container_end_page |
2122 |
_version_ |
1766196443165491200 |