Subglacial Lake Vostok (Antarctica) Accretion Ice Contains a Diverse Set of Sequences from Aquatic, Marine and Sediment-Inhabiting Bacteria and Eukarya
Lake Vostok, the 7th largest (by volume) and 4th deepest lake on Earth, is covered by more than 3,700 m of ice, making it the largest subglacial lake known. The combination of cold, heat (from possible hydrothermal activity), pressure (from the overriding glacier), limited nutrients and complete dar...
Published in: | PLoS ONE |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
Public Library of Science
2013
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700977 http://www.ncbi.nlm.nih.gov/pubmed/23843994 https://doi.org/10.1371/journal.pone.0067221 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:3700977 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:3700977 2023-05-15T14:02:23+02:00 Subglacial Lake Vostok (Antarctica) Accretion Ice Contains a Diverse Set of Sequences from Aquatic, Marine and Sediment-Inhabiting Bacteria and Eukarya Shtarkman, Yury M. Koçer, Zeynep A. Edgar, Robyn Veerapaneni, Ram S. D’Elia, Tom Morris, Paul F. Rogers, Scott O. 2013-07-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700977 http://www.ncbi.nlm.nih.gov/pubmed/23843994 https://doi.org/10.1371/journal.pone.0067221 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700977 http://www.ncbi.nlm.nih.gov/pubmed/23843994 http://dx.doi.org/10.1371/journal.pone.0067221 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2013 ftpubmed https://doi.org/10.1371/journal.pone.0067221 2013-09-05T01:59:19Z Lake Vostok, the 7th largest (by volume) and 4th deepest lake on Earth, is covered by more than 3,700 m of ice, making it the largest subglacial lake known. The combination of cold, heat (from possible hydrothermal activity), pressure (from the overriding glacier), limited nutrients and complete darkness presents extreme challenges to life. Here, we report metagenomic/metatranscriptomic sequence analyses from four accretion ice sections from the Vostok 5G ice core. Two sections accreted in the vicinity of an embayment on the southwestern end of the lake, and the other two represented part of the southern main basin. We obtained 3,507 unique gene sequences from concentrates of 500 ml of 0.22 µm-filtered accretion ice meltwater. Taxonomic classifications (to genus and/or species) were possible for 1,623 of the sequences. Species determinations in combination with mRNA gene sequence results allowed deduction of the metabolic pathways represented in the accretion ice and, by extension, in the lake. Approximately 94% of the sequences were from Bacteria and 6% were from Eukarya. Only two sequences were from Archaea. In general, the taxa were similar to organisms previously described from lakes, brackish water, marine environments, soil, glaciers, ice, lake sediments, deep-sea sediments, deep-sea thermal vents, animals and plants. Sequences from aerobic, anaerobic, psychrophilic, thermophilic, halophilic, alkaliphilic, acidophilic, desiccation-resistant, autotrophic and heterotrophic organisms were present, including a number from multicellular eukaryotes. Text Antarc* Antarctica ice core PubMed Central (PMC) Lake Vostok ENVELOPE(106.000,106.000,-77.500,-77.500) PLoS ONE 8 7 e67221 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Shtarkman, Yury M. Koçer, Zeynep A. Edgar, Robyn Veerapaneni, Ram S. D’Elia, Tom Morris, Paul F. Rogers, Scott O. Subglacial Lake Vostok (Antarctica) Accretion Ice Contains a Diverse Set of Sequences from Aquatic, Marine and Sediment-Inhabiting Bacteria and Eukarya |
topic_facet |
Research Article |
description |
Lake Vostok, the 7th largest (by volume) and 4th deepest lake on Earth, is covered by more than 3,700 m of ice, making it the largest subglacial lake known. The combination of cold, heat (from possible hydrothermal activity), pressure (from the overriding glacier), limited nutrients and complete darkness presents extreme challenges to life. Here, we report metagenomic/metatranscriptomic sequence analyses from four accretion ice sections from the Vostok 5G ice core. Two sections accreted in the vicinity of an embayment on the southwestern end of the lake, and the other two represented part of the southern main basin. We obtained 3,507 unique gene sequences from concentrates of 500 ml of 0.22 µm-filtered accretion ice meltwater. Taxonomic classifications (to genus and/or species) were possible for 1,623 of the sequences. Species determinations in combination with mRNA gene sequence results allowed deduction of the metabolic pathways represented in the accretion ice and, by extension, in the lake. Approximately 94% of the sequences were from Bacteria and 6% were from Eukarya. Only two sequences were from Archaea. In general, the taxa were similar to organisms previously described from lakes, brackish water, marine environments, soil, glaciers, ice, lake sediments, deep-sea sediments, deep-sea thermal vents, animals and plants. Sequences from aerobic, anaerobic, psychrophilic, thermophilic, halophilic, alkaliphilic, acidophilic, desiccation-resistant, autotrophic and heterotrophic organisms were present, including a number from multicellular eukaryotes. |
format |
Text |
author |
Shtarkman, Yury M. Koçer, Zeynep A. Edgar, Robyn Veerapaneni, Ram S. D’Elia, Tom Morris, Paul F. Rogers, Scott O. |
author_facet |
Shtarkman, Yury M. Koçer, Zeynep A. Edgar, Robyn Veerapaneni, Ram S. D’Elia, Tom Morris, Paul F. Rogers, Scott O. |
author_sort |
Shtarkman, Yury M. |
title |
Subglacial Lake Vostok (Antarctica) Accretion Ice Contains a Diverse Set of Sequences from Aquatic, Marine and Sediment-Inhabiting Bacteria and Eukarya |
title_short |
Subglacial Lake Vostok (Antarctica) Accretion Ice Contains a Diverse Set of Sequences from Aquatic, Marine and Sediment-Inhabiting Bacteria and Eukarya |
title_full |
Subglacial Lake Vostok (Antarctica) Accretion Ice Contains a Diverse Set of Sequences from Aquatic, Marine and Sediment-Inhabiting Bacteria and Eukarya |
title_fullStr |
Subglacial Lake Vostok (Antarctica) Accretion Ice Contains a Diverse Set of Sequences from Aquatic, Marine and Sediment-Inhabiting Bacteria and Eukarya |
title_full_unstemmed |
Subglacial Lake Vostok (Antarctica) Accretion Ice Contains a Diverse Set of Sequences from Aquatic, Marine and Sediment-Inhabiting Bacteria and Eukarya |
title_sort |
subglacial lake vostok (antarctica) accretion ice contains a diverse set of sequences from aquatic, marine and sediment-inhabiting bacteria and eukarya |
publisher |
Public Library of Science |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700977 http://www.ncbi.nlm.nih.gov/pubmed/23843994 https://doi.org/10.1371/journal.pone.0067221 |
long_lat |
ENVELOPE(106.000,106.000,-77.500,-77.500) |
geographic |
Lake Vostok |
geographic_facet |
Lake Vostok |
genre |
Antarc* Antarctica ice core |
genre_facet |
Antarc* Antarctica ice core |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700977 http://www.ncbi.nlm.nih.gov/pubmed/23843994 http://dx.doi.org/10.1371/journal.pone.0067221 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0067221 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
7 |
container_start_page |
e67221 |
_version_ |
1766272629254127616 |